Impact of distillers grain solids (DGS) and seasonality on the prevalence of Escherichia coli O157 at an abattoir in the U. S. Upper Midwest
Enterohaemorrhagic Escherichia coli (EHEC) serotype O157:H7 is carried asymptomatically by cattle gastrointestinal tract and the inclusion of distillers grains solids (DGS) in feed is thought to be a factor in the prevalence and persistence of EHEC O157 in a herd. The present study surveys the faeca...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09712119.2017.1288628 |
| _version_ | 1818312918523969536 |
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| author | Ryan C. Fink Jackie M. Popowski Jon E. Anderson Johanna L. Tran Sudha Kalyanikutty Grant I. Crawford Alfredo DiCostanzo Ryan B. Cox Francisco Diez-Gonzalez |
| author_facet | Ryan C. Fink Jackie M. Popowski Jon E. Anderson Johanna L. Tran Sudha Kalyanikutty Grant I. Crawford Alfredo DiCostanzo Ryan B. Cox Francisco Diez-Gonzalez |
| author_sort | Ryan C. Fink |
| collection | DOAJ |
| description | Enterohaemorrhagic Escherichia coli (EHEC) serotype O157:H7 is carried asymptomatically by cattle gastrointestinal tract and the inclusion of distillers grains solids (DGS) in feed is thought to be a factor in the prevalence and persistence of EHEC O157 in a herd. The present study surveys the faecal prevalence of E. coli O157 in cattle processed at an abattoir in the Upper Midwest and its association with environmental factors and feeding practices. Faecal samples were collected from pre-processing cows during a 1-year period. E. coli O157 prevalence was estimated isolation of putative positives and confirmation of isolates by immunoassay and multiplex virulence genes PCR analysis. Overall, E. coli O157 was confirmed in 11.2% of samples. Prevalence during winter was the highest at 14% followed by summer (11.6%) and declined to less than 8% the rest of the year. Winter was the only season that had a statistically significant effect on prevalence. As a category unto itself, DGS feeding before arrival had no significant influence on faecal prevalence. However, we found a significant interaction of DGS feeding and summer. This observation is extremely relevant because it corroborates a previous study and suggests possible feeding practices to abate EHEC O157 presence during harvest. |
| first_indexed | 2024-12-13T08:25:25Z |
| format | Article |
| id | doaj.art-3d47b8110f894c82abcbec6c472cdd40 |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-12-13T08:25:25Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-3d47b8110f894c82abcbec6c472cdd402022-12-21T23:53:54ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442018-01-0146123724110.1080/09712119.2017.12886281288628Impact of distillers grain solids (DGS) and seasonality on the prevalence of Escherichia coli O157 at an abattoir in the U. S. Upper MidwestRyan C. Fink0Jackie M. Popowski1Jon E. Anderson2Johanna L. Tran3Sudha Kalyanikutty4Grant I. Crawford5Alfredo DiCostanzo6Ryan B. Cox7Francisco Diez-Gonzalez8University of MinnesotaUniversity of MinnesotaUniversity of MinnesotaUniversity of MinnesotaUniversity of MinnesotaUniversity of MinnesotaUniversity of MinnesotaUniversity of MinnesotaUniversity of MinnesotaEnterohaemorrhagic Escherichia coli (EHEC) serotype O157:H7 is carried asymptomatically by cattle gastrointestinal tract and the inclusion of distillers grains solids (DGS) in feed is thought to be a factor in the prevalence and persistence of EHEC O157 in a herd. The present study surveys the faecal prevalence of E. coli O157 in cattle processed at an abattoir in the Upper Midwest and its association with environmental factors and feeding practices. Faecal samples were collected from pre-processing cows during a 1-year period. E. coli O157 prevalence was estimated isolation of putative positives and confirmation of isolates by immunoassay and multiplex virulence genes PCR analysis. Overall, E. coli O157 was confirmed in 11.2% of samples. Prevalence during winter was the highest at 14% followed by summer (11.6%) and declined to less than 8% the rest of the year. Winter was the only season that had a statistically significant effect on prevalence. As a category unto itself, DGS feeding before arrival had no significant influence on faecal prevalence. However, we found a significant interaction of DGS feeding and summer. This observation is extremely relevant because it corroborates a previous study and suggests possible feeding practices to abate EHEC O157 presence during harvest.http://dx.doi.org/10.1080/09712119.2017.1288628Foodborne pathogenscattleSTECO157food safetydistiller’s grains soluble |
| spellingShingle | Ryan C. Fink Jackie M. Popowski Jon E. Anderson Johanna L. Tran Sudha Kalyanikutty Grant I. Crawford Alfredo DiCostanzo Ryan B. Cox Francisco Diez-Gonzalez Impact of distillers grain solids (DGS) and seasonality on the prevalence of Escherichia coli O157 at an abattoir in the U. S. Upper Midwest Journal of Applied Animal Research Foodborne pathogens cattle STEC O157 food safety distiller’s grains soluble |
| title | Impact of distillers grain solids (DGS) and seasonality on the prevalence of Escherichia coli O157 at an abattoir in the U. S. Upper Midwest |
| title_full | Impact of distillers grain solids (DGS) and seasonality on the prevalence of Escherichia coli O157 at an abattoir in the U. S. Upper Midwest |
| title_fullStr | Impact of distillers grain solids (DGS) and seasonality on the prevalence of Escherichia coli O157 at an abattoir in the U. S. Upper Midwest |
| title_full_unstemmed | Impact of distillers grain solids (DGS) and seasonality on the prevalence of Escherichia coli O157 at an abattoir in the U. S. Upper Midwest |
| title_short | Impact of distillers grain solids (DGS) and seasonality on the prevalence of Escherichia coli O157 at an abattoir in the U. S. Upper Midwest |
| title_sort | impact of distillers grain solids dgs and seasonality on the prevalence of escherichia coli o157 at an abattoir in the u s upper midwest |
| topic | Foodborne pathogens cattle STEC O157 food safety distiller’s grains soluble |
| url | http://dx.doi.org/10.1080/09712119.2017.1288628 |
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