DEVELOPMENT OF TEST KIT FOR DETECTION OF PANDEMIC STRAIN INFLUENZA VIRUS A (H1N1 2009) BY REAL TIME POLYMERASE CHAIN REACTION
Influenza viruses A play an important role in the structure of the incidence of people with acute respiratory viral infection, which make up 90% from all other infectious diseases. According to the World Health Organization, only severe flu worldwide suffer annually 3.5 million, of which 45–60% are...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2013-04-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/no2_2013/stepeniuk_2_2013.pdf |
| _version_ | 1797762644556382208 |
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| author | S. V. Stepaniuk M. I. Vudmaska S. L. Rybalko M. Y. Spivak |
| author_facet | S. V. Stepaniuk M. I. Vudmaska S. L. Rybalko M. Y. Spivak |
| author_sort | S. V. Stepaniuk |
| collection | DOAJ |
| description | Influenza viruses A play an important role in the structure of the incidence of people with acute respiratory viral infection, which make up 90% from all other infectious diseases. According to the World Health Organization, only severe flu worldwide suffer annually 3.5 million, of which 45–60% are children. An economic loss from seasonal flu epidemic is in average about 85% of economic losses from infectious diseases in general.
The experience of fighting the flu, accumulated over the years, has shown that to develop and deliver effective preventive measures necessary to build a system of permanent monitoring for influenza virus circulation, based on use of laboratory methods for accurate and rapid dentification and characterization of circulating strains of influenza virus A. Among the methods of laboratory diagnosis of influenza, the most effective is a method of polymerase chain reaction. Data on the evelopment of diagnostic test kits in the format of two-stage multiplex RTPCR-analysis for detection and genotyping of pandemic influenza virus A (H1N12009) are given. The results of laboratory and experimental research of «DIA Influenza H1N1» test system showed that it is effective and specific for detection of California pandemic influenza virus A (H1N12009) strains and can be used to diagnose disease caused by this strain of virus. Clinical trial of the course of the State registration by Ministry of Health of Ukraine have shown sensitivity and specificity of «DIA Influenza H1N1» test systems up to 100%. |
| first_indexed | 2024-03-12T19:31:23Z |
| format | Article |
| id | doaj.art-3d4bddb4ae5c45618a323db100ba08d9 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T19:31:23Z |
| publishDate | 2013-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-3d4bddb4ae5c45618a323db100ba08d92023-08-02T04:30:04ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2013-04-0162859110.15407/biotech6.02.085DEVELOPMENT OF TEST KIT FOR DETECTION OF PANDEMIC STRAIN INFLUENZA VIRUS A (H1N1 2009) BY REAL TIME POLYMERASE CHAIN REACTIONS. V. Stepaniuk0M. I. Vudmaska1S. L. Rybalko2M. Y. Spivak3PJSC SPC «DIAPROPH-MED», KyivPJSC SPC «DIAPROPH-MED», KyivInstitute of Epidemiology and Infectious diseases of Academy of Medical Sciences of Ukraine, KyivInstitute of Microbiology and Virology of National Academy of Sciences of Ukraine, KyivInfluenza viruses A play an important role in the structure of the incidence of people with acute respiratory viral infection, which make up 90% from all other infectious diseases. According to the World Health Organization, only severe flu worldwide suffer annually 3.5 million, of which 45–60% are children. An economic loss from seasonal flu epidemic is in average about 85% of economic losses from infectious diseases in general. The experience of fighting the flu, accumulated over the years, has shown that to develop and deliver effective preventive measures necessary to build a system of permanent monitoring for influenza virus circulation, based on use of laboratory methods for accurate and rapid dentification and characterization of circulating strains of influenza virus A. Among the methods of laboratory diagnosis of influenza, the most effective is a method of polymerase chain reaction. Data on the evelopment of diagnostic test kits in the format of two-stage multiplex RTPCR-analysis for detection and genotyping of pandemic influenza virus A (H1N12009) are given. The results of laboratory and experimental research of «DIA Influenza H1N1» test system showed that it is effective and specific for detection of California pandemic influenza virus A (H1N12009) strains and can be used to diagnose disease caused by this strain of virus. Clinical trial of the course of the State registration by Ministry of Health of Ukraine have shown sensitivity and specificity of «DIA Influenza H1N1» test systems up to 100%.http://biotechnology.kiev.ua/images/storage/no2_2013/stepeniuk_2_2013.pdfpandemic influenza virus A (H1N1)polymerase chain reaction in real timediagnostic test system |
| spellingShingle | S. V. Stepaniuk M. I. Vudmaska S. L. Rybalko M. Y. Spivak DEVELOPMENT OF TEST KIT FOR DETECTION OF PANDEMIC STRAIN INFLUENZA VIRUS A (H1N1 2009) BY REAL TIME POLYMERASE CHAIN REACTION Biotechnologia Acta pandemic influenza virus A (H1N1) polymerase chain reaction in real time diagnostic test system |
| title | DEVELOPMENT OF TEST KIT FOR DETECTION OF PANDEMIC STRAIN INFLUENZA VIRUS A (H1N1 2009) BY REAL TIME POLYMERASE CHAIN REACTION |
| title_full | DEVELOPMENT OF TEST KIT FOR DETECTION OF PANDEMIC STRAIN INFLUENZA VIRUS A (H1N1 2009) BY REAL TIME POLYMERASE CHAIN REACTION |
| title_fullStr | DEVELOPMENT OF TEST KIT FOR DETECTION OF PANDEMIC STRAIN INFLUENZA VIRUS A (H1N1 2009) BY REAL TIME POLYMERASE CHAIN REACTION |
| title_full_unstemmed | DEVELOPMENT OF TEST KIT FOR DETECTION OF PANDEMIC STRAIN INFLUENZA VIRUS A (H1N1 2009) BY REAL TIME POLYMERASE CHAIN REACTION |
| title_short | DEVELOPMENT OF TEST KIT FOR DETECTION OF PANDEMIC STRAIN INFLUENZA VIRUS A (H1N1 2009) BY REAL TIME POLYMERASE CHAIN REACTION |
| title_sort | development of test kit for detection of pandemic strain influenza virus a h1n1 2009 by real time polymerase chain reaction |
| topic | pandemic influenza virus A (H1N1) polymerase chain reaction in real time diagnostic test system |
| url | http://biotechnology.kiev.ua/images/storage/no2_2013/stepeniuk_2_2013.pdf |
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