Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in <i>Akebia trifoliata</i> (Thunb.) Koidz Seeds
<i>Akebia trifoliata</i> (Thunb.) Koidz is an important Chinese medicinal and economic crop. Its seeds, which are rich in fatty acids, are usually discarded. As of now, <i>A. trifoliata</i> lipid biosynthesis pathways and genes have not been clearly described. In this work, w...
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2022-06-01
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| author | Yicheng Zhong Yunlei Zhao Yue Wang Juan Niu Zhimin Sun Jianhua Chen Mingbao Luan |
| author_facet | Yicheng Zhong Yunlei Zhao Yue Wang Juan Niu Zhimin Sun Jianhua Chen Mingbao Luan |
| author_sort | Yicheng Zhong |
| collection | DOAJ |
| description | <i>Akebia trifoliata</i> (Thunb.) Koidz is an important Chinese medicinal and economic crop. Its seeds, which are rich in fatty acids, are usually discarded. As of now, <i>A. trifoliata</i> lipid biosynthesis pathways and genes have not been clearly described. In this work, we found that seed and fruit development of <i>A. trifoliata</i> were not synchronized, and that when the fruit was ripe, seed oil content was not at its highest. As seeds developed, linoleic and oleic acid content was found to decrease and increase, respectively. RNA sequencing yielded 108.45 GB of clean reads from 15 cDNA libraries, containing 8756 differentially expressed genes. We identified 65 unigenes associated with lipid biosynthesis, including fatty acid and triacylglycerol biosynthesis. The 65 unigenes were mapped to the <i>A. trifoliata</i> lipid synthesis pathway. There were 20 <i>AtrFAD</i> family members in <i>A. trifoliata</i>, which could be divided into four sub-groups with the highest number of <i>AtrSADs</i>. Our study revealed the dynamic changes in <i>A. trifoliata</i> seed oil content and composition during its growth period and provides large-scale and comprehensive transcriptome data of <i>A. trifoliata</i> seeds. These findings provide a basis for the improvement of <i>A. trifoliata</i> seed oil yield and quality. |
| first_indexed | 2024-03-10T00:22:37Z |
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| spelling | doaj.art-3d76eed0c8994497a6dec143e63104e12023-11-23T15:39:24ZengMDPI AGBiology2079-77372022-06-0111685510.3390/biology11060855Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in <i>Akebia trifoliata</i> (Thunb.) Koidz SeedsYicheng Zhong0Yunlei Zhao1Yue Wang2Juan Niu3Zhimin Sun4Jianhua Chen5Mingbao Luan6Key Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaCotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaKey Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaKey Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaKey Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaKey Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaKey Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China<i>Akebia trifoliata</i> (Thunb.) Koidz is an important Chinese medicinal and economic crop. Its seeds, which are rich in fatty acids, are usually discarded. As of now, <i>A. trifoliata</i> lipid biosynthesis pathways and genes have not been clearly described. In this work, we found that seed and fruit development of <i>A. trifoliata</i> were not synchronized, and that when the fruit was ripe, seed oil content was not at its highest. As seeds developed, linoleic and oleic acid content was found to decrease and increase, respectively. RNA sequencing yielded 108.45 GB of clean reads from 15 cDNA libraries, containing 8756 differentially expressed genes. We identified 65 unigenes associated with lipid biosynthesis, including fatty acid and triacylglycerol biosynthesis. The 65 unigenes were mapped to the <i>A. trifoliata</i> lipid synthesis pathway. There were 20 <i>AtrFAD</i> family members in <i>A. trifoliata</i>, which could be divided into four sub-groups with the highest number of <i>AtrSADs</i>. Our study revealed the dynamic changes in <i>A. trifoliata</i> seed oil content and composition during its growth period and provides large-scale and comprehensive transcriptome data of <i>A. trifoliata</i> seeds. These findings provide a basis for the improvement of <i>A. trifoliata</i> seed oil yield and quality.https://www.mdpi.com/2079-7737/11/6/855<i>Akebia trifoliata</i>seed oilfatty acid biosynthesis |
| spellingShingle | Yicheng Zhong Yunlei Zhao Yue Wang Juan Niu Zhimin Sun Jianhua Chen Mingbao Luan Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in <i>Akebia trifoliata</i> (Thunb.) Koidz Seeds Biology <i>Akebia trifoliata</i> seed oil fatty acid biosynthesis |
| title | Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in <i>Akebia trifoliata</i> (Thunb.) Koidz Seeds |
| title_full | Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in <i>Akebia trifoliata</i> (Thunb.) Koidz Seeds |
| title_fullStr | Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in <i>Akebia trifoliata</i> (Thunb.) Koidz Seeds |
| title_full_unstemmed | Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in <i>Akebia trifoliata</i> (Thunb.) Koidz Seeds |
| title_short | Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in <i>Akebia trifoliata</i> (Thunb.) Koidz Seeds |
| title_sort | transcriptome analysis and gc ms profiling of key fatty acid biosynthesis genes in i akebia trifoliata i thunb koidz seeds |
| topic | <i>Akebia trifoliata</i> seed oil fatty acid biosynthesis |
| url | https://www.mdpi.com/2079-7737/11/6/855 |
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