Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique

Background: Toxocara cati is considered as one of the main etiological agents of toxocariasis with global and regional importance. As there is no information on proteomics of T. cati, herein, we reported the results obtained by proteomic analysis of somatic proteins extract, using a mass spectrometr...

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Main Authors: Nooshinmehr SOLEYMANI, Ruth Birner GRUNBERGER, khalil ABNOUS, Hassan BORJI, Faezeh VAHDATI
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2021-02-01
Series:Iranian Journal of Parasitology
Subjects:
Online Access:https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2720
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author Nooshinmehr SOLEYMANI
Ruth Birner GRUNBERGER
khalil ABNOUS
Hassan BORJI
Faezeh VAHDATI
author_facet Nooshinmehr SOLEYMANI
Ruth Birner GRUNBERGER
khalil ABNOUS
Hassan BORJI
Faezeh VAHDATI
author_sort Nooshinmehr SOLEYMANI
collection DOAJ
description Background: Toxocara cati is considered as one of the main etiological agents of toxocariasis with global and regional importance. As there is no information on proteomics of T. cati, herein, we reported the results obtained by proteomic analysis of somatic proteins extract, using a mass spectrometry (LC–MS/MS) approach. Methods: Somatic extract fractions were separated by two-dimensional SDS-PAGE and were electro blotted on to PVDF membranes for immunoblot analysis, then collected the immunogenic spots which response of antibodies of the paratenic hosts (mice) to the antigens ( Mashhad, 2017), and analyzed by LC–MS/MS. The LC-MS/MS data were analyzed by Mascot database, Taxonomy Toxocara, and common contaminants, in Omics Center, Biotechnology Medical University of Graz (Austria, 2018). Result: The protein spots were isolated between 15–140 kDa ranges using 3–10 non-linear IPG strips and Brilliant Blue Coomassie. Ten proteins were characterized as immunogenic proteins, seven of them were identified and three of them were unknown proteins. Conclusion: This study provided additional information about the somatic antigens of T. cati, which can lead to the development of new strategies for novel immuno-modulators, drug targets, subunit vaccines and immunodiagnostic kits for toxocariasis.
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spelling doaj.art-3d743e98ae794f008e5d09fb5f80c5be2022-12-21T21:58:09ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2021-02-0116123312720Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics TechniqueNooshinmehr SOLEYMANI0Ruth Birner GRUNBERGER1khalil ABNOUS2Hassan BORJI3Faezeh VAHDATI41. Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran2. Austrian Center of Industrial Biotechnology, Graz, Austria 3. Diagnostic and Research Institute of Pathology, Medical University of Graz, Graz, Austria 4. Omics Center Graz, Bio Tech Med-Graz, Graz, Austria 5. Institute of Chemical Technologies and Analytics, Vienna, Austria6. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mash-had, Iran1. Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran6. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mash-had, IranBackground: Toxocara cati is considered as one of the main etiological agents of toxocariasis with global and regional importance. As there is no information on proteomics of T. cati, herein, we reported the results obtained by proteomic analysis of somatic proteins extract, using a mass spectrometry (LC–MS/MS) approach. Methods: Somatic extract fractions were separated by two-dimensional SDS-PAGE and were electro blotted on to PVDF membranes for immunoblot analysis, then collected the immunogenic spots which response of antibodies of the paratenic hosts (mice) to the antigens ( Mashhad, 2017), and analyzed by LC–MS/MS. The LC-MS/MS data were analyzed by Mascot database, Taxonomy Toxocara, and common contaminants, in Omics Center, Biotechnology Medical University of Graz (Austria, 2018). Result: The protein spots were isolated between 15–140 kDa ranges using 3–10 non-linear IPG strips and Brilliant Blue Coomassie. Ten proteins were characterized as immunogenic proteins, seven of them were identified and three of them were unknown proteins. Conclusion: This study provided additional information about the somatic antigens of T. cati, which can lead to the development of new strategies for novel immuno-modulators, drug targets, subunit vaccines and immunodiagnostic kits for toxocariasis.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2720toxocariasismass spectrometrysomatic extractimmunoblot
spellingShingle Nooshinmehr SOLEYMANI
Ruth Birner GRUNBERGER
khalil ABNOUS
Hassan BORJI
Faezeh VAHDATI
Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique
Iranian Journal of Parasitology
toxocariasis
mass spectrometry
somatic extract
immunoblot
title Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique
title_full Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique
title_fullStr Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique
title_full_unstemmed Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique
title_short Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique
title_sort identification and immunological characterization of somatic proteins from adults of toxocara cati by proteomics technique
topic toxocariasis
mass spectrometry
somatic extract
immunoblot
url https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2720
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