Microglia in Gemistocytic Astrocytomas
Background: Gemistocytic astrocytomas behave more aggressively than other diffusely growing astrocytic tumours. They are characterised by a high mutation rate of the p53 gene and cytological as well as immunological abnormalities including frequent perivascular mononuclear infiltrates. Microglia are...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2005-03-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/2979 |
| _version_ | 1818947630782218240 |
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| author | F Geranmayeh BW Scheithauer MB Graeber |
| author_facet | F Geranmayeh BW Scheithauer MB Graeber |
| author_sort | F Geranmayeh |
| collection | DOAJ |
| description | Background: Gemistocytic astrocytomas behave more aggressively than other diffusely growing astrocytic tumours. They are characterised by a high mutation rate of the p53 gene and cytological as well as immunological abnormalities including frequent perivascular mononuclear infiltrates. Microglia are resident brain macrophage precursor cells that form a network of immune competent cells within the normal central nervous system. They are of increasing interest in the context of glioma growth. Material and Methods: We selected 23 tumour samples from among 201 samples obtained from patients with gemistocytic astrocytomas operated on at the Mayo Clinic between 1985 and 1998. These tumours have been previously analysed for p53 mutations, p53 protein and proliferative activity (Neurosurgery 48:187-194, 2001). Immunolabelling for three established microglial markers, CR3/43, Ki-M1P and iba1 was carried out on adjacent tissue sections. In addition radioactive in-situ hybridization was carried out. Results: A high number of microglial cells were detected in gemistocytic astrocytomas. More microglia were present when the fraction of gemistocytes was high (correlation coefficient= 0.740, P< .0000542). Varying numbers of gemistocytic tumour cells expressed MHC class II molecules. Importantly, the more class II immunoreactive gemistocytes were present, the fewer class II positive microglial cells could be detected (correlation coefficient= -0.623, P |
| first_indexed | 2024-12-20T08:33:58Z |
| format | Article |
| id | doaj.art-3d797c43f1ce49588d5465c453ad14dd |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-20T08:33:58Z |
| publishDate | 2005-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-3d797c43f1ce49588d5465c453ad14dd2022-12-21T19:46:36ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932005-03-0134Supple 1Microglia in Gemistocytic Astrocytomas F Geranmayeh0 BW Scheithauer1 MB Graeber2 Background: Gemistocytic astrocytomas behave more aggressively than other diffusely growing astrocytic tumours. They are characterised by a high mutation rate of the p53 gene and cytological as well as immunological abnormalities including frequent perivascular mononuclear infiltrates. Microglia are resident brain macrophage precursor cells that form a network of immune competent cells within the normal central nervous system. They are of increasing interest in the context of glioma growth. Material and Methods: We selected 23 tumour samples from among 201 samples obtained from patients with gemistocytic astrocytomas operated on at the Mayo Clinic between 1985 and 1998. These tumours have been previously analysed for p53 mutations, p53 protein and proliferative activity (Neurosurgery 48:187-194, 2001). Immunolabelling for three established microglial markers, CR3/43, Ki-M1P and iba1 was carried out on adjacent tissue sections. In addition radioactive in-situ hybridization was carried out. Results: A high number of microglial cells were detected in gemistocytic astrocytomas. More microglia were present when the fraction of gemistocytes was high (correlation coefficient= 0.740, P< .0000542). Varying numbers of gemistocytic tumour cells expressed MHC class II molecules. Importantly, the more class II immunoreactive gemistocytes were present, the fewer class II positive microglial cells could be detected (correlation coefficient= -0.623, Phttps://ijph.tums.ac.ir/index.php/ijph/article/view/2979MicrogliaGemistocytic astrocytomas |
| spellingShingle | F Geranmayeh BW Scheithauer MB Graeber Microglia in Gemistocytic Astrocytomas Iranian Journal of Public Health Microglia Gemistocytic astrocytomas |
| title | Microglia in Gemistocytic Astrocytomas |
| title_full | Microglia in Gemistocytic Astrocytomas |
| title_fullStr | Microglia in Gemistocytic Astrocytomas |
| title_full_unstemmed | Microglia in Gemistocytic Astrocytomas |
| title_short | Microglia in Gemistocytic Astrocytomas |
| title_sort | microglia in gemistocytic astrocytomas |
| topic | Microglia Gemistocytic astrocytomas |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/2979 |
| work_keys_str_mv | AT fgeranmayeh microgliaingemistocyticastrocytomas AT bwscheithauer microgliaingemistocyticastrocytomas AT mbgraeber microgliaingemistocyticastrocytomas |