Active MMP-8 Quantitative Test as an Adjunctive Tool for Early Diagnosis of Periodontitis
Periodontitis is a host-mediated bacterial disease that affects the tooth attachment apparatus. Metalloproteinase-8 (MMP-8), a validated biomarker, could aid in clinical diagnosis. This study aimed to evaluate the diagnostic performance of active (a) MMP-8 immunotest versus total (t) MMP-8 ELISA for...
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MDPI AG
2021-08-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/8/1503 |
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| author | Marcela Hernández Mauricio Baeza Ismo T. Räisänen Johanna Contreras Taina Tervahartiala Alejandra Chaparro Timo Sorsa Patricia Hernández-Ríos |
| author_facet | Marcela Hernández Mauricio Baeza Ismo T. Räisänen Johanna Contreras Taina Tervahartiala Alejandra Chaparro Timo Sorsa Patricia Hernández-Ríos |
| author_sort | Marcela Hernández |
| collection | DOAJ |
| description | Periodontitis is a host-mediated bacterial disease that affects the tooth attachment apparatus. Metalloproteinase-8 (MMP-8), a validated biomarker, could aid in clinical diagnosis. This study aimed to evaluate the diagnostic performance of active (a) MMP-8 immunotest versus total (t) MMP-8 ELISA for quantitative real-time diagnosis and assessment of periodontitis severity at the site level. Gingival crevicular fluid (GCF) was sampled from 30 healthy, 42 mild, and 59 severe periodontitis sites from thirty-one volunteers. MMP-8 concentrations were determined by time-resolved immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using the STATA package. Both active and total MMP-8-based methods discriminated among sites according to periodontal diagnosis and severity, with a positive correlation between the two tests (<i>p</i> < 0.001). (a) MMP-8 models showed the best performance in receiver operating characteristic (ROC) curves to discriminate between healthy and periodontitis sites (area under the curve [AUC] = 0.89), while (t) MMP-8 demonstrated a high diagnostic precision in the detection of mild from severe periodontitis sites (AUC ≥ 0.80). The use of (a) MMP-8 and (t) MMP-8 could represent a useful adjunctive tool for periodontitis diagnosis and severity. These results support the applicability of new point-of-care methods in the monitoring of high-risk periodontal patients. |
| first_indexed | 2024-03-10T08:52:28Z |
| format | Article |
| id | doaj.art-3d7ab7ba62a64116bd9dc9caaf8d0f24 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T08:52:28Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-3d7ab7ba62a64116bd9dc9caaf8d0f242023-11-22T07:21:26ZengMDPI AGDiagnostics2075-44182021-08-01118150310.3390/diagnostics11081503Active MMP-8 Quantitative Test as an Adjunctive Tool for Early Diagnosis of PeriodontitisMarcela Hernández0Mauricio Baeza1Ismo T. Räisänen2Johanna Contreras3Taina Tervahartiala4Alejandra Chaparro5Timo Sorsa6Patricia Hernández-Ríos7Laboratory of Periodontal Biology, Department of Oral Pathology and Medicine, Faculty of Dentistry, University of Chile, Santiago 8380544, ChileDepartment of Conservative Dentistry, Faculty of Dentistry, University of Chile, Santiago 8380544, ChileDepartment of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, FinlandDepartment of Conservative Dentistry, Faculty of Dentistry, University of Chile, Santiago 8380544, ChileDepartment of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, FinlandDepartment of Periodontology, Centro de Investigación e Innovación Biomédica (CIIB), Faculty of Dentistry, Universidad de Los Andes, Santiago 7620001, ChileDepartment of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, FinlandDepartment of Conservative Dentistry, Faculty of Dentistry, University of Chile, Santiago 8380544, ChilePeriodontitis is a host-mediated bacterial disease that affects the tooth attachment apparatus. Metalloproteinase-8 (MMP-8), a validated biomarker, could aid in clinical diagnosis. This study aimed to evaluate the diagnostic performance of active (a) MMP-8 immunotest versus total (t) MMP-8 ELISA for quantitative real-time diagnosis and assessment of periodontitis severity at the site level. Gingival crevicular fluid (GCF) was sampled from 30 healthy, 42 mild, and 59 severe periodontitis sites from thirty-one volunteers. MMP-8 concentrations were determined by time-resolved immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using the STATA package. Both active and total MMP-8-based methods discriminated among sites according to periodontal diagnosis and severity, with a positive correlation between the two tests (<i>p</i> < 0.001). (a) MMP-8 models showed the best performance in receiver operating characteristic (ROC) curves to discriminate between healthy and periodontitis sites (area under the curve [AUC] = 0.89), while (t) MMP-8 demonstrated a high diagnostic precision in the detection of mild from severe periodontitis sites (AUC ≥ 0.80). The use of (a) MMP-8 and (t) MMP-8 could represent a useful adjunctive tool for periodontitis diagnosis and severity. These results support the applicability of new point-of-care methods in the monitoring of high-risk periodontal patients.https://www.mdpi.com/2075-4418/11/8/1503periodontitisperiodontal diseasematrix metalloproteinase-8gingival crevicular fluidbiomarkersdiagnosis |
| spellingShingle | Marcela Hernández Mauricio Baeza Ismo T. Räisänen Johanna Contreras Taina Tervahartiala Alejandra Chaparro Timo Sorsa Patricia Hernández-Ríos Active MMP-8 Quantitative Test as an Adjunctive Tool for Early Diagnosis of Periodontitis Diagnostics periodontitis periodontal disease matrix metalloproteinase-8 gingival crevicular fluid biomarkers diagnosis |
| title | Active MMP-8 Quantitative Test as an Adjunctive Tool for Early Diagnosis of Periodontitis |
| title_full | Active MMP-8 Quantitative Test as an Adjunctive Tool for Early Diagnosis of Periodontitis |
| title_fullStr | Active MMP-8 Quantitative Test as an Adjunctive Tool for Early Diagnosis of Periodontitis |
| title_full_unstemmed | Active MMP-8 Quantitative Test as an Adjunctive Tool for Early Diagnosis of Periodontitis |
| title_short | Active MMP-8 Quantitative Test as an Adjunctive Tool for Early Diagnosis of Periodontitis |
| title_sort | active mmp 8 quantitative test as an adjunctive tool for early diagnosis of periodontitis |
| topic | periodontitis periodontal disease matrix metalloproteinase-8 gingival crevicular fluid biomarkers diagnosis |
| url | https://www.mdpi.com/2075-4418/11/8/1503 |
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