The Role of Human Neural Stem Cell Secretomes on the Repair of Spinal Cord Injury Post-laminectomy in Rattus norvegicus Through the Analysis of Basso–Beattie–Bresnahan Score Locomotors, Interleukin-10, Matrix Metalloproteinase 9, and Transforming Growth Factor-β
Study Design Experimental animal study. Purpose This study aims to investigate the effects of treatment with human neural stem cell (HNSC) secretomes on subacute spinal cord injury (SCI) post-laminectomy by analyzing interleukin-10 (IL-10), matrix metalloproteinase 9 (MMP9), transforming growth fact...
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| Format: | Article |
| Language: | English |
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Korean Spine Society
2023-04-01
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| Series: | Asian Spine Journal |
| Subjects: | |
| Online Access: | http://www.asianspinejournal.org/upload/pdf/asj-2022-0152.pdf |
| _version_ | 1797835732587380736 |
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| author | I Nyoman Semita Dwikora Novembri Utomo Heri Suroto I Ketut Sudiana |
| author_facet | I Nyoman Semita Dwikora Novembri Utomo Heri Suroto I Ketut Sudiana |
| author_sort | I Nyoman Semita |
| collection | DOAJ |
| description | Study Design Experimental animal study. Purpose This study aims to investigate the effects of treatment with human neural stem cell (HNSC) secretomes on subacute spinal cord injury (SCI) post-laminectomy by analyzing interleukin-10 (IL-10), matrix metalloproteinase 9 (MMP9), transforming growth factor-β (TGF-β), and Basso–Beattie–Bresnahan (BBB) score locomotors as expressions of neurological recovery. Overview of Literature In the United States, SCI has a recovery rate of 0.08%, tetraplegia 58.7%, and paraplegia 40.6%. Therapeutic approaches to SCI have focused on modulating the secondary cascade to prevent neurological deterioration and glial scar formation. Increasing evidence has shown that the success of cell-based SCI therapy is attributed to the secretomes rather than the cells themselves, but the effect of treatment with HNSC secretomes in SCI is unclear. Methods This experimental study investigated 15 Rattus norvegicus rats that were divided into three groups: (1) normal, (2) SCI+non-secretome, and (3) SCI+secretome (30 μL, intrathecal Th10). Model subacute SCI post-laminectomy was performed in 60 seconds using an aneurysm Yasargil clip with a closing forceps weighing 65 g (150 kdyn). At 35 days post-injury, the specimens were collected, and the immunohistochemicals of IL-10, MMP9, and TGF-β were analyzed. Motor recovery was evaluated based on the BBB scores. Results The SCI post-laminectomy of rats treated with HNSC secretomes showed improvements in their locomotor recovery based on the BBB scores (p=0.000, mean=18.4) and decreased MMP9 (p=0.015) but had increased the levels of IL-10 (p=0.045) and TGF-β (p=0.01). Conclusions These results indicate that the factors associated with the HNSC secretomes can mitigate their pathophysiological processes of secondary damage after SCI and improve the locomotor functional outcomes in rats. |
| first_indexed | 2024-04-09T14:58:23Z |
| format | Article |
| id | doaj.art-3d546ae0260f4bdf9496d4da621f3308 |
| institution | Directory Open Access Journal |
| issn | 1976-1902 1976-7846 |
| language | English |
| last_indexed | 2024-04-09T14:58:23Z |
| publishDate | 2023-04-01 |
| publisher | Korean Spine Society |
| record_format | Article |
| series | Asian Spine Journal |
| spelling | doaj.art-3d546ae0260f4bdf9496d4da621f33082023-05-01T23:47:49ZengKorean Spine SocietyAsian Spine Journal1976-19021976-78462023-04-0117223123910.31616/asj.2022.01521493The Role of Human Neural Stem Cell Secretomes on the Repair of Spinal Cord Injury Post-laminectomy in Rattus norvegicus Through the Analysis of Basso–Beattie–Bresnahan Score Locomotors, Interleukin-10, Matrix Metalloproteinase 9, and Transforming Growth Factor-βI Nyoman Semita0Dwikora Novembri Utomo1Heri Suroto2I Ketut Sudiana3 Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia Orthopaedic Department, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia Orthopaedic Department, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia Department of Anatomic Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaStudy Design Experimental animal