Physiological, Pathological and Pharmacological Interactions of Hydrogen Sulphide and Nitric Oxide in the Myocardium of Rats with Left Ventricular Hypertrophy
Left ventricular hypertrophy (LVH) is characterized by increased myocardium thickness due to increased oxidative stress and downregulation of cystathione γ lyase (CSE) endothelial nitric oxide synthase (eNOS). Upregulation of CSE by hydrogen sulphide (H<sub>2</sub>S) and ENOS by L-argini...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/1/30 |
| Summary: | Left ventricular hypertrophy (LVH) is characterized by increased myocardium thickness due to increased oxidative stress and downregulation of cystathione γ lyase (CSE) endothelial nitric oxide synthase (eNOS). Upregulation of CSE by hydrogen sulphide (H<sub>2</sub>S) and ENOS by L-arginine can arrest the progression of LVH individually. The present study explored the combined treatment of H<sub>2</sub>S and NO in the progression of LVH, and demonstrated that the response is due to H<sub>2</sub>S, NO or formation of either new molecule in physiological, pathological, and pharmacological in vivo settings of LVH. Exogenous administration H<sub>2</sub>S+NO in LVH significantly reduced (all <i>p</i> < 0.05) systolic blood pressure (SBP) and mean arterial pressure (MAP), LV index, heart index and oxidative stress when compared to the LVH group. There was downregulation of CSE mRNA and eNOS in the heart, and exogenous administration of H<sub>2</sub>S+NO groups upregulated eNOS MRNA while CSE MRNA remained downregulated in the hearts of the LVH group. Similar trends were observed with concentrations of H<sub>2</sub>S and NO in the plasma and tissue. It can be concluded that combined treatment of LVH with H<sub>2</sub>S and NO significantly ameliorate the progression of LVH by attenuating systemic hemodynamic and physical indices, and by decreasing oxidative stress. Molecular expression data in the myocardium of LVH depicts that combined treatment upregulated eNOS/NO while it downregulated CSE/H<sub>2</sub>S pathways in in vivo settings, and it is always eNOS/NO pathways which play a major role. |
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| ISSN: | 1467-3037 1467-3045 |