Protocol for immunodot blot detection of UVB photoproducts in extracellular DNA

Protocol for immunodot blot detection of UVB photoproducts in extracellular DNA

Summary: UV radiation induces the formation of adducts in genomic DNA within cells that are later found to be present in cell-free fractions associated with extracellular vesicles (EVs) outside of cells. Here, we present a protocol for isolating UV photoproducts in extracellular DNA released from UV...

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Bibliographic Details
Main Authors:	M. Alexandra Carpenter, Sri Yerrapragada, Aleena Alex, Michael G. Kemp
Format:	Article
Language:	English
Published:	Elsevier 2024-03-01
Series:	STAR Protocols
Subjects:	Cell culture Cell isolation Cell-based Assays Cell separation/fractionation Antibody Signal Transduction
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724000030

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