Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging
Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Tr...
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| Format: | Article |
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MDPI AG
2016-07-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/6/7/130 |
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| author | Yinxue Zu Jingran Bi Huiping Yan Haitao Wang Yukun Song Bei-Wei Zhu Mingqian Tan |
| author_facet | Yinxue Zu Jingran Bi Huiping Yan Haitao Wang Yukun Song Bei-Wei Zhu Mingqian Tan |
| author_sort | Yinxue Zu |
| collection | DOAJ |
| description | Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Transmission electron microscopy (TEM) demonstrated that the average particle sizes are 14 nm and 75 nm for starch and chitosan NSs, respectively. Fourier transform infrared (FT-IR) spectroscopy analysis showed that there are a large number of hydroxyl or amino groups on the surface of these polysaccharide-based NSs. Strong fluorescence with an excitation-dependent emission behaviour was observed under ultraviolet excitation. Interestingly, the photostability of the NSs was found to be superior to fluorescein and rhodamine B. The quantum yield of starch NSs could reach 11.12% under the excitation of 360 nm. The oxidative metal ions including Cu(II), Hg(II)and Fe(III) exhibited a quench effect on the fluorescence intensity of the prepared NSs. Both of the two kinds of the multicoloured NSs showed a maximum fluorescence intensity at pH 7, while the fluorescence intensity decreased dramatically when they were put in an either acidic or basic environment (at pH 3 or 11). The cytotoxicity study of starch NSs showed that low cell cytotoxicity and 80% viability was found after 24 h incubation, when their concentration was less than 10 mg/mL. The study also showed the possibility of using the multicoloured starch NSs for mouse melanoma cells and guppy fish imaging. |
| first_indexed | 2024-12-11T21:18:50Z |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-11T21:18:50Z |
| publishDate | 2016-07-01 |
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| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-3d494959f02a4f13834d4b989e1666cf2022-12-22T00:50:30ZengMDPI AGNanomaterials2079-49912016-07-016713010.3390/nano6070130nano6070130Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-ImagingYinxue Zu0Jingran Bi1Huiping Yan2Haitao Wang3Yukun Song4Bei-Wei Zhu5Mingqian Tan6Liaoning Key Laboratory of Food Biological Technology, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan 1, Ganjingzi District, Dalian 116034, ChinaLiaoning Key Laboratory of Food Biological Technology, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan 1, Ganjingzi District, Dalian 116034, ChinaLiaoning Key Laboratory of Food Biological Technology, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan 1, Ganjingzi District, Dalian 116034, ChinaLiaoning Key Laboratory of Food Biological Technology, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan 1, Ganjingzi District, Dalian 116034, ChinaLiaoning Key Laboratory of Food Biological Technology, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan 1, Ganjingzi District, Dalian 116034, ChinaLiaoning Key Laboratory of Food Biological Technology, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan 1, Ganjingzi District, Dalian 116034, ChinaLiaoning Key Laboratory of Food Biological Technology, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan 1, Ganjingzi District, Dalian 116034, ChinaFluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Transmission electron microscopy (TEM) demonstrated that the average particle sizes are 14 nm and 75 nm for starch and chitosan NSs, respectively. Fourier transform infrared (FT-IR) spectroscopy analysis showed that there are a large number of hydroxyl or amino groups on the surface of these polysaccharide-based NSs. Strong fluorescence with an excitation-dependent emission behaviour was observed under ultraviolet excitation. Interestingly, the photostability of the NSs was found to be superior to fluorescein and rhodamine B. The quantum yield of starch NSs could reach 11.12% under the excitation of 360 nm. The oxidative metal ions including Cu(II), Hg(II)and Fe(III) exhibited a quench effect on the fluorescence intensity of the prepared NSs. Both of the two kinds of the multicoloured NSs showed a maximum fluorescence intensity at pH 7, while the fluorescence intensity decreased dramatically when they were put in an either acidic or basic environment (at pH 3 or 11). The cytotoxicity study of starch NSs showed that low cell cytotoxicity and 80% viability was found after 24 h incubation, when their concentration was less than 10 mg/mL. The study also showed the possibility of using the multicoloured starch NSs for mouse melanoma cells and guppy fish imaging.http://www.mdpi.com/2079-4991/6/7/130polysaccharide nanostructuresfluorescent materialsquantum yieldcell imagingfish imaging |
| spellingShingle | Yinxue Zu Jingran Bi Huiping Yan Haitao Wang Yukun Song Bei-Wei Zhu Mingqian Tan Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging Nanomaterials polysaccharide nanostructures fluorescent materials quantum yield cell imaging fish imaging |
| title | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
| title_full | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
| title_fullStr | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
| title_full_unstemmed | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
| title_short | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
| title_sort | nanostructures derived from starch and chitosan for fluorescence bio imaging |
| topic | polysaccharide nanostructures fluorescent materials quantum yield cell imaging fish imaging |
| url | http://www.mdpi.com/2079-4991/6/7/130 |
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