Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production
This study compared the cellular and genetic characteristics of bovine skeletal muscle satellite cells (SMSCs) from Hanwoo (a Korean native cattle breed), including calves and mature cattle. SMSCs were isolated using magnetic-activated cell sorting (MACS) from tissue samples of six Hanwoo (three cal...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927123001132 |
| _version_ | 1797394807984750592 |
|---|---|
| author | Bosung Kim Deunsol Ko Seong Ho Choi Sungkwon Park |
| author_facet | Bosung Kim Deunsol Ko Seong Ho Choi Sungkwon Park |
| author_sort | Bosung Kim |
| collection | DOAJ |
| description | This study compared the cellular and genetic characteristics of bovine skeletal muscle satellite cells (SMSCs) from Hanwoo (a Korean native cattle breed), including calves and mature cattle. SMSCs were isolated using magnetic-activated cell sorting (MACS) from tissue samples of six Hanwoo (three calves and three mature cattle) using the CD29 antibody. Calves’ SMSCs exhibited significantly faster growth rates than did those from cattle (P < 0.01), with a doubling time of 2.43 days. Genetic analysis revealed higher MyoD and Pax7 expression in SMSCs from calves during proliferation than in those from mature cattle (P < 0.001). However, FASN and PLAG1 expression levels were higher in mature cattle than in calves during both proliferation and differentiation (P < 0.001). These findings highlight the need for strategies to improve bovine muscle cell growth to produce competitive cultivated meat at a competitive price. |
| first_indexed | 2024-03-09T00:25:05Z |
| format | Article |
| id | doaj.art-3d68e9ee2f0247bea4424e967dfda5d3 |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-03-09T00:25:05Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-3d68e9ee2f0247bea4424e967dfda5d32023-12-12T04:35:54ZengElsevierCurrent Research in Food Science2665-92712023-01-017100545Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat productionBosung Kim0Deunsol Ko1Seong Ho Choi2Sungkwon Park3Sejong University, Department of Food Science and Biotechnology, Seoul, 05006, South KoreaSejong University, Department of Food Science and Biotechnology, Seoul, 05006, South KoreaChungbuk National University, Department of Animal Science, Cheongju, 28644, South KoreaSejong University, Department of Food Science and Biotechnology, Seoul, 05006, South Korea; Corresponding author. Department of Food Science and Biotechnology, Sejong University, 511 Yulgokwan, 209,Neungdong-ro, Gwangjin-gu, Seoul, South Korea.This study compared the cellular and genetic characteristics of bovine skeletal muscle satellite cells (SMSCs) from Hanwoo (a Korean native cattle breed), including calves and mature cattle. SMSCs were isolated using magnetic-activated cell sorting (MACS) from tissue samples of six Hanwoo (three calves and three mature cattle) using the CD29 antibody. Calves’ SMSCs exhibited significantly faster growth rates than did those from cattle (P < 0.01), with a doubling time of 2.43 days. Genetic analysis revealed higher MyoD and Pax7 expression in SMSCs from calves during proliferation than in those from mature cattle (P < 0.001). However, FASN and PLAG1 expression levels were higher in mature cattle than in calves during both proliferation and differentiation (P < 0.001). These findings highlight the need for strategies to improve bovine muscle cell growth to produce competitive cultivated meat at a competitive price.http://www.sciencedirect.com/science/article/pii/S2665927123001132Bovine muscle satellite cellPrimary cultureCell growthGenetic analysis |
| spellingShingle | Bosung Kim Deunsol Ko Seong Ho Choi Sungkwon Park Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production Current Research in Food Science Bovine muscle satellite cell Primary culture Cell growth Genetic analysis |
| title | Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production |
| title_full | Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production |
| title_fullStr | Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production |
| title_full_unstemmed | Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production |
| title_short | Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production |
| title_sort | bovine muscle satellite cells in calves and cattle a comparative study of cellular and genetic characteristics for cultivated meat production |
| topic | Bovine muscle satellite cell Primary culture Cell growth Genetic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2665927123001132 |
| work_keys_str_mv | AT bosungkim bovinemusclesatellitecellsincalvesandcattleacomparativestudyofcellularandgeneticcharacteristicsforcultivatedmeatproduction AT deunsolko bovinemusclesatellitecellsincalvesandcattleacomparativestudyofcellularandgeneticcharacteristicsforcultivatedmeatproduction AT seonghochoi bovinemusclesatellitecellsincalvesandcattleacomparativestudyofcellularandgeneticcharacteristicsforcultivatedmeatproduction AT sungkwonpark bovinemusclesatellitecellsincalvesandcattleacomparativestudyofcellularandgeneticcharacteristicsforcultivatedmeatproduction |