Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa
In recent years, bats have been shown to host various novel bat-specific influenza viruses, including H17N10 and H18N11 in the Americas and the H9N2 subtype from Africa. <i>Rousettus aegyptiacus</i> (Egyptian Rousette bat) is recognized as a host species for diverse viral agents. This st...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/15/2/498 |
| _version_ | 1797617776001548288 |
|---|---|
| author | Rochelle Rademan Marike Geldenhuys Wanda Markotter |
| author_facet | Rochelle Rademan Marike Geldenhuys Wanda Markotter |
| author_sort | Rochelle Rademan |
| collection | DOAJ |
| description | In recent years, bats have been shown to host various novel bat-specific influenza viruses, including H17N10 and H18N11 in the Americas and the H9N2 subtype from Africa. <i>Rousettus aegyptiacus</i> (Egyptian Rousette bat) is recognized as a host species for diverse viral agents. This study focused on the molecular surveillance of a maternal colony in Limpopo, South Africa, between 2017–2018. A pan-influenza hemi-nested RT-PCR assay targeting the PB1 gene was established, and influenza A virus RNA was identified from one fecal sample out of 860 samples. Genome segments were recovered using segment-specific amplification combined with standard Sanger sequencing and Illumina unbiased sequencing. The identified influenza A virus was closely related to the H9N2 bat-influenza virus, confirming the circulation of this subtype among Egyptian fruit bat populations in Southern Africa. This bat H9N2 subtype contained amino acid residues associated with transmission and virulence in either mammalian or avian hosts, though it will likely require additional adaptations before spillover. |
| first_indexed | 2024-03-11T08:00:33Z |
| format | Article |
| id | doaj.art-3d49ebad7e5043cfa89981f26d699583 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T08:00:33Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3d49ebad7e5043cfa89981f26d6995832023-11-16T23:49:50ZengMDPI AGViruses1999-49152023-02-0115249810.3390/v15020498Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South AfricaRochelle Rademan0Marike Geldenhuys1Wanda Markotter2Centre for Viral Zoonoses, Department of Medical Virology, University of Pretoria, Pretoria 0001, South AfricaCentre for Viral Zoonoses, Department of Medical Virology, University of Pretoria, Pretoria 0001, South AfricaCentre for Viral Zoonoses, Department of Medical Virology, University of Pretoria, Pretoria 0001, South AfricaIn recent years, bats have been shown to host various novel bat-specific influenza viruses, including H17N10 and H18N11 in the Americas and the H9N2 subtype from Africa. <i>Rousettus aegyptiacus</i> (Egyptian Rousette bat) is recognized as a host species for diverse viral agents. This study focused on the molecular surveillance of a maternal colony in Limpopo, South Africa, between 2017–2018. A pan-influenza hemi-nested RT-PCR assay targeting the PB1 gene was established, and influenza A virus RNA was identified from one fecal sample out of 860 samples. Genome segments were recovered using segment-specific amplification combined with standard Sanger sequencing and Illumina unbiased sequencing. The identified influenza A virus was closely related to the H9N2 bat-influenza virus, confirming the circulation of this subtype among Egyptian fruit bat populations in Southern Africa. This bat H9N2 subtype contained amino acid residues associated with transmission and virulence in either mammalian or avian hosts, though it will likely require additional adaptations before spillover.https://www.mdpi.com/1999-4915/15/2/498influenza A virussurveillanceEgyptian Rousette batSouth AfricaH9N2 |
| spellingShingle | Rochelle Rademan Marike Geldenhuys Wanda Markotter Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa Viruses influenza A virus surveillance Egyptian Rousette bat South Africa H9N2 |
| title | Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa |
| title_full | Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa |
| title_fullStr | Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa |
| title_full_unstemmed | Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa |
| title_short | Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa |
| title_sort | detection and characterization of an h9n2 influenza a virus in the egyptian rousette bat in limpopo south africa |
| topic | influenza A virus surveillance Egyptian Rousette bat South Africa H9N2 |
| url | https://www.mdpi.com/1999-4915/15/2/498 |
| work_keys_str_mv | AT rochellerademan detectionandcharacterizationofanh9n2influenzaavirusintheegyptianrousettebatinlimpoposouthafrica AT marikegeldenhuys detectionandcharacterizationofanh9n2influenzaavirusintheegyptianrousettebatinlimpoposouthafrica AT wandamarkotter detectionandcharacterizationofanh9n2influenzaavirusintheegyptianrousettebatinlimpoposouthafrica |