Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers
Fescue toxicosis impacts beef cattle production via reductions in weight gain and muscle development. Isoflavone supplementation has displayed potential for mitigating these effects. The objective of the current study was to evaluate isoflavone supplementation with fescue seed consumption on rumen a...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6651/12/12/744 |
| _version_ | 1797546693635342336 |
|---|---|
| author | Taylor B. Ault-Seay Emily A. Melchior-Tiffany Brooke A. Clemmons Juan F. Cordero Gary E. Bates Michael D. Flythe James L. Klotz Huihua Ji Jack P. Goodman Kyle J. McLean Phillip R. Myer |
| author_facet | Taylor B. Ault-Seay Emily A. Melchior-Tiffany Brooke A. Clemmons Juan F. Cordero Gary E. Bates Michael D. Flythe James L. Klotz Huihua Ji Jack P. Goodman Kyle J. McLean Phillip R. Myer |
| author_sort | Taylor B. Ault-Seay |
| collection | DOAJ |
| description | Fescue toxicosis impacts beef cattle production via reductions in weight gain and muscle development. Isoflavone supplementation has displayed potential for mitigating these effects. The objective of the current study was to evaluate isoflavone supplementation with fescue seed consumption on rumen and serum metabolomes. Angus steers (<i>n</i> = 36) were allocated randomly in a 2 × 2 factorial arrangement of treatments including endophyte-infected (E+) or endophyte-free (E−) tall fescue seed, with (P+) or without (P−) isoflavones. Steers were provided a basal diet with fescue seed for 21 days, while isoflavones were orally administered daily. Following the trial, blood and rumen fluid were collected for metabolite analysis. Metabolites were extracted and then analyzed by UPLC-MS. The MAVEN program was implemented to identify metabolites for MetaboAnalyst 4.0 and SAS 9.4 statistical analysis. Seven differentially abundant metabolites were identified in serum by isoflavone treatment, and eleven metabolites in the rumen due to seed type (<i>p</i> < 0.05). Pathways affected by treatments were related to amino acid and nucleic acid metabolism in both rumen fluid and serum (<i>p</i> < 0.05). Therefore, metabolism was altered by fescue seed in the rumen; however, isoflavones altered metabolism systemically to potentially mitigate detrimental effects of seed and improve animal performance. |
| first_indexed | 2024-03-10T14:32:52Z |
| format | Article |
| id | doaj.art-3d7a30b9b32f45b4984b284f3e69d022 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T14:32:52Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-3d7a30b9b32f45b4984b284f3e69d0222023-11-20T22:25:40ZengMDPI AGToxins2072-66512020-11-01121274410.3390/toxins12120744Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef SteersTaylor B. Ault-Seay0Emily A. Melchior-Tiffany1Brooke A. Clemmons2Juan F. Cordero3Gary E. Bates4Michael D. Flythe5James L. Klotz6Huihua Ji7Jack P. Goodman8Kyle J. McLean9Phillip R. Myer10Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USADepartment of Animal Science, University of Tennessee, Knoxville, TN 37996, USADepartment of Animal Science, University of Tennessee, Knoxville, TN 37996, USADepartment of Animal Science, University of Tennessee, Knoxville, TN 37996, USADepartment of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USAUSDA-ARS, Forage-Animal Production Research Unit, Lexington, KY 40546, USAUSDA-ARS, Forage-Animal Production Research Unit, Lexington, KY 40546, USAKentucky Tobacco Research and Development Center, University of Kentucky, Lexington, KY 40546, USADepartment of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USADepartment of Animal Science, University of Tennessee, Knoxville, TN 37996, USADepartment of Animal Science, University of Tennessee, Knoxville, TN 37996, USAFescue toxicosis impacts beef cattle production via reductions in weight gain and muscle development. Isoflavone supplementation has displayed potential for mitigating these effects. The objective of the current study was to evaluate isoflavone supplementation with fescue seed consumption on rumen and serum metabolomes. Angus steers (<i>n</i> = 36) were allocated randomly in a 2 × 2 factorial arrangement of treatments including endophyte-infected (E+) or endophyte-free (E−) tall fescue seed, with (P+) or without (P−) isoflavones. Steers were provided a basal diet with fescue seed for 21 days, while isoflavones were orally administered daily. Following the trial, blood and rumen fluid were collected for metabolite analysis. Metabolites were extracted and then analyzed by UPLC-MS. The MAVEN program was implemented to identify metabolites for MetaboAnalyst 4.0 and SAS 9.4 statistical analysis. Seven differentially abundant metabolites were identified in serum by isoflavone treatment, and eleven metabolites in the rumen due to seed type (<i>p</i> < 0.05). Pathways affected by treatments were related to amino acid and nucleic acid metabolism in both rumen fluid and serum (<i>p</i> < 0.05). Therefore, metabolism was altered by fescue seed in the rumen; however, isoflavones altered metabolism systemically to potentially mitigate detrimental effects of seed and improve animal performance.https://www.mdpi.com/2072-6651/12/12/744beef cattleendophyteergot alkaloidfescue toxicosisisoflavonemetabolites |
| spellingShingle | Taylor B. Ault-Seay Emily A. Melchior-Tiffany Brooke A. Clemmons Juan F. Cordero Gary E. Bates Michael D. Flythe James L. Klotz Huihua Ji Jack P. Goodman Kyle J. McLean Phillip R. Myer Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers Toxins beef cattle endophyte ergot alkaloid fescue toxicosis isoflavone metabolites |
| title | Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers |
| title_full | Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers |
| title_fullStr | Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers |
| title_full_unstemmed | Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers |
| title_short | Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers |
| title_sort | rumen and serum metabolomes in response to endophyte infected tall fescue seed and isoflavone supplementation in beef steers |
| topic | beef cattle endophyte ergot alkaloid fescue toxicosis isoflavone metabolites |
| url | https://www.mdpi.com/2072-6651/12/12/744 |
| work_keys_str_mv | AT taylorbaultseay rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT emilyamelchiortiffany rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT brookeaclemmons rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT juanfcordero rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT garyebates rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT michaeldflythe rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT jameslklotz rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT huihuaji rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT jackpgoodman rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT kylejmclean rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers AT philliprmyer rumenandserummetabolomesinresponsetoendophyteinfectedtallfescueseedandisoflavonesupplementationinbeefsteers |