Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures
Conventional bio-based succinic acid production involves anaerobic bacterial fermentation of pure sugars. This study explored a new route for directly producing succinic acid from minimally-pretreated lignocellulosic biomass via a consolidated bioprocessing technology employing a mixed lignocellulol...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-06-01
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| Series: | Fermentation |
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| Online Access: | http://www.mdpi.com/2311-5637/3/3/30 |
| _version_ | 1818149871923757056 |
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| author | Jerico Alcantara Andro Mondala Logan Hughey Shaun Shields |
| author_facet | Jerico Alcantara Andro Mondala Logan Hughey Shaun Shields |
| author_sort | Jerico Alcantara |
| collection | DOAJ |
| description | Conventional bio-based succinic acid production involves anaerobic bacterial fermentation of pure sugars. This study explored a new route for directly producing succinic acid from minimally-pretreated lignocellulosic biomass via a consolidated bioprocessing technology employing a mixed lignocellulolytic and acidogenic fungal co-culture. The process involved a solid-state pre-fermentation stage followed by a two-phase slurry fermentation stage. During the solid-state pre-fermentation stage, Aspergillus niger and Trichoderma reesei were co-cultured in a nitrogen-rich substrate (e.g., soybean hull) to induce cellulolytic enzyme activity. The ligninolytic fungus Phanerochaete chrysosporium was grown separately on carbon-rich birch wood chips to induce ligninolytic enzymes, rendering the biomass more susceptible to cellulase attack. The solid-state pre-cultures were then combined in a slurry fermentation culture to achieve simultaneous enzymatic cellulolysis and succinic acid production. This approach generated succinic acid at maximum titers of 32.43 g/L after 72 h of batch slurry fermentation (~10 g/L production), and 61.12 g/L after 36 h of addition of fresh birch wood chips at the onset of the slurry fermentation stage (~26 g/L production). Based on this result, this approach is a promising alternative to current bacterial succinic acid production due to its minimal substrate pretreatment requirements, which could reduce production costs. |
| first_indexed | 2024-12-11T13:13:56Z |
| format | Article |
| id | doaj.art-3d8dd34bfa304d359769de29ef30960f |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-12-11T13:13:56Z |
| publishDate | 2017-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-3d8dd34bfa304d359769de29ef30960f2022-12-22T01:06:06ZengMDPI AGFermentation2311-56372017-06-01333010.3390/fermentation3030030fermentation3030030Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal CulturesJerico Alcantara0Andro Mondala1Logan Hughey2Shaun Shields3Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, MI 49008, USADepartment of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, MI 49008, USADepartment of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, MI 49008, USADepartment of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, MI 49008, USAConventional bio-based succinic acid production involves anaerobic bacterial fermentation of pure sugars. This study explored a new route for directly producing succinic acid from minimally-pretreated lignocellulosic biomass via a consolidated bioprocessing technology employing a mixed lignocellulolytic and acidogenic fungal co-culture. The process involved a solid-state pre-fermentation stage followed by a two-phase slurry fermentation stage. During the solid-state pre-fermentation stage, Aspergillus niger and Trichoderma reesei were co-cultured in a nitrogen-rich substrate (e.g., soybean hull) to induce cellulolytic enzyme activity. The ligninolytic fungus Phanerochaete chrysosporium was grown separately on carbon-rich birch wood chips to induce ligninolytic enzymes, rendering the biomass more susceptible to cellulase attack. The solid-state pre-cultures were then combined in a slurry fermentation culture to achieve simultaneous enzymatic cellulolysis and succinic acid production. This approach generated succinic acid at maximum titers of 32.43 g/L after 72 h of batch slurry fermentation (~10 g/L production), and 61.12 g/L after 36 h of addition of fresh birch wood chips at the onset of the slurry fermentation stage (~26 g/L production). Based on this result, this approach is a promising alternative to current bacterial succinic acid production due to its minimal substrate pretreatment requirements, which could reduce production costs.http://www.mdpi.com/2311-5637/3/3/30consolidated bioprocessingfungisolid-phase fermentationbio-based chemicalslignocellulose biomassmixed cultures |
| spellingShingle | Jerico Alcantara Andro Mondala Logan Hughey Shaun Shields Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures Fermentation consolidated bioprocessing fungi solid-phase fermentation bio-based chemicals lignocellulose biomass mixed cultures |
| title | Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures |
| title_full | Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures |
| title_fullStr | Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures |
| title_full_unstemmed | Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures |
| title_short | Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures |
| title_sort | direct succinic acid production from minimally pretreated biomass using sequential solid state and slurry fermentation with mixed fungal cultures |
| topic | consolidated bioprocessing fungi solid-phase fermentation bio-based chemicals lignocellulose biomass mixed cultures |
| url | http://www.mdpi.com/2311-5637/3/3/30 |
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