Automated identification of sequence-tailored Cas9 proteins using massive metagenomic data
Cas9 proteins require a target-adjacent sequence, the PAM, in order to cleave DNA. Here the authors develop a pipeline to accurately predict PAM sequences in order to facilitate the identification of Cas9s targeting specific sequences, including mutations.
| المؤلفون الرئيسيون: | , , , , , , , , , , , |
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| التنسيق: | مقال |
| اللغة: | English |
| منشور في: |
Nature Portfolio
2022-10-01
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| سلاسل: | Nature Communications |
| الوصول للمادة أونلاين: | https://doi.org/10.1038/s41467-022-34213-9 |