study. Purpose This study aims to investigate the effects of treatment with human neural stem cell (HNSC) secretomes on subacute spinal cord injury (SCI) post-laminectomy by analyzing interleukin-10 (IL-10), matrix metalloproteinase 9 (MMP9), transforming growth factor-β (TGF-β), and Basso–Beattie–Bresnahan (BBB) score locomotors as expressions of neurological recovery. Overview of Literature In the United States, SCI has a recovery rate of 0.08%, tetraplegia 58.7%, and paraplegia 40.6%. Therapeutic approaches to SCI have focused on modulating the secondary cascade to prevent neurological deterioration and glial scar formation. Increasing evidence has shown that the success of cell-based SCI therapy is attributed to the secretomes rather than the cells themselves, but the effect of treatment with HNSC secretomes in SCI is unclear. Methods This experimental study investigated 15 Rattus norvegicus rats that were divided into three groups: (1) normal, (2) SCI+non-secretome, and (3) SCI+secretome (30 μL, intrathecal Th10). Model subacute SCI post-laminectomy was performed in 60 seconds using an aneurysm Yasargil clip with a closing forceps weighing 65 g (150 kdyn). At 35 days post-injury, the specimens were collected, and the immunohistochemicals of IL-10, MMP9, and TGF-β were analyzed. Motor recovery was evaluated based on the BBB scores. Results The SCI post-laminectomy of rats treated with HNSC secretomes showed improvements in their locomotor recovery based on the BBB scores (p=0.000, mean=18.4) and decreased MMP9 (p=0.015) but had increased the levels of IL-10 (p=0.045) and TGF-β (p=0.01). Conclusions These results indicate that the factors associated with the HNSC secretomes can mitigate their pathophysiological processes of secondary damage after SCI and improve the locomotor functional outcomes in rats.http://www.asianspinejournal.org/upload/pdf/asj-2022-0152.pdfsecretomelocomotorsinterleukin-10matrix metalloproteinase 9transforming growth factor-β |
| spellingShingle | I Nyoman Semita Dwikora Novembri Utomo Heri Suroto I Ketut Sudiana The Role of Human Neural Stem Cell Secretomes on the Repair of Spinal Cord Injury Post-laminectomy in Rattus norvegicus Through the Analysis of Basso–Beattie–Bresnahan Score Locomotors, Interleukin-10, Matrix Metalloproteinase 9, and Transforming Growth Factor-β Asian Spine Journal secretome locomotors interleukin-10 matrix metalloproteinase 9 transforming growth factor-β |
| title | The Role of Human Neural Stem Cell Secretomes on the Repair of Spinal Cord Injury Post-laminectomy in Rattus norvegicus Through the Analysis of Basso–Beattie–Bresnahan Score Locomotors, Interleukin-10, Matrix Metalloproteinase 9, and Transforming Growth Factor-β |
| title_full | The Role of Human Neural Stem Cell Secretomes on the Repair of Spinal Cord Injury Post-laminectomy in Rattus norvegicus Through the Analysis of Basso–Beattie–Bresnahan Score Locomotors, Interleukin-10, Matrix Metalloproteinase 9, and Transforming Growth Factor-β |
| title_fullStr | The Role of Human Neural Stem Cell Secretomes on the Repair of Spinal Cord Injury Post-laminectomy in Rattus norvegicus Through the Analysis of Basso–Beattie–Bresnahan Score Locomotors, Interleukin-10, Matrix Metalloproteinase 9, and Transforming Growth Factor-β |
| title_full_unstemmed | The Role of Human Neural Stem Cell Secretomes on the Repair of Spinal Cord Injury Post-laminectomy in Rattus norvegicus Through the Analysis of Basso–Beattie–Bresnahan Score Locomotors, Interleukin-10, Matrix Metalloproteinase 9, and Transforming Growth Factor-β |
| title_short | The Role of Human Neural Stem Cell Secretomes on the Repair of Spinal Cord Injury Post-laminectomy in Rattus norvegicus Through the Analysis of Basso–Beattie–Bresnahan Score Locomotors, Interleukin-10, Matrix Metalloproteinase 9, and Transforming Growth Factor-β |
| title_sort | role of human neural stem cell secretomes on the repair of spinal cord injury post laminectomy in rattus norvegicus through the analysis of basso beattie bresnahan score locomotors interleukin 10 matrix metalloproteinase 9 and transforming growth factor β |
| topic | secretome locomotors interleukin-10 matrix metalloproteinase 9 transforming growth factor-β |
| url | http://www.asianspinejournal.org/upload/pdf/asj-2022-0152.pdf |
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