Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish
Abstract Enterococcus faecalis is associated with streptococcosis like infection in fish. A whole-genome sequence study was conducted to investigate the virulence factor and antibiotic-resistance genes in three fish pathogenic E. faecalis. Genomic DNA was extracted from three strains of E. faecalis...
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| Format: | Article |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-25968-8 |
| _version_ | 1811175931102887936 |
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| author | Tasmina Akter Md. Najmul Haque Rakib Ehsan Sulav Indra Paul Md. Javed Foysal Alfred Chin Yen Tay Md. Tofazzal Islam Md. Mahbubur Rahman |
| author_facet | Tasmina Akter Md. Najmul Haque Rakib Ehsan Sulav Indra Paul Md. Javed Foysal Alfred Chin Yen Tay Md. Tofazzal Islam Md. Mahbubur Rahman |
| author_sort | Tasmina Akter |
| collection | DOAJ |
| description | Abstract Enterococcus faecalis is associated with streptococcosis like infection in fish. A whole-genome sequence study was conducted to investigate the virulence factor and antibiotic-resistance genes in three fish pathogenic E. faecalis. Genomic DNA was extracted from three strains of E. faecalis isolated from streptococcosis infected Nile tilapia (strains BF1B1 and BFFF11) and Thai sarpunti (strain BFPS6). The whole genome sequences of these three strains were performed using a MiSeq sequencer (Illumina, Inc.). All three strains conserved 69 virulence factor such as genes associated with protection against oxidative stress, bacterial cell wall synthesis, gelatinase toxin, multiple biofilm-associated genes and capsule producing genes. Moreover, 39 antibiotic-resistance genes against sixteen major groups of antibiotics were identified in the genome sequences of all three strains. The most commonly used antibiotic Tetracycline resistance genes were found only in BFPS6 strain, whereas, Bacteriocin synthesis genes were identified in both BFFF11 and BFPS6 strain. Phylogenetic analysis revealed that strains BF1B1 and BFFF1 form a different cluster than BFPS6. This is one of the first whole-genome sequence study of fish pathogenic E. faecalis, unfold new information on the virulence factor and Antibiotic resistance genes linked to pathogenicity in fish. |
| first_indexed | 2024-04-10T19:43:57Z |
| format | Article |
| id | doaj.art-3d8795a7add94c0f920df6b8b4440253 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T19:43:57Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3d8795a7add94c0f920df6b8b44402532023-01-29T12:08:26ZengNature PortfolioScientific Reports2045-23222023-01-0113111210.1038/s41598-022-25968-8Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fishTasmina Akter0Md. Najmul Haque1Rakib Ehsan2Sulav Indra Paul3Md. Javed Foysal4Alfred Chin Yen Tay5Md. Tofazzal Islam6Md. Mahbubur Rahman7Institute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityDepartment of Animal Science and Veterinary Medicine, Bangabandhu Sheikh Mujibur Rahman Science and Technology UniversityInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural UniversitySchool of Molecular and Life Sciences, Curtin UniversityMarshall Centre for Infectious Disease Research and Training. School of Biomedical Sciences, University of Western AustraliaInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityAbstract Enterococcus faecalis is associated with streptococcosis like infection in fish. A whole-genome sequence study was conducted to investigate the virulence factor and antibiotic-resistance genes in three fish pathogenic E. faecalis. Genomic DNA was extracted from three strains of E. faecalis isolated from streptococcosis infected Nile tilapia (strains BF1B1 and BFFF11) and Thai sarpunti (strain BFPS6). The whole genome sequences of these three strains were performed using a MiSeq sequencer (Illumina, Inc.). All three strains conserved 69 virulence factor such as genes associated with protection against oxidative stress, bacterial cell wall synthesis, gelatinase toxin, multiple biofilm-associated genes and capsule producing genes. Moreover, 39 antibiotic-resistance genes against sixteen major groups of antibiotics were identified in the genome sequences of all three strains. The most commonly used antibiotic Tetracycline resistance genes were found only in BFPS6 strain, whereas, Bacteriocin synthesis genes were identified in both BFFF11 and BFPS6 strain. Phylogenetic analysis revealed that strains BF1B1 and BFFF1 form a different cluster than BFPS6. This is one of the first whole-genome sequence study of fish pathogenic E. faecalis, unfold new information on the virulence factor and Antibiotic resistance genes linked to pathogenicity in fish.https://doi.org/10.1038/s41598-022-25968-8 |
| spellingShingle | Tasmina Akter Md. Najmul Haque Rakib Ehsan Sulav Indra Paul Md. Javed Foysal Alfred Chin Yen Tay Md. Tofazzal Islam Md. Mahbubur Rahman Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish Scientific Reports |
| title | Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish |
| title_full | Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish |
| title_fullStr | Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish |
| title_full_unstemmed | Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish |
| title_short | Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish |
| title_sort | virulence and antibiotic resistance genes in enterococcus faecalis associated with streptococcosis disease in fish |
| url | https://doi.org/10.1038/s41598-022-25968-8 |
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