Development of CdSe–ZnO Flower-Rod Core-Shell Structure Based Photoelectrochemical Biosensor for Detection of Norovirous RNA
In this work, the CdSe–ZnO flower-rod core-shell structure (CSZFRs) was prepared by ion-exchange method. The surface of CSZFRs was modified by 3-mercaptopropionic acid (MPA), and then the DNA probe was immobilized on the surface via chemical bond between -NH2 of DNA probe and -COOH of MPA....
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MDPI AG
2018-09-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/9/2980 |
| _version_ | 1798040042736713728 |
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| author | Zhizhong Han Qinghua Weng Chaofan Lin Jinquan Yi Jie Kang |
| author_facet | Zhizhong Han Qinghua Weng Chaofan Lin Jinquan Yi Jie Kang |
| author_sort | Zhizhong Han |
| collection | DOAJ |
| description | In this work, the CdSe–ZnO flower-rod core-shell structure (CSZFRs) was prepared by ion-exchange method. The surface of CSZFRs was modified by 3-mercaptopropionic acid (MPA), and then the DNA probe was immobilized on the surface via chemical bond between -NH2 of DNA probe and -COOH of MPA. Finally, the target norovirous (NV) RNA was combined with the probe according to the principle of complementary base pairing, resulting in a decrease of the photocurrent. The results show that the absorbance spectrum of visible light is enhanced for CSZFRs compared with pure ZnO. Under visible light irradiation, the photocurrent of CSZFRs is up to 0.1 mA, which can improve the sensitivity of the photoelectrochemical (PEC) biosensor. In the measurement range of 0–5.10 nM, the measured concentrations (c) have a good linear relationship with the output photocurrent of the biosensor. The linear regression equation is expressed as I = 0.03256 − 0.0033c (R2 = 0.99, S/N = 3) with a detection limit of 0.50 nM. Therefore, this work realizes a rapid and sensitive method for the detection of NV RNA. |
| first_indexed | 2024-04-11T22:02:03Z |
| format | Article |
| id | doaj.art-3d6108de622d42d49f52c2b06b49c38b |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:02:03Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-3d6108de622d42d49f52c2b06b49c38b2022-12-22T04:00:52ZengMDPI AGSensors1424-82202018-09-01189298010.3390/s18092980s18092980Development of CdSe–ZnO Flower-Rod Core-Shell Structure Based Photoelectrochemical Biosensor for Detection of Norovirous RNAZhizhong Han0Qinghua Weng1Chaofan Lin2Jinquan Yi3Jie Kang4School of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaSchool of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaSchool of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaSchool of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaSchool of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaIn this work, the CdSe–ZnO flower-rod core-shell structure (CSZFRs) was prepared by ion-exchange method. The surface of CSZFRs was modified by 3-mercaptopropionic acid (MPA), and then the DNA probe was immobilized on the surface via chemical bond between -NH2 of DNA probe and -COOH of MPA. Finally, the target norovirous (NV) RNA was combined with the probe according to the principle of complementary base pairing, resulting in a decrease of the photocurrent. The results show that the absorbance spectrum of visible light is enhanced for CSZFRs compared with pure ZnO. Under visible light irradiation, the photocurrent of CSZFRs is up to 0.1 mA, which can improve the sensitivity of the photoelectrochemical (PEC) biosensor. In the measurement range of 0–5.10 nM, the measured concentrations (c) have a good linear relationship with the output photocurrent of the biosensor. The linear regression equation is expressed as I = 0.03256 − 0.0033c (R2 = 0.99, S/N = 3) with a detection limit of 0.50 nM. Therefore, this work realizes a rapid and sensitive method for the detection of NV RNA.http://www.mdpi.com/1424-8220/18/9/2980CdSe–ZnO flower-rodphotoelectrochemical biosensornorovirous RNAion-exchange method |
| spellingShingle | Zhizhong Han Qinghua Weng Chaofan Lin Jinquan Yi Jie Kang Development of CdSe–ZnO Flower-Rod Core-Shell Structure Based Photoelectrochemical Biosensor for Detection of Norovirous RNA Sensors CdSe–ZnO flower-rod photoelectrochemical biosensor norovirous RNA ion-exchange method |
| title | Development of CdSe–ZnO Flower-Rod Core-Shell Structure Based Photoelectrochemical Biosensor for Detection of Norovirous RNA |
| title_full | Development of CdSe–ZnO Flower-Rod Core-Shell Structure Based Photoelectrochemical Biosensor for Detection of Norovirous RNA |
| title_fullStr | Development of CdSe–ZnO Flower-Rod Core-Shell Structure Based Photoelectrochemical Biosensor for Detection of Norovirous RNA |
| title_full_unstemmed | Development of CdSe–ZnO Flower-Rod Core-Shell Structure Based Photoelectrochemical Biosensor for Detection of Norovirous RNA |
| title_short | Development of CdSe–ZnO Flower-Rod Core-Shell Structure Based Photoelectrochemical Biosensor for Detection of Norovirous RNA |
| title_sort | development of cdse zno flower rod core shell structure based photoelectrochemical biosensor for detection of norovirous rna |
| topic | CdSe–ZnO flower-rod photoelectrochemical biosensor norovirous RNA ion-exchange method |
| url | http://www.mdpi.com/1424-8220/18/9/2980 |
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