Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stress
Heat and drought are among the leading environmental stresses which have a major impact on plant development. In our research, identification and characterization of differentially expressed genes (DEGs) regulating the response of wheat to drought, heat and combined stress was carried out. We analyz...
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| Format: | Article |
| Language: | English |
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International Library of Sciences
2021-01-01
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| Series: | Highlights in BioScience |
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| Online Access: | http://highlightsin.org/index.php/bioscience/article/view/46 |
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| author | Alsamman M. Alsamman Ratiba Bousba Michael Baum Aladdin Hamwieh Nourhan Fouad |
| author_facet | Alsamman M. Alsamman Ratiba Bousba Michael Baum Aladdin Hamwieh Nourhan Fouad |
| author_sort | Alsamman M. Alsamman |
| collection | DOAJ |
| description | Heat and drought are among the leading environmental stresses which have a major impact on plant development. In our research, identification and characterization of differentially expressed genes (DEGs) regulating the response of wheat to drought, heat and combined stress was carried out. We analyzed data from the Gene Expression Omnibus database (GEO) microarrays containing 24 samples of wheat, which were categorized by different treatments (control: ctrl, drought: D, heat: H, and mixed: HD). Significant DEGs were examined for gene annotation, gene ontology, co-expression, protein-protein interaction (PPI) and their heterogeneity and consistency through drought, heat and combined stress was also studied. Genes such as gyrB, C6orf132 homolog, PYR1 were highly associated with wheat response to drought with P-value (-log10) of 9.3, 7.3, 6.4, and logFC of -3.9, 2.0, 1.6, respectively. DEGs associated with drought tolerance were highly related to the protein domains of lipid-transfer (LTP). Wheat response to heat stress was strongly associated with genes such as RuBisCO activase B, small heat shock, LTP3, YLS3, At2g33490, PETH with p-values (-log10) ranging from 9.3 to 12.3. In addition, a relatively high number of protein interactions involved the SDH, PEPCK, and G6PD genes under heat stress. |
| first_indexed | 2024-04-12T18:16:42Z |
| format | Article |
| id | doaj.art-3d916da7046943469ee1b14642d94ffe |
| institution | Directory Open Access Journal |
| issn | 2682-4043 |
| language | English |
| last_indexed | 2024-04-12T18:16:42Z |
| publishDate | 2021-01-01 |
| publisher | International Library of Sciences |
| record_format | Article |
| series | Highlights in BioScience |
| spelling | doaj.art-3d916da7046943469ee1b14642d94ffe2022-12-22T03:21:34ZengInternational Library of SciencesHighlights in BioScience2682-40432021-01-014010.36462/H.BioSci.20210443Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stressAlsamman M. Alsamman0Ratiba Bousba1Michael Baum2Aladdin Hamwieh3Nourhan Fouad4MGGM laboratory, Agricultural Genetic Engineering Research Institute, Agricultural Research CenterGenetics, Biochemistry, and Plant Biotechnology Laboratory, Department of Biology and Plant Ecology, Mentouri Brothers UniversityInternational Center for Agricultural Research in the Dry Areas (ICARDA), PO Box 6299, RabatInternational Center for Agricultural Research in the Dry Areas (ICARDA), PO Box 2416, CairoInternational Center for Agricultural Research in the Dry Areas (ICARDA), PO Box 2416, CairoHeat and drought are among the leading environmental stresses which have a major impact on plant development. In our research, identification and characterization of differentially expressed genes (DEGs) regulating the response of wheat to drought, heat and combined stress was carried out. We analyzed data from the Gene Expression Omnibus database (GEO) microarrays containing 24 samples of wheat, which were categorized by different treatments (control: ctrl, drought: D, heat: H, and mixed: HD). Significant DEGs were examined for gene annotation, gene ontology, co-expression, protein-protein interaction (PPI) and their heterogeneity and consistency through drought, heat and combined stress was also studied. Genes such as gyrB, C6orf132 homolog, PYR1 were highly associated with wheat response to drought with P-value (-log10) of 9.3, 7.3, 6.4, and logFC of -3.9, 2.0, 1.6, respectively. DEGs associated with drought tolerance were highly related to the protein domains of lipid-transfer (LTP). Wheat response to heat stress was strongly associated with genes such as RuBisCO activase B, small heat shock, LTP3, YLS3, At2g33490, PETH with p-values (-log10) ranging from 9.3 to 12.3. In addition, a relatively high number of protein interactions involved the SDH, PEPCK, and G6PD genes under heat stress.http://highlightsin.org/index.php/bioscience/article/view/46wheat, drought, heat, combined stress, gene expression |
| spellingShingle | Alsamman M. Alsamman Ratiba Bousba Michael Baum Aladdin Hamwieh Nourhan Fouad Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stress Highlights in BioScience wheat, drought, heat, combined stress, gene expression |
| title | Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stress |
| title_full | Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stress |
| title_fullStr | Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stress |
| title_full_unstemmed | Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stress |
| title_short | Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stress |
| title_sort | comprehensive analysis of the gene expression profile of wheat at the crossroads of heat drought and combined stress |
| topic | wheat, drought, heat, combined stress, gene expression |
| url | http://highlightsin.org/index.php/bioscience/article/view/46 |
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