INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS
The production of cellulases, hemicellulases, and starch-degrading enzymes by the thermophilic aerobic fungus Talaromyces emersonii under liquid state culture on various food wastes was investigated. A comprehensive enzyme screening was conducted, which resulted in the identification of spent tea l...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2010-05-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_05_2_0634_Gilleran_HMT_Enzyme_cocktials_Tea_Leaves/546 |
| _version_ | 1818533108932149248 |
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| author | Caroline T. Gilleran Alan T. Hernon Patrick G. Murray Maria G. Tuohy |
| author_facet | Caroline T. Gilleran Alan T. Hernon Patrick G. Murray Maria G. Tuohy |
| author_sort | Caroline T. Gilleran |
| collection | DOAJ |
| description | The production of cellulases, hemicellulases, and starch-degrading enzymes by the thermophilic aerobic fungus Talaromyces emersonii under liquid state culture on various food wastes was investigated. A comprehensive enzyme screening was conducted, which resulted in the identification of spent tea leaves as a potential substrate for hydrolytic enzyme production. The potent, polysaccharide-degrading enzyme-rich cocktail produced when tea leaves were utilised as sole carbon source was analysed at a protein and mRNA level and shown to exhibit high level production of key cellulose and hemicellulose degrading enzymes. As presented in this paper, the crude enzyme preparation produced after 120 h growth of Talaromyces emersonii on used tea leaves is capable of hydrolysing other lignocellulosic materials into their component monosaccharides, generating high value sugar syrups with a host of industrial applications including conversion to fuels and chemicals. |
| first_indexed | 2024-12-11T17:54:20Z |
| format | Article |
| id | doaj.art-3d7c5a629e9644d4af401d8718b8fb75 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-12-11T17:54:20Z |
| publishDate | 2010-05-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-3d7c5a629e9644d4af401d8718b8fb752022-12-22T00:56:07ZengNorth Carolina State UniversityBioResources1930-21262010-05-0152634649INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALSCaroline T. GilleranAlan T. HernonPatrick G. MurrayMaria G. TuohyThe production of cellulases, hemicellulases, and starch-degrading enzymes by the thermophilic aerobic fungus Talaromyces emersonii under liquid state culture on various food wastes was investigated. A comprehensive enzyme screening was conducted, which resulted in the identification of spent tea leaves as a potential substrate for hydrolytic enzyme production. The potent, polysaccharide-degrading enzyme-rich cocktail produced when tea leaves were utilised as sole carbon source was analysed at a protein and mRNA level and shown to exhibit high level production of key cellulose and hemicellulose degrading enzymes. As presented in this paper, the crude enzyme preparation produced after 120 h growth of Talaromyces emersonii on used tea leaves is capable of hydrolysing other lignocellulosic materials into their component monosaccharides, generating high value sugar syrups with a host of industrial applications including conversion to fuels and chemicals.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_05_2_0634_Gilleran_HMT_Enzyme_cocktials_Tea_Leaves/546Lignocellulosic wasteTalaromyces emersoniiSaccharificationCellulaseHemicellulase |
| spellingShingle | Caroline T. Gilleran Alan T. Hernon Patrick G. Murray Maria G. Tuohy INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS BioResources Lignocellulosic waste Talaromyces emersonii Saccharification Cellulase Hemicellulase |
| title | INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS |
| title_full | INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS |
| title_fullStr | INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS |
| title_full_unstemmed | INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS |
| title_short | INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS |
| title_sort | induction of enzyme cocktails by low cost carbon sources for production of monosaccharide rich syrups from plant materials |
| topic | Lignocellulosic waste Talaromyces emersonii Saccharification Cellulase Hemicellulase |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_05_2_0634_Gilleran_HMT_Enzyme_cocktials_Tea_Leaves/546 |
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