First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand
Background and Aim: Bovine anaplasmosis (BA) is one of the most important diseases of ruminants worldwide, causing significant economic losses in the livestock industry due to the high morbidity and mortality in susceptible cattle herds. Anaplasma marginale is the main causative agent of BA occurrin...
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| Language: | English |
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Veterinary World
2024-02-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.17/February-2024/18.pdf |
| _version_ | 1797304954312982528 |
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| author | I Putu Gede Yudhi Arjentinia Bamphen Keomoungkhoun Chaiyapas Thamrongyoswittayakul Somboon Sangmaneedet Weerapol Taweenan |
| author_facet | I Putu Gede Yudhi Arjentinia Bamphen Keomoungkhoun Chaiyapas Thamrongyoswittayakul Somboon Sangmaneedet Weerapol Taweenan |
| author_sort | I Putu Gede Yudhi Arjentinia |
| collection | DOAJ |
| description | Background and Aim: Bovine anaplasmosis (BA) is one of the most important diseases of ruminants worldwide, causing significant economic losses in the livestock industry due to the high morbidity and mortality in susceptible cattle herds. Anaplasma marginale is the main causative agent of BA occurring worldwide in tropical and subtropical regions. This study aimed to investigate the first molecular detection and genetic diversity of A. marginale in dairy cattle in Khon Kaen Province, Thailand.
Materials and Methods: Blood samples were collected from 385 lactating cows from 40 dairy farms in five districts of Khon Kaen, regardless of age and health status. To detect A. marginale, all DNA preparations were used for molecular diagnosis using a single polymerase chain reaction with the msp4 gene target. A phylogenetic tree was constructed from the msp4 gene sequences using molecular genetic characterization. Genetic diversity was calculated as haplotype diversity, haplotype number, number of nucleotide differences, nucleotide diversity, and average number of nucleotide differences.
Results: The overall prevalence of A. marginale was 12.72% (49/385). The highest prevalence (17.19%) was found in Ubolratana district, followed by Muang, Kranuan, Khao Suan Kwang, and Nam Phong districts (14.94%, 14.74%, 13.79%, and 3.70%, respectively). Phylogenetic analysis showed that A. marginale was closely related to isolates from Australia (98.96%), China (99.68%), Spain (99.74%), and the USA (99.63%).
Conclusion: The molecular prevalence of BA in dairy cattle is the first to be observed in this area, and the genetic variability with separated clusters shown in the msp4 gene of A. marginale revealed species variation in dairy cattle. This significant genetic diversity contributes to the understanding of the diversity of A. marginale and will be important for the control and prevention of A. marginale in dairy cattle. |
| first_indexed | 2024-03-08T00:18:52Z |
| format | Article |
| id | doaj.art-3d7b9ed80d9941acad7edcbcdc87002a |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-08T00:18:52Z |
| publishDate | 2024-02-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-3d7b9ed80d9941acad7edcbcdc87002a2024-02-16T14:48:17ZengVeterinary WorldVeterinary World0972-89882231-09162024-02-0117238939710.14202/vetworld.2024.389-397First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, ThailandI Putu Gede Yudhi Arjentinia0https://orcid.org/0000-0002-3367-9852Bamphen Keomoungkhoun1https://orcid.org/0000-0001-6221-7019Chaiyapas Thamrongyoswittayakul2https://orcid.org/0000-0001-7514-0428Somboon Sangmaneedet3https://orcid.org/0000-0002-2263-8887Weerapol Taweenan4https://orcid.org/0000-0002-3547-8448Division of Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002 Thailand.Division of Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002 Thailand.Division of Livestock Medicine, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.Division of Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002 Thailand.Division of Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002 Thailand.Background and Aim: Bovine anaplasmosis (BA) is one of the most important diseases of ruminants worldwide, causing significant economic losses in the livestock industry due to the high morbidity and mortality in susceptible cattle herds. Anaplasma marginale is the main causative agent of BA occurring worldwide in tropical and subtropical regions. This study aimed to investigate the first molecular detection and genetic diversity of A. marginale in dairy cattle in Khon Kaen Province, Thailand. Materials and Methods: Blood samples were collected from 385 lactating cows from 40 dairy farms in five districts of Khon Kaen, regardless of age and health status. To detect A. marginale, all DNA preparations were used for molecular diagnosis using a single polymerase chain reaction with the msp4 gene target. A phylogenetic tree was constructed from the msp4 gene sequences using molecular genetic characterization. Genetic diversity was calculated as haplotype diversity, haplotype number, number of nucleotide differences, nucleotide diversity, and average number of nucleotide differences. Results: The overall prevalence of A. marginale was 12.72% (49/385). The highest prevalence (17.19%) was found in Ubolratana district, followed by Muang, Kranuan, Khao Suan Kwang, and Nam Phong districts (14.94%, 14.74%, 13.79%, and 3.70%, respectively). Phylogenetic analysis showed that A. marginale was closely related to isolates from Australia (98.96%), China (99.68%), Spain (99.74%), and the USA (99.63%). Conclusion: The molecular prevalence of BA in dairy cattle is the first to be observed in this area, and the genetic variability with separated clusters shown in the msp4 gene of A. marginale revealed species variation in dairy cattle. This significant genetic diversity contributes to the understanding of the diversity of A. marginale and will be important for the control and prevention of A. marginale in dairy cattle.https://www.veterinaryworld.org/Vol.17/February-2024/18.pdfanaplasma marginalebovine anaplasmosisdairy cattlemolecular prevalence |
| spellingShingle | I Putu Gede Yudhi Arjentinia Bamphen Keomoungkhoun Chaiyapas Thamrongyoswittayakul Somboon Sangmaneedet Weerapol Taweenan First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand Veterinary World anaplasma marginale bovine anaplasmosis dairy cattle molecular prevalence |
| title | First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand |
| title_full | First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand |
| title_fullStr | First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand |
| title_full_unstemmed | First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand |
| title_short | First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand |
| title_sort | first report on the molecular detection and genetic diversity of anaplasma marginale in healthy dairy cattle in khon kaen province thailand |
| topic | anaplasma marginale bovine anaplasmosis dairy cattle molecular prevalence |
| url | https://www.veterinaryworld.org/Vol.17/February-2024/18.pdf |
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