High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams
Kato, Miyazaki, Hamaguchi et al. report the structure of Photosystem II in solution at 1.95 Å resolution by single-particle cryo-electron microscopy. They find that reducing the electron beam dosage decreases the electron beam damage while keeping the resolution of the cryo-EM structure, providing i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-03-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-01919-3 |
| _version_ | 1818430780083273728 |
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| author | Koji Kato Naoyuki Miyazaki Tasuku Hamaguchi Yoshiki Nakajima Fusamichi Akita Koji Yonekura Jian-Ren Shen |
| author_facet | Koji Kato Naoyuki Miyazaki Tasuku Hamaguchi Yoshiki Nakajima Fusamichi Akita Koji Yonekura Jian-Ren Shen |
| author_sort | Koji Kato |
| collection | DOAJ |
| description | Kato, Miyazaki, Hamaguchi et al. report the structure of Photosystem II in solution at 1.95 Å resolution by single-particle cryo-electron microscopy. They find that reducing the electron beam dosage decreases the electron beam damage while keeping the resolution of the cryo-EM structure, providing insights into the best practice for the determination of cryo-EM structures. |
| first_indexed | 2024-12-14T15:38:51Z |
| format | Article |
| id | doaj.art-3d455b2a1f944fa7b8cadda140da7248 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-14T15:38:51Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-3d455b2a1f944fa7b8cadda140da72482022-12-21T22:55:40ZengNature PortfolioCommunications Biology2399-36422021-03-014111110.1038/s42003-021-01919-3High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beamsKoji Kato0Naoyuki Miyazaki1Tasuku Hamaguchi2Yoshiki Nakajima3Fusamichi Akita4Koji Yonekura5Jian-Ren Shen6Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama UniversityLife Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of TsukubaBiostructural Mechanism Laboratory, RIKEN Spring-8 CenterResearch Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama UniversityResearch Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama UniversityBiostructural Mechanism Laboratory, RIKEN Spring-8 CenterResearch Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama UniversityKato, Miyazaki, Hamaguchi et al. report the structure of Photosystem II in solution at 1.95 Å resolution by single-particle cryo-electron microscopy. They find that reducing the electron beam dosage decreases the electron beam damage while keeping the resolution of the cryo-EM structure, providing insights into the best practice for the determination of cryo-EM structures.https://doi.org/10.1038/s42003-021-01919-3 |
| spellingShingle | Koji Kato Naoyuki Miyazaki Tasuku Hamaguchi Yoshiki Nakajima Fusamichi Akita Koji Yonekura Jian-Ren Shen High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams Communications Biology |
| title | High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams |
| title_full | High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams |
| title_fullStr | High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams |
| title_full_unstemmed | High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams |
| title_short | High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams |
| title_sort | high resolution cryo em structure of photosystem ii reveals damage from high dose electron beams |
| url | https://doi.org/10.1038/s42003-021-01919-3 |
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