Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique
Background: Toxocara cati is considered as one of the main etiological agents of toxocariasis with global and regional importance. As there is no information on proteomics of T. cati, herein, we reported the results obtained by proteomic analysis of somatic proteins extract, using a mass spectrometr...
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Format: | Article |
Language: | English |
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Tehran University of Medical Sciences
2021-02-01
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Series: | Iranian Journal of Parasitology |
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Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2720 |
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author | Nooshinmehr SOLEYMANI Ruth Birner GRUNBERGER khalil ABNOUS Hassan BORJI Faezeh VAHDATI |
author_facet | Nooshinmehr SOLEYMANI Ruth Birner GRUNBERGER khalil ABNOUS Hassan BORJI Faezeh VAHDATI |
author_sort | Nooshinmehr SOLEYMANI |
collection | DOAJ |
description | Background: Toxocara cati is considered as one of the main etiological agents of toxocariasis with global and regional importance. As there is no information on proteomics of T. cati, herein, we reported the results obtained by proteomic analysis of somatic proteins extract, using a mass spectrometry (LC–MS/MS) approach.
Methods: Somatic extract fractions were separated by two-dimensional SDS-PAGE and were electro blotted on to PVDF membranes for immunoblot analysis, then collected the immunogenic spots which response of antibodies of the paratenic hosts (mice) to the antigens ( Mashhad, 2017), and analyzed by LC–MS/MS. The LC-MS/MS data were analyzed by Mascot database, Taxonomy Toxocara, and common contaminants, in Omics Center, Biotechnology Medical University of Graz (Austria, 2018).
Result: The protein spots were isolated between 15–140 kDa ranges using 3–10 non-linear IPG strips and Brilliant Blue Coomassie. Ten proteins were characterized as immunogenic proteins, seven of them were identified and three of them were unknown proteins.
Conclusion: This study provided additional information about the somatic antigens of T. cati, which can lead to the development of new strategies for novel immuno-modulators, drug targets, subunit vaccines and immunodiagnostic kits for toxocariasis. |
first_indexed | 2024-12-17T07:40:19Z |
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institution | Directory Open Access Journal |
issn | 1735-7020 2008-238X |
language | English |
last_indexed | 2024-12-17T07:40:19Z |
publishDate | 2021-02-01 |
publisher | Tehran University of Medical Sciences |
record_format | Article |
series | Iranian Journal of Parasitology |
spelling | doaj.art-3d743e98ae794f008e5d09fb5f80c5be2022-12-21T21:58:09ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2021-02-0116123312720Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics TechniqueNooshinmehr SOLEYMANI0Ruth Birner GRUNBERGER1khalil ABNOUS2Hassan BORJI3Faezeh VAHDATI41. Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran2. Austrian Center of Industrial Biotechnology, Graz, Austria 3. Diagnostic and Research Institute of Pathology, Medical University of Graz, Graz, Austria 4. Omics Center Graz, Bio Tech Med-Graz, Graz, Austria 5. Institute of Chemical Technologies and Analytics, Vienna, Austria6. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mash-had, Iran1. Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran6. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mash-had, IranBackground: Toxocara cati is considered as one of the main etiological agents of toxocariasis with global and regional importance. As there is no information on proteomics of T. cati, herein, we reported the results obtained by proteomic analysis of somatic proteins extract, using a mass spectrometry (LC–MS/MS) approach. Methods: Somatic extract fractions were separated by two-dimensional SDS-PAGE and were electro blotted on to PVDF membranes for immunoblot analysis, then collected the immunogenic spots which response of antibodies of the paratenic hosts (mice) to the antigens ( Mashhad, 2017), and analyzed by LC–MS/MS. The LC-MS/MS data were analyzed by Mascot database, Taxonomy Toxocara, and common contaminants, in Omics Center, Biotechnology Medical University of Graz (Austria, 2018). Result: The protein spots were isolated between 15–140 kDa ranges using 3–10 non-linear IPG strips and Brilliant Blue Coomassie. Ten proteins were characterized as immunogenic proteins, seven of them were identified and three of them were unknown proteins. Conclusion: This study provided additional information about the somatic antigens of T. cati, which can lead to the development of new strategies for novel immuno-modulators, drug targets, subunit vaccines and immunodiagnostic kits for toxocariasis.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2720toxocariasismass spectrometrysomatic extractimmunoblot |
spellingShingle | Nooshinmehr SOLEYMANI Ruth Birner GRUNBERGER khalil ABNOUS Hassan BORJI Faezeh VAHDATI Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique Iranian Journal of Parasitology toxocariasis mass spectrometry somatic extract immunoblot |
title | Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique |
title_full | Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique |
title_fullStr | Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique |
title_full_unstemmed | Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique |
title_short | Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique |
title_sort | identification and immunological characterization of somatic proteins from adults of toxocara cati by proteomics technique |
topic | toxocariasis mass spectrometry somatic extract immunoblot |
url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2720 |
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