Effects of astaxanthin in mice acutely infected with Trypanosoma cruzi
During Trypanosoma cruzi infection, oxidative stress is considered a contributing factor for dilated cardiomyopathy development. In this study, the effects of astaxanthin (ASTX) were evaluated as an alternative drug treatment for Chagas disease in a mouse model during the acute infection phase, give...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2017-01-01
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| Series: | Parasite |
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| Online Access: | https://doi.org/10.1051/parasite/2017018 |
| _version_ | 1797641197869596672 |
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| author | Contreras-Ortiz José María Eloy Barbabosa-Pliego Alberto Oros-Pantoja Rigoberto Aparicio-Burgos José Esteban Zepeda-Escobar José Antonio Hassan-Moustafa Wael Hegazy Ochoa-García Laucel Uxúa Alonso-Fresan María Tenorio Borroto Esvieta Vázquez-Chagoyán Juan Carlos |
| author_facet | Contreras-Ortiz José María Eloy Barbabosa-Pliego Alberto Oros-Pantoja Rigoberto Aparicio-Burgos José Esteban Zepeda-Escobar José Antonio Hassan-Moustafa Wael Hegazy Ochoa-García Laucel Uxúa Alonso-Fresan María Tenorio Borroto Esvieta Vázquez-Chagoyán Juan Carlos |
| author_sort | Contreras-Ortiz José María Eloy |
| collection | DOAJ |
| description | During Trypanosoma cruzi infection, oxidative stress is considered a contributing factor for dilated cardiomyopathy development. In this study, the effects of astaxanthin (ASTX) were evaluated as an alternative drug treatment for Chagas disease in a mouse model during the acute infection phase, given its anti-inflammatory, immunomodulating, and anti-oxidative properties. ASTX was tested in vitro in parasites grown axenically and in co-culture with Vero cells. In vivo tests were performed in BALB/c mice (4–6 weeks old) infected with Trypanosoma cruzi and supplemented with ASTX (10 mg/kg/day) and/or nifurtimox (NFMX; 100 mg/kg/day). Results show that ASTX has some detrimental effects on axenically cultured parasites, but not when cultured with mammalian cell monolayers. In vivo, ASTX did not have any therapeutic value against acute Trypanosoma cruzi infection, used either alone or in combination with NFMX. Infected animals treated with NFMX or ASTX/NFMX survived the experimental period (60 days), while infected animals treated only with ASTX died before day 30 post-infection. ASTX did not show any effect on the control of parasitemia; however, it was associated with an increment in focal heart lymphoplasmacytic infiltration, a reduced number of amastigote nests in cardiac tissue, and less hyperplasic spleen follicles when compared to control groups. Unexpectedly, ASTX showed a negative effect in infected animals co-treated with NFMX. An increment in parasitemia duration was observed, possibly due to ASTX blocking of free radicals, an anti-parasitic mechanism of NFMX. In conclusion, astaxanthin is not recommended during the acute phase of Chagas disease, either alone or in combination with nifurtimox. |
| first_indexed | 2024-03-11T13:41:11Z |
| format | Article |
| id | doaj.art-3d66f7330ad7409e8c958bfd3079bc5b |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-11T13:41:11Z |
| publishDate | 2017-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-3d66f7330ad7409e8c958bfd3079bc5b2023-11-02T11:38:17ZengEDP SciencesParasite1776-10422017-01-01241710.1051/parasite/2017018parasite170020Effects of astaxanthin in mice acutely infected with Trypanosoma cruziContreras-Ortiz José María EloyBarbabosa-Pliego AlbertoOros-Pantoja RigobertoAparicio-Burgos José EstebanZepeda-Escobar José AntonioHassan-Moustafa Wael HegazyOchoa-García LaucelUxúa Alonso-Fresan MaríaTenorio Borroto EsvietaVázquez-Chagoyán Juan CarlosDuring Trypanosoma cruzi infection, oxidative stress is considered a contributing factor for dilated cardiomyopathy development. In this study, the effects of astaxanthin (ASTX) were evaluated as an alternative drug treatment for Chagas disease in a mouse model during the acute infection phase, given its anti-inflammatory, immunomodulating, and anti-oxidative properties. ASTX was tested in vitro in parasites grown axenically and in co-culture with Vero cells. In vivo tests were performed in BALB/c mice (4–6 weeks old) infected with Trypanosoma cruzi and supplemented with ASTX (10 mg/kg/day) and/or nifurtimox (NFMX; 100 mg/kg/day). Results show that ASTX has some detrimental effects on axenically cultured parasites, but not when cultured with mammalian cell monolayers. In vivo, ASTX did not have any therapeutic value against acute Trypanosoma cruzi infection, used either alone or in combination with NFMX. Infected animals treated with NFMX or ASTX/NFMX survived the experimental period (60 days), while infected animals treated only with ASTX died before day 30 post-infection. ASTX did not show any effect on the control of parasitemia; however, it was associated with an increment in focal heart lymphoplasmacytic infiltration, a reduced number of amastigote nests in cardiac tissue, and less hyperplasic spleen follicles when compared to control groups. Unexpectedly, ASTX showed a negative effect in infected animals co-treated with NFMX. An increment in parasitemia duration was observed, possibly due to ASTX blocking of free radicals, an anti-parasitic mechanism of NFMX. In conclusion, astaxanthin is not recommended during the acute phase of Chagas disease, either alone or in combination with nifurtimox.https://doi.org/10.1051/parasite/2017018AstaxanthinChagas diseaseTrypanosoma cruziOxidative stressNifurtimox |
| spellingShingle | Contreras-Ortiz José María Eloy Barbabosa-Pliego Alberto Oros-Pantoja Rigoberto Aparicio-Burgos José Esteban Zepeda-Escobar José Antonio Hassan-Moustafa Wael Hegazy Ochoa-García Laucel Uxúa Alonso-Fresan María Tenorio Borroto Esvieta Vázquez-Chagoyán Juan Carlos Effects of astaxanthin in mice acutely infected with Trypanosoma cruzi Parasite Astaxanthin Chagas disease Trypanosoma cruzi Oxidative stress Nifurtimox |
| title | Effects of astaxanthin in mice acutely infected with Trypanosoma cruzi |
| title_full | Effects of astaxanthin in mice acutely infected with Trypanosoma cruzi |
| title_fullStr | Effects of astaxanthin in mice acutely infected with Trypanosoma cruzi |
| title_full_unstemmed | Effects of astaxanthin in mice acutely infected with Trypanosoma cruzi |
| title_short | Effects of astaxanthin in mice acutely infected with Trypanosoma cruzi |
| title_sort | effects of astaxanthin in mice acutely infected with trypanosoma cruzi |
| topic | Astaxanthin Chagas disease Trypanosoma cruzi Oxidative stress Nifurtimox |
| url | https://doi.org/10.1051/parasite/2017018 |
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