Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiota
Abstract Two new bacterial strains, Marseille-P2698T (CSUR P2698 = DSM 103,121) and Marseille-P2260T (CSUR P2260 = DSM 101,844 = SN18), were isolated from human stools by the culturomic method. We used the taxonogenomic approach to fully describe these two new bacterial strains. The Marseille-P2698T...
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| Format: | Article |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-35443-7 |
| _version_ | 1797818052352409600 |
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| author | Afaf Hamame Reham Magdy Wasfy Cheikh Ibrahima Lo Florence Fenollar Didier Raoult Pierre-Edouard Fournier Linda Houhamdi |
| author_facet | Afaf Hamame Reham Magdy Wasfy Cheikh Ibrahima Lo Florence Fenollar Didier Raoult Pierre-Edouard Fournier Linda Houhamdi |
| author_sort | Afaf Hamame |
| collection | DOAJ |
| description | Abstract Two new bacterial strains, Marseille-P2698T (CSUR P2698 = DSM 103,121) and Marseille-P2260T (CSUR P2260 = DSM 101,844 = SN18), were isolated from human stools by the culturomic method. We used the taxonogenomic approach to fully describe these two new bacterial strains. The Marseille-P2698T strain was a Gram-negative, motile, non-spore-forming, rod-shaped bacterium. The Marseille-P2260T strain was a Gram-positive, motile, spore-forming rod-shaped bacterium. Major fatty acids found in Marseille-P2698T were C15:0 iso (63%), C15:0 anteiso (11%), and C17:0 3-OH iso (8%). Those found in Marseille-P2260T strain were C16:00 (39%), C18:1n9 (16%) and C18:1n7 (14%). Strains Marseille-P2698T and Marseille-P2260T had 16S rRNA gene sequence similarities of 91.50% with Odoribacter laneus T, and of 90.98% and 95.07% with Odoribacter splanchnicus T and Eubacterium sulci T, respectively. The exhibited digital DNA-DNA Hybridization values lower than 20.7%, and Orthologous Average Nucleotide Identity values lower than 73% compared to their closest related bacterial species O. splanchnicus T and E. sulci T respectively. Phenotypic, biochemical, phylogenetic, and genomic results obtained by comparative analyses provided sufficient evidence that both of the two studied strains Marseille-P2698T and Marseille-P2260T are two new bacterial species and new bacterial genera for which the names Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. were proposed, respectively. |
| first_indexed | 2024-03-13T09:02:27Z |
| format | Article |
| id | doaj.art-3d6092a725994115aafa438f5aeed1be |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T09:02:27Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3d6092a725994115aafa438f5aeed1be2023-05-28T11:14:04ZengNature PortfolioScientific Reports2045-23222023-05-0113111110.1038/s41598-023-35443-7Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiotaAfaf Hamame0Reham Magdy Wasfy1Cheikh Ibrahima Lo2Florence Fenollar3Didier Raoult4Pierre-Edouard Fournier5Linda Houhamdi6Aix Marseille Univ, IRD, AP-HM, MEPHIAix Marseille Univ, IRD, AP-HM, MEPHIIHU-Méditerranée InfectionIHU-Méditerranée InfectionAix Marseille Univ, IRD, AP-HM, MEPHIIHU-Méditerranée InfectionIHU-Méditerranée InfectionAbstract Two new bacterial strains, Marseille-P2698T (CSUR P2698 = DSM 103,121) and Marseille-P2260T (CSUR P2260 = DSM 101,844 = SN18), were isolated from human stools by the culturomic method. We used the taxonogenomic approach to fully describe these two new bacterial strains. The Marseille-P2698T strain was a Gram-negative, motile, non-spore-forming, rod-shaped bacterium. The Marseille-P2260T strain was a Gram-positive, motile, spore-forming rod-shaped bacterium. Major fatty acids found in Marseille-P2698T were C15:0 iso (63%), C15:0 anteiso (11%), and C17:0 3-OH iso (8%). Those found in Marseille-P2260T strain were C16:00 (39%), C18:1n9 (16%) and C18:1n7 (14%). Strains Marseille-P2698T and Marseille-P2260T had 16S rRNA gene sequence similarities of 91.50% with Odoribacter laneus T, and of 90.98% and 95.07% with Odoribacter splanchnicus T and Eubacterium sulci T, respectively. The exhibited digital DNA-DNA Hybridization values lower than 20.7%, and Orthologous Average Nucleotide Identity values lower than 73% compared to their closest related bacterial species O. splanchnicus T and E. sulci T respectively. Phenotypic, biochemical, phylogenetic, and genomic results obtained by comparative analyses provided sufficient evidence that both of the two studied strains Marseille-P2698T and Marseille-P2260T are two new bacterial species and new bacterial genera for which the names Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. were proposed, respectively.https://doi.org/10.1038/s41598-023-35443-7 |
| spellingShingle | Afaf Hamame Reham Magdy Wasfy Cheikh Ibrahima Lo Florence Fenollar Didier Raoult Pierre-Edouard Fournier Linda Houhamdi Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiota Scientific Reports |
| title | Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiota |
| title_full | Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiota |
| title_fullStr | Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiota |
| title_full_unstemmed | Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiota |
| title_short | Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiota |
| title_sort | taxonogenomics of culturomica massiliensis gen nov sp nov and emergencia timonensis gen nov sp nov new bacteria isolated from human stool microbiota |
| url | https://doi.org/10.1038/s41598-023-35443-7 |
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