ThASR3 confers salt and osmotic stress tolerances in transgenic Tamarix and Arabidopsis
Abstract Background ASR (abscisic acid-, stress-, and ripening-induced) gene family plays a crucial role in responding to abiotic stresses in plants. However, the roles of ASR genes protecting plants against high salt and drought stresses remain unknown in Tamarix hispida. Results In this study, a s...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03942-w |
| _version_ | 1797980213830746112 |
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| author | Yu Zhang Huijun Ma Tianchang Zhou Zhenyu Zhu Yue Zhang Xin Zhao Chao Wang |
| author_facet | Yu Zhang Huijun Ma Tianchang Zhou Zhenyu Zhu Yue Zhang Xin Zhao Chao Wang |
| author_sort | Yu Zhang |
| collection | DOAJ |
| description | Abstract Background ASR (abscisic acid-, stress-, and ripening-induced) gene family plays a crucial role in responding to abiotic stresses in plants. However, the roles of ASR genes protecting plants against high salt and drought stresses remain unknown in Tamarix hispida. Results In this study, a salt and drought-induced ASR gene, ThASR3, was isolated from Tamarix hispida. Transgenic Arabidopsis overexpressing ThASR3 exhibited stimulating root growth and increasing fresh weight compared with wild-type (WT) plants under both salt and water deficit stresses. To further analyze the gain- and loss-of-function of ThASR3, the transgenic T. hispida plants overexpressing or RNA interference (RNAi)-silencing ThASR3 were generated using transient transformation. The overexpression of ThASR3 in Tamarix and Arabidopsis plants displayed enhanced reactive oxygen species (ROS) scavenging capability under high salt and osmotic stress conditions, including increasing the activities of antioxidant enzymes and the contents of proline and betaine, and reducing malondialdehyde (MDA) content and electrolyte leakage rates. Conclusion Our results indicate that ThASR3 functions as a positive regulator in Tamarix responses to salt and osmotic stresses and confers multiple abiotic stress tolerances in transgenic plants, which may have an important application value in the genetic improvement of forest tree resistance. |
| first_indexed | 2024-04-11T05:52:03Z |
| format | Article |
| id | doaj.art-3d7229d555d44c2ca6a1eb9dc29c1675 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-11T05:52:03Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-3d7229d555d44c2ca6a1eb9dc29c16752022-12-22T04:42:03ZengBMCBMC Plant Biology1471-22292022-12-0122111310.1186/s12870-022-03942-wThASR3 confers salt and osmotic stress tolerances in transgenic Tamarix and ArabidopsisYu Zhang0Huijun Ma1Tianchang Zhou2Zhenyu Zhu3Yue Zhang4Xin Zhao5Chao Wang6State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityAbstract Background ASR (abscisic acid-, stress-, and ripening-induced) gene family plays a crucial role in responding to abiotic stresses in plants. However, the roles of ASR genes protecting plants against high salt and drought stresses remain unknown in Tamarix hispida. Results In this study, a salt and drought-induced ASR gene, ThASR3, was isolated from Tamarix hispida. Transgenic Arabidopsis overexpressing ThASR3 exhibited stimulating root growth and increasing fresh weight compared with wild-type (WT) plants under both salt and water deficit stresses. To further analyze the gain- and loss-of-function of ThASR3, the transgenic T. hispida plants overexpressing or RNA interference (RNAi)-silencing ThASR3 were generated using transient transformation. The overexpression of ThASR3 in Tamarix and Arabidopsis plants displayed enhanced reactive oxygen species (ROS) scavenging capability under high salt and osmotic stress conditions, including increasing the activities of antioxidant enzymes and the contents of proline and betaine, and reducing malondialdehyde (MDA) content and electrolyte leakage rates. Conclusion Our results indicate that ThASR3 functions as a positive regulator in Tamarix responses to salt and osmotic stresses and confers multiple abiotic stress tolerances in transgenic plants, which may have an important application value in the genetic improvement of forest tree resistance.https://doi.org/10.1186/s12870-022-03942-wAbiotic stressASR proteinGene expressionROS-scavenging capabilityTamarix hispida |
| spellingShingle | Yu Zhang Huijun Ma Tianchang Zhou Zhenyu Zhu Yue Zhang Xin Zhao Chao Wang ThASR3 confers salt and osmotic stress tolerances in transgenic Tamarix and Arabidopsis BMC Plant Biology Abiotic stress ASR protein Gene expression ROS-scavenging capability Tamarix hispida |
| title | ThASR3 confers salt and osmotic stress tolerances in transgenic Tamarix and Arabidopsis |
| title_full | ThASR3 confers salt and osmotic stress tolerances in transgenic Tamarix and Arabidopsis |
| title_fullStr | ThASR3 confers salt and osmotic stress tolerances in transgenic Tamarix and Arabidopsis |
| title_full_unstemmed | ThASR3 confers salt and osmotic stress tolerances in transgenic Tamarix and Arabidopsis |
| title_short | ThASR3 confers salt and osmotic stress tolerances in transgenic Tamarix and Arabidopsis |
| title_sort | thasr3 confers salt and osmotic stress tolerances in transgenic tamarix and arabidopsis |
| topic | Abiotic stress ASR protein Gene expression ROS-scavenging capability Tamarix hispida |
| url | https://doi.org/10.1186/s12870-022-03942-w |
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