Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese Adolescents
Exosomes may contribute to the pathogenesis of obesity through their action as communication mediators. As we have previously demonstrated, in obese adolescents, some circulating miRNAs modified the C-type natriuretic peptide (<i>CNP</i>) expression and were associated with changes in me...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/139 |
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| author | Manuela Cabiati Emioli Randazzo Letizia Guiducci Alessandra Falleni Antonella Cecchettini Valentina Casieri Giovanni Federico Silvia Del Ry |
| author_facet | Manuela Cabiati Emioli Randazzo Letizia Guiducci Alessandra Falleni Antonella Cecchettini Valentina Casieri Giovanni Federico Silvia Del Ry |
| author_sort | Manuela Cabiati |
| collection | DOAJ |
| description | Exosomes may contribute to the pathogenesis of obesity through their action as communication mediators. As we have previously demonstrated, in obese adolescents, some circulating miRNAs modified the C-type natriuretic peptide (<i>CNP</i>) expression and were associated with changes in metabolic functions. At present no data are available on miRNA transport by exosomes in this condition. To verify and compare the presence and the expression of <i>CNP/NPR-B/NPR-C</i>, and some miRNAs (<i>miR-33a-3p</i>/<i>miR-223-5p</i>/<i>miR-142-5p</i>/<i>miRNA-4454</i>/<i>miRNA-181a-5p</i>/<i>miRNA-199-5p</i>), in circulating exosomes obtained from the same cohort of obese (O, n = 22) and normal-weight adolescents (N, n = 22). For the first time, we observed that exosomes carried <i>CNP</i> and its specific receptors only randomly both in O and N, suggesting that exosomes are not important carriers for the <i>CNP</i> system. On the contrary, exosomal miRNAs resulted ubiquitously and differentially expressed in O and N. O showed a significant decrease (<i>p</i> < 0.01) in the expression of all miRNAs except for <i>miR-4454</i> and <i>miR-142-5p</i>. We have found significant correlations among miRNAs themselves and with some inflammatory/metabolic factors of obesity. These relationships may help in finding new biomarkers, allowing us to recognize, at an early stage, obese children and adolescents at high risk to develop the disease complications in adult life. |
| first_indexed | 2024-03-11T09:59:43Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:59:43Z |
| publishDate | 2022-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3d59a227b3c6451eb85cfbca656ba4e52023-11-16T15:29:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124113910.3390/ijms24010139Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese AdolescentsManuela Cabiati0Emioli Randazzo1Letizia Guiducci2Alessandra Falleni3Antonella Cecchettini4Valentina Casieri5Giovanni Federico6Silvia Del Ry7Laboratory of Biochemistry and Molecular Biology, Institute of Clinical Physiology, CNR, 56124 Pisa, ItalyUnit of Pediatric Endocrinology and Diabetes, Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, ItalyLaboratory of Biochemistry and Molecular Biology, Institute of Clinical Physiology, CNR, 56124 Pisa, ItalyDepartment of Experimental and Clinical Medicine, University of Pisa, 56126 Pisa, ItalyLaboratory of Biochemistry and Molecular Biology, Institute of Clinical Physiology, CNR, 56124 Pisa, ItalyScuola Superiore Sant’Anna, Unit of Translational Critical Care Medicine, 56126 Pisa, ItalyUnit of Pediatric Endocrinology and Diabetes, Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, ItalyLaboratory of Biochemistry and Molecular Biology, Institute of Clinical Physiology, CNR, 56124 Pisa, ItalyExosomes may contribute to the pathogenesis of obesity through their action as communication mediators. As we have previously demonstrated, in obese adolescents, some circulating miRNAs modified the C-type natriuretic peptide (<i>CNP</i>) expression and were associated with changes in metabolic functions. At present no data are available on miRNA transport by exosomes in this condition. To verify and compare the presence and the expression of <i>CNP/NPR-B/NPR-C</i>, and some miRNAs (<i>miR-33a-3p</i>/<i>miR-223-5p</i>/<i>miR-142-5p</i>/<i>miRNA-4454</i>/<i>miRNA-181a-5p</i>/<i>miRNA-199-5p</i>), in circulating exosomes obtained from the same cohort of obese (O, n = 22) and normal-weight adolescents (N, n = 22). For the first time, we observed that exosomes carried <i>CNP</i> and its specific receptors only randomly both in O and N, suggesting that exosomes are not important carriers for the <i>CNP</i> system. On the contrary, exosomal miRNAs resulted ubiquitously and differentially expressed in O and N. O showed a significant decrease (<i>p</i> < 0.01) in the expression of all miRNAs except for <i>miR-4454</i> and <i>miR-142-5p</i>. We have found significant correlations among miRNAs themselves and with some inflammatory/metabolic factors of obesity. These relationships may help in finding new biomarkers, allowing us to recognize, at an early stage, obese children and adolescents at high risk to develop the disease complications in adult life.https://www.mdpi.com/1422-0067/24/1/139exosomesmiRNAobesitychildhoodReal-Time PCR |
| spellingShingle | Manuela Cabiati Emioli Randazzo Letizia Guiducci Alessandra Falleni Antonella Cecchettini Valentina Casieri Giovanni Federico Silvia Del Ry Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese Adolescents International Journal of Molecular Sciences exosomes miRNA obesity childhood Real-Time PCR |
| title | Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese Adolescents |
| title_full | Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese Adolescents |
| title_fullStr | Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese Adolescents |
| title_full_unstemmed | Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese Adolescents |
| title_short | Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese Adolescents |
| title_sort | evaluation of exosomal coding and non coding rna signature in obese adolescents |
| topic | exosomes miRNA obesity childhood Real-Time PCR |
| url | https://www.mdpi.com/1422-0067/24/1/139 |
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