Comparative Analysis of Transcriptome and sRNAs Expression Patterns in the <i>Brachypodium distachyon</i>—<i>Magnaporthe oryzae</i> Pathosystems
The hemibiotrophic fungus <i>Magnaporthe oryzae</i> (Mo) is the causative agent of rice blast and can infect aerial and root tissues of a variety of Poaceae, including the model <i>Brachypodium distachyon</i> (Bd). To gain insight in gene regulation processes occurring at ear...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/650 |
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| author | Silvia Zanini Ena Šečić Tobias Busche Matteo Galli Ying Zheng Jörn Kalinowski Karl-Heinz Kogel |
| author_facet | Silvia Zanini Ena Šečić Tobias Busche Matteo Galli Ying Zheng Jörn Kalinowski Karl-Heinz Kogel |
| author_sort | Silvia Zanini |
| collection | DOAJ |
| description | The hemibiotrophic fungus <i>Magnaporthe oryzae</i> (Mo) is the causative agent of rice blast and can infect aerial and root tissues of a variety of Poaceae, including the model <i>Brachypodium distachyon</i> (Bd). To gain insight in gene regulation processes occurring at early disease stages, we comparatively analyzed fungal and plant mRNA and sRNA expression in leaves and roots. A total of 310 Mo genes were detected consistently and differentially expressed in both leaves and roots. Contrary to Mo, only minor overlaps were observed in plant differentially expressed genes (DEGs), with 233 Bd-DEGs in infected leaves at 2 days post inoculation (DPI), compared to 4978 at 4 DPI, and 138 in infected roots. sRNA sequencing revealed a broad spectrum of Mo-sRNAs that accumulated in infected tissues, including candidates predicted to target Bd mRNAs. Conversely, we identified a subset of potential Bd-sRNAs directed against fungal cell wall components, virulence genes and transcription factors. We also show a requirement of operable RNAi genes from the DICER-like (DCL) and ARGONAUTE (AGO) families for fungal virulence. Overall, our work elucidates the extensive reprogramming of transcriptomes and sRNAs in both plant host (Bd) and fungal pathogen (Mo), further corroborating the critical role played by sRNA species in the establishment of the interaction and its outcome. |
| first_indexed | 2024-03-09T05:13:49Z |
| format | Article |
| id | doaj.art-3d6cecb76b81420f96cd096911344d47 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:13:49Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3d6cecb76b81420f96cd096911344d472023-12-03T12:46:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122265010.3390/ijms22020650Comparative Analysis of Transcriptome and sRNAs Expression Patterns in the <i>Brachypodium distachyon</i>—<i>Magnaporthe oryzae</i> PathosystemsSilvia Zanini0Ena Šečić1Tobias Busche2Matteo Galli3Ying Zheng4Jörn Kalinowski5Karl-Heinz Kogel6Institute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University (iFZ), 35392 Giessen, GermanyInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University (iFZ), 35392 Giessen, GermanyCenter for Biotechnology (CeBiTec), Bielefeld University, 33615 Bielefeld, GermanyInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University (iFZ), 35392 Giessen, GermanyInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University (iFZ), 35392 Giessen, GermanyCenter for Biotechnology (CeBiTec), Bielefeld University, 33615 Bielefeld, GermanyInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University (iFZ), 35392 Giessen, GermanyThe hemibiotrophic fungus <i>Magnaporthe oryzae</i> (Mo) is the causative agent of rice blast and can infect aerial and root tissues of a variety of Poaceae, including the model <i>Brachypodium distachyon</i> (Bd). To gain insight in gene regulation processes occurring at early disease stages, we comparatively analyzed fungal and plant mRNA and sRNA expression in leaves and roots. A total of 310 Mo genes were detected consistently and differentially expressed in both leaves and roots. Contrary to Mo, only minor overlaps were observed in plant differentially expressed genes (DEGs), with 233 Bd-DEGs in infected leaves at 2 days post inoculation (DPI), compared to 4978 at 4 DPI, and 138 in infected roots. sRNA sequencing revealed a broad spectrum of Mo-sRNAs that accumulated in infected tissues, including candidates predicted to target Bd mRNAs. Conversely, we identified a subset of potential Bd-sRNAs directed against fungal cell wall components, virulence genes and transcription factors. We also show a requirement of operable RNAi genes from the DICER-like (DCL) and ARGONAUTE (AGO) families for fungal virulence. Overall, our work elucidates the extensive reprogramming of transcriptomes and sRNAs in both plant host (Bd) and fungal pathogen (Mo), further corroborating the critical role played by sRNA species in the establishment of the interaction and its outcome.https://www.mdpi.com/1422-0067/22/2/650argonautedicerRNAiplant diseasesmall RNAvirulence |
| spellingShingle | Silvia Zanini Ena Šečić Tobias Busche Matteo Galli Ying Zheng Jörn Kalinowski Karl-Heinz Kogel Comparative Analysis of Transcriptome and sRNAs Expression Patterns in the <i>Brachypodium distachyon</i>—<i>Magnaporthe oryzae</i> Pathosystems International Journal of Molecular Sciences argonaute dicer RNAi plant disease small RNA virulence |
| title | Comparative Analysis of Transcriptome and sRNAs Expression Patterns in the <i>Brachypodium distachyon</i>—<i>Magnaporthe oryzae</i> Pathosystems |
| title_full | Comparative Analysis of Transcriptome and sRNAs Expression Patterns in the <i>Brachypodium distachyon</i>—<i>Magnaporthe oryzae</i> Pathosystems |
| title_fullStr | Comparative Analysis of Transcriptome and sRNAs Expression Patterns in the <i>Brachypodium distachyon</i>—<i>Magnaporthe oryzae</i> Pathosystems |
| title_full_unstemmed | Comparative Analysis of Transcriptome and sRNAs Expression Patterns in the <i>Brachypodium distachyon</i>—<i>Magnaporthe oryzae</i> Pathosystems |
| title_short | Comparative Analysis of Transcriptome and sRNAs Expression Patterns in the <i>Brachypodium distachyon</i>—<i>Magnaporthe oryzae</i> Pathosystems |
| title_sort | comparative analysis of transcriptome and srnas expression patterns in the i brachypodium distachyon i i magnaporthe oryzae i pathosystems |
| topic | argonaute dicer RNAi plant disease small RNA virulence |
| url | https://www.mdpi.com/1422-0067/22/2/650 |
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