Polymerization of chondroitin sulfate and its stimulatory effect on cartilage regeneration; a bioactive material for cartilage regeneration
Chondroitin sulfate (CS) is one of the major glycosaminoglycans (GAGs). GAGs are linear polymers comprising disaccharide residues and are found as the side chains of proteoglycans. CS has significant stimulatory effects on cell behavior and is widely used in tissue-engineered and drug delivery devic...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Polymer Testing |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0142941822003178 |
| _version_ | 1797996686173274112 |
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| author | N. Rashidi M. Tamaddon C. Liu J. Czernuszka |
| author_facet | N. Rashidi M. Tamaddon C. Liu J. Czernuszka |
| author_sort | N. Rashidi |
| collection | DOAJ |
| description | Chondroitin sulfate (CS) is one of the major glycosaminoglycans (GAGs). GAGs are linear polymers comprising disaccharide residues and are found as the side chains of proteoglycans. CS has significant stimulatory effects on cell behavior and is widely used in tissue-engineered and drug delivery devices. However, it is difficult to incorporate a sufficient amount of CS into biopolymer-based scaffolds such as collagen to take full advantage of its benefit. In this study, CS has been polymerized to an 11 times higher molecular weight polymer (PCS) in an attempt to overcome this deficiency. We have previously shown that PCS was significantly more effective than CS in chondrogenesis. This study aimed to characterize the physicochemical properties of the manufactured PCS. PCS was characterized by Fourier transform infra-red (FTIR) spectroscopy together with X-ray photoelectron spectroscopy (XPS) to obtain information about its chemical structure and elemental composition. Its molecular size was measured using dynamic light scattering (DLS) and its viscoelastic properties were determined by rheology measurements. The average PCS diameter increased 5 times by polymerization and PCS has significantly enhanced viscoelastic properties compared to CS. The molecular weight of PCS was calculated from the rheological experiment to give more than an order of magnitude increase over CS molecular weight. Based on these results, we believe there is a great potential for using PCS in regenerative medicine devices. |
| first_indexed | 2024-04-11T10:21:16Z |
| format | Article |
| id | doaj.art-3d6be856180b4d73b8bf6e58d3a20e42 |
| institution | Directory Open Access Journal |
| issn | 0142-9418 |
| language | English |
| last_indexed | 2024-04-11T10:21:16Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Polymer Testing |
| spelling | doaj.art-3d6be856180b4d73b8bf6e58d3a20e422022-12-22T04:29:44ZengElsevierPolymer Testing0142-94182022-12-01116107796Polymerization of chondroitin sulfate and its stimulatory effect on cartilage regeneration; a bioactive material for cartilage regenerationN. Rashidi0M. Tamaddon1C. Liu2J. Czernuszka3Department of Materials, University of Oxford, Oxford, OX1 3PH, UK; Corresponding author.Institute of Orthopaedic & Musculoskeletal Science, Division of Surgery & Interventional Science, University College London, Royal National Orthopaedic Hospital, Stanmore, HA7 4LP, UKInstitute of Orthopaedic & Musculoskeletal Science, Division of Surgery & Interventional Science, University College London, Royal National Orthopaedic Hospital, Stanmore, HA7 4LP, UKDepartment of Materials, University of Oxford, Oxford, OX1 3PH, UKChondroitin sulfate (CS) is one of the major glycosaminoglycans (GAGs). GAGs are linear polymers comprising disaccharide residues and are found as the side chains of proteoglycans. CS has significant stimulatory effects on cell behavior and is widely used in tissue-engineered and drug delivery devices. However, it is difficult to incorporate a sufficient amount of CS into biopolymer-based scaffolds such as collagen to take full advantage of its benefit. In this study, CS has been polymerized to an 11 times higher molecular weight polymer (PCS) in an attempt to overcome this deficiency. We have previously shown that PCS was significantly more effective than CS in chondrogenesis. This study aimed to characterize the physicochemical properties of the manufactured PCS. PCS was characterized by Fourier transform infra-red (FTIR) spectroscopy together with X-ray photoelectron spectroscopy (XPS) to obtain information about its chemical structure and elemental composition. Its molecular size was measured using dynamic light scattering (DLS) and its viscoelastic properties were determined by rheology measurements. The average PCS diameter increased 5 times by polymerization and PCS has significantly enhanced viscoelastic properties compared to CS. The molecular weight of PCS was calculated from the rheological experiment to give more than an order of magnitude increase over CS molecular weight. Based on these results, we believe there is a great potential for using PCS in regenerative medicine devices.http://www.sciencedirect.com/science/article/pii/S0142941822003178Chondroitin sulfate polymerizationCartilage regenerationCollagen II -Chondroitin sulfate scaffoldsHigher molecular weight chondroitin sulfateCartilage tissue engineering |
| spellingShingle | N. Rashidi M. Tamaddon C. Liu J. Czernuszka Polymerization of chondroitin sulfate and its stimulatory effect on cartilage regeneration; a bioactive material for cartilage regeneration Polymer Testing Chondroitin sulfate polymerization Cartilage regeneration Collagen II -Chondroitin sulfate scaffolds Higher molecular weight chondroitin sulfate Cartilage tissue engineering |
| title | Polymerization of chondroitin sulfate and its stimulatory effect on cartilage regeneration; a bioactive material for cartilage regeneration |
| title_full | Polymerization of chondroitin sulfate and its stimulatory effect on cartilage regeneration; a bioactive material for cartilage regeneration |
| title_fullStr | Polymerization of chondroitin sulfate and its stimulatory effect on cartilage regeneration; a bioactive material for cartilage regeneration |
| title_full_unstemmed | Polymerization of chondroitin sulfate and its stimulatory effect on cartilage regeneration; a bioactive material for cartilage regeneration |
| title_short | Polymerization of chondroitin sulfate and its stimulatory effect on cartilage regeneration; a bioactive material for cartilage regeneration |
| title_sort | polymerization of chondroitin sulfate and its stimulatory effect on cartilage regeneration a bioactive material for cartilage regeneration |
| topic | Chondroitin sulfate polymerization Cartilage regeneration Collagen II -Chondroitin sulfate scaffolds Higher molecular weight chondroitin sulfate Cartilage tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S0142941822003178 |
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