Experimental Evidence of the Long-Term Survival of Infective African Swine Fever Virus Strain Ba71V in Soil under Different Conditions
The survival of African swine fever virus (ASFV) on different matrices and its infectivity in wild as well as domestic swine is still a matter of interest. ASFV is resistant to environmental effects; this fact is enhanced by the presence of organic material. Therefore, the aim of this work was to de...
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MDPI AG
2022-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/6/648 |
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| author | Jana Prodelalova Lenka Kavanova Jiri Salat Romana Moutelikova Sarka Kobzova Magdalena Krasna Petra Vasickova Bronislav Simek Petr Vaclavek |
| author_facet | Jana Prodelalova Lenka Kavanova Jiri Salat Romana Moutelikova Sarka Kobzova Magdalena Krasna Petra Vasickova Bronislav Simek Petr Vaclavek |
| author_sort | Jana Prodelalova |
| collection | DOAJ |
| description | The survival of African swine fever virus (ASFV) on different matrices and its infectivity in wild as well as domestic swine is still a matter of interest. ASFV is resistant to environmental effects; this fact is enhanced by the presence of organic material. Therefore, the aim of this work was to determine the ability of laboratory ASFV to survive in soil at different temperatures (4 and 22 °C) and with and without the presence of blood using culture procedures. The suitability of the procedure for determining the viability and titre of the ASFV field strain by the hemadsorption method was also verified, when a higher decrease in virus infectivity in the case of clay compared with peat was demonstrated. The stability of the virus was clearly temperature-dependent, the infectious virus was detected after 112 days, and the viral DNA was still detected in the matrix 210 days after inoculation in a relatively high and stable concentration (between 10<sup>6</sup> and 10<sup>7</sup> genome equivalents/mL). Based on this knowledge, soil and other environmental samples could provide rapid and reliable information on the disease outbreak and serve as indicators of the risk posed by the affected locality. |
| first_indexed | 2024-03-09T22:48:16Z |
| format | Article |
| id | doaj.art-3d6d90a6f5b14263b6d43d1346151ef2 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T22:48:16Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-3d6d90a6f5b14263b6d43d1346151ef22023-11-23T18:24:39ZengMDPI AGPathogens2076-08172022-06-0111664810.3390/pathogens11060648Experimental Evidence of the Long-Term Survival of Infective African Swine Fever Virus Strain Ba71V in Soil under Different ConditionsJana Prodelalova0Lenka Kavanova1Jiri Salat2Romana Moutelikova3Sarka Kobzova4Magdalena Krasna5Petra Vasickova6Bronislav Simek7Petr Vaclavek8Veterinary Research Institute, Hudcova 70, 602 00 Brno, Czech RepublicVeterinary Research Institute, Hudcova 70, 602 00 Brno, Czech RepublicVeterinary Research Institute, Hudcova 70, 602 00 Brno, Czech RepublicVeterinary Research Institute, Hudcova 70, 602 00 Brno, Czech RepublicVeterinary Research Institute, Hudcova 70, 602 00 Brno, Czech RepublicVeterinary Research Institute, Hudcova 70, 602 00 Brno, Czech RepublicVeterinary Research Institute, Hudcova 70, 602 00 Brno, Czech RepublicState Veterinary Institute Jihlava, Rantirovska 20, 586 01 Jihlava, Czech RepublicState Veterinary Institute Jihlava, Rantirovska 20, 586 01 Jihlava, Czech RepublicThe survival of African swine fever virus (ASFV) on different matrices and its infectivity in wild as well as domestic swine is still a matter of interest. ASFV is resistant to environmental effects; this fact is enhanced by the presence of organic material. Therefore, the aim of this work was to determine the ability of laboratory ASFV to survive in soil at different temperatures (4 and 22 °C) and with and without the presence of blood using culture procedures. The suitability of the procedure for determining the viability and titre of the ASFV field strain by the hemadsorption method was also verified, when a higher decrease in virus infectivity in the case of clay compared with peat was demonstrated. The stability of the virus was clearly temperature-dependent, the infectious virus was detected after 112 days, and the viral DNA was still detected in the matrix 210 days after inoculation in a relatively high and stable concentration (between 10<sup>6</sup> and 10<sup>7</sup> genome equivalents/mL). Based on this knowledge, soil and other environmental samples could provide rapid and reliable information on the disease outbreak and serve as indicators of the risk posed by the affected locality.https://www.mdpi.com/2076-0817/11/6/648ASFVfield isolatesstabilityhemadsorptioninfectivity testing |
| spellingShingle | Jana Prodelalova Lenka Kavanova Jiri Salat Romana Moutelikova Sarka Kobzova Magdalena Krasna Petra Vasickova Bronislav Simek Petr Vaclavek Experimental Evidence of the Long-Term Survival of Infective African Swine Fever Virus Strain Ba71V in Soil under Different Conditions Pathogens ASFV field isolates stability hemadsorption infectivity testing |
| title | Experimental Evidence of the Long-Term Survival of Infective African Swine Fever Virus Strain Ba71V in Soil under Different Conditions |
| title_full | Experimental Evidence of the Long-Term Survival of Infective African Swine Fever Virus Strain Ba71V in Soil under Different Conditions |
| title_fullStr | Experimental Evidence of the Long-Term Survival of Infective African Swine Fever Virus Strain Ba71V in Soil under Different Conditions |
| title_full_unstemmed | Experimental Evidence of the Long-Term Survival of Infective African Swine Fever Virus Strain Ba71V in Soil under Different Conditions |
| title_short | Experimental Evidence of the Long-Term Survival of Infective African Swine Fever Virus Strain Ba71V in Soil under Different Conditions |
| title_sort | experimental evidence of the long term survival of infective african swine fever virus strain ba71v in soil under different conditions |
| topic | ASFV field isolates stability hemadsorption infectivity testing |
| url | https://www.mdpi.com/2076-0817/11/6/648 |
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