DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
Abstract Background DNA barcoding assumes that a biological entity is completely separated from its closest relatives by a barcoding gap, which means that intraspecific genetic distance (from COI sequences) should never be greater than interspecific distances. We investigated the applicability of th...
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| Format: | Article |
| Language: | English |
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BMC
2014-11-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-014-0519-1 |
| _version_ | 1827933540717690880 |
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| author | Silvia Andrade Justi Carolina Dale Cleber Galvão |
| author_facet | Silvia Andrade Justi Carolina Dale Cleber Galvão |
| author_sort | Silvia Andrade Justi |
| collection | DOAJ |
| description | Abstract Background DNA barcoding assumes that a biological entity is completely separated from its closest relatives by a barcoding gap, which means that intraspecific genetic distance (from COI sequences) should never be greater than interspecific distances. We investigated the applicability of this strategy in identifying species of the genus Triatoma from South America. Findings We calculated intra and interspecific Kimura-2-parameter distances between species from the infestans, matogrossensis, sordida and rubrovaria subcomplexes. In every subcomplex examined we observed at least one intraspecific distance greater than interspecific distances. Conclusions Although DNA barcoding is a straightforward approach, it was not applicable for identifying Southern American Triatoma species, which may have diverged recently. Thus, caution should be taken in identifying vector species using this approach, especially in groups where accurate identification of taxa is fundamentally linked to public health issues. |
| first_indexed | 2024-03-13T07:26:13Z |
| format | Article |
| id | doaj.art-3d7cab1654684de597cfe9e6f0641319 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:26:13Z |
| publishDate | 2014-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-3d7cab1654684de597cfe9e6f06413192023-06-04T11:21:34ZengBMCParasites & Vectors1756-33052014-11-01711410.1186/s13071-014-0519-1DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectorsSilvia Andrade Justi0Carolina Dale1Cleber Galvão2Instituto de Biologia, Laboratório de Biologia Evolutiva Teórica e Aplicada, Universidade Federal do Rio de JaneiroLaboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, InstitutoOswaldo Cruz, FIOCRUZLaboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, InstitutoOswaldo Cruz, FIOCRUZAbstract Background DNA barcoding assumes that a biological entity is completely separated from its closest relatives by a barcoding gap, which means that intraspecific genetic distance (from COI sequences) should never be greater than interspecific distances. We investigated the applicability of this strategy in identifying species of the genus Triatoma from South America. Findings We calculated intra and interspecific Kimura-2-parameter distances between species from the infestans, matogrossensis, sordida and rubrovaria subcomplexes. In every subcomplex examined we observed at least one intraspecific distance greater than interspecific distances. Conclusions Although DNA barcoding is a straightforward approach, it was not applicable for identifying Southern American Triatoma species, which may have diverged recently. Thus, caution should be taken in identifying vector species using this approach, especially in groups where accurate identification of taxa is fundamentally linked to public health issues.https://doi.org/10.1186/s13071-014-0519-1TriatominaeChagas diseaseDNA barcodingMolecular identification |
| spellingShingle | Silvia Andrade Justi Carolina Dale Cleber Galvão DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors Parasites & Vectors Triatominae Chagas disease DNA barcoding Molecular identification |
| title | DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors |
| title_full | DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors |
| title_fullStr | DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors |
| title_full_unstemmed | DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors |
| title_short | DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors |
| title_sort | dna barcoding does not separate south american triatoma hemiptera reduviidae chagas disease vectors |
| topic | Triatominae Chagas disease DNA barcoding Molecular identification |
| url | https://doi.org/10.1186/s13071-014-0519-1 |
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