The Moso Bamboo D-Type Cell Cycle Protein Family: Genome Organization, Phylogeny, and Expression Patterns
Cell cycle proteins and cyclin-dependent kinases (CDKs) play a vital role in the control of cell division, and their complexes form a powerful driving force in pushing cell cycle progression. <i>D-type cyclins</i> (<i>CycD</i>s) are essential for interpreting outside mitogeni...
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2024-02-01
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| author | Hui Fang Changhong Mu Jutang Jiang Jian Gao Zhanchao Cheng |
| author_facet | Hui Fang Changhong Mu Jutang Jiang Jian Gao Zhanchao Cheng |
| author_sort | Hui Fang |
| collection | DOAJ |
| description | Cell cycle proteins and cyclin-dependent kinases (CDKs) play a vital role in the control of cell division, and their complexes form a powerful driving force in pushing cell cycle progression. <i>D-type cyclins</i> (<i>CycD</i>s) are essential for interpreting outside mitogenic signals and regulating the G1 phase. At least 19 distinct <i>CycD</i>s are present in the Moso bamboo (<i>Phyllostachys edulis</i>) genome, belonging to subgroups identified previously in other plants. Silico analysis validated the representative distinctive cyclin domains of each <i>CycD</i> in Moso bamboo, revealing that the genomic architectures of these genes were identical to those of their orthologs in <i>Arabidopsis</i> and rice. Both the phylogeny and covariance suggested that <i>PheCycD</i>s were structurally conserved and had undergone gene duplication. Transcriptome data analysis related to different tissues revealed that most <i>CycD</i>s were highly expressed in Moso bamboo shoots. The addition of growth hormone (NAA) significantly increased the transcript levels of <i>PheCycD4;4</i>, <i>D5;1</i>, <i>D5;2</i>, and <i>D6;1</i> for a short period of time (6 h), and inhibitors (PCIB) also greatly decreased their expression. These results improved the understanding of <i>PheCycD</i>s in our study, notably in relation to auxin response, and offered an initial insight into the expression pattern and functional mining of the <i>PheCycD</i> gene family. |
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| language | English |
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| spelling | doaj.art-3d83b91cfcdd44b6a9c29ec8257daff22024-02-23T15:16:51ZengMDPI AGForests1999-49072024-02-0115228910.3390/f15020289The Moso Bamboo D-Type Cell Cycle Protein Family: Genome Organization, Phylogeny, and Expression PatternsHui Fang0Changhong Mu1Jutang Jiang2Jian Gao3Zhanchao Cheng4Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, ChinaCell cycle proteins and cyclin-dependent kinases (CDKs) play a vital role in the control of cell division, and their complexes form a powerful driving force in pushing cell cycle progression. <i>D-type cyclins</i> (<i>CycD</i>s) are essential for interpreting outside mitogenic signals and regulating the G1 phase. At least 19 distinct <i>CycD</i>s are present in the Moso bamboo (<i>Phyllostachys edulis</i>) genome, belonging to subgroups identified previously in other plants. Silico analysis validated the representative distinctive cyclin domains of each <i>CycD</i> in Moso bamboo, revealing that the genomic architectures of these genes were identical to those of their orthologs in <i>Arabidopsis</i> and rice. Both the phylogeny and covariance suggested that <i>PheCycD</i>s were structurally conserved and had undergone gene duplication. Transcriptome data analysis related to different tissues revealed that most <i>CycD</i>s were highly expressed in Moso bamboo shoots. The addition of growth hormone (NAA) significantly increased the transcript levels of <i>PheCycD4;4</i>, <i>D5;1</i>, <i>D5;2</i>, and <i>D6;1</i> for a short period of time (6 h), and inhibitors (PCIB) also greatly decreased their expression. These results improved the understanding of <i>PheCycD</i>s in our study, notably in relation to auxin response, and offered an initial insight into the expression pattern and functional mining of the <i>PheCycD</i> gene family.https://www.mdpi.com/1999-4907/15/2/289Moso bambooD-type cyclinsphylogenetic analysisgene duplicationgene expressionauxin |
| spellingShingle | Hui Fang Changhong Mu Jutang Jiang Jian Gao Zhanchao Cheng The Moso Bamboo D-Type Cell Cycle Protein Family: Genome Organization, Phylogeny, and Expression Patterns Forests Moso bamboo D-type cyclins phylogenetic analysis gene duplication gene expression auxin |
| title | The Moso Bamboo D-Type Cell Cycle Protein Family: Genome Organization, Phylogeny, and Expression Patterns |
| title_full | The Moso Bamboo D-Type Cell Cycle Protein Family: Genome Organization, Phylogeny, and Expression Patterns |
| title_fullStr | The Moso Bamboo D-Type Cell Cycle Protein Family: Genome Organization, Phylogeny, and Expression Patterns |
| title_full_unstemmed | The Moso Bamboo D-Type Cell Cycle Protein Family: Genome Organization, Phylogeny, and Expression Patterns |
| title_short | The Moso Bamboo D-Type Cell Cycle Protein Family: Genome Organization, Phylogeny, and Expression Patterns |
| title_sort | moso bamboo d type cell cycle protein family genome organization phylogeny and expression patterns |
| topic | Moso bamboo D-type cyclins phylogenetic analysis gene duplication gene expression auxin |
| url | https://www.mdpi.com/1999-4907/15/2/289 |
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