Compromised dental cells viability following teeth-whitening exposure
Abstract This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay model. 30 teeth were exposed to 5% or 16% CP gel (4 h daily) for 2-weeks. The enamel organic content was measured with the...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-94745-w |
| _version_ | 1818419902780801024 |
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| author | Ola Redha Morteza Mazinanian Sabrina Nguyen Dong Ok Son Monika Lodyga Boris Hinz Marianne Odlyha Ailbhe McDonald Laurent Bozec |
| author_facet | Ola Redha Morteza Mazinanian Sabrina Nguyen Dong Ok Son Monika Lodyga Boris Hinz Marianne Odlyha Ailbhe McDonald Laurent Bozec |
| author_sort | Ola Redha |
| collection | DOAJ |
| description | Abstract This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay model. 30 teeth were exposed to 5% or 16% CP gel (4 h daily) for 2-weeks. The enamel organic content was measured with thermogravimetry. The time-dependent viability of human dental pulp stem cells (HDPSCs) and gingival fibroblast cells (HGFCs) following either indirect exposure to 3 commercially available concentrations of CP gel using an in-vitro dentin perfusion assay or direct exposure to 5% H2O2 were investigated by evaluating change in cell morphology and by hemocytometry. The 5% and 16% CP produced a significantly lower (p < 0.001) enamel protein content (by weight) when compared to the control. The organic content in enamel varied accordingly to the CP treatment: for the 16% and 5% CP treatment groups, a variation of 4.0% and 5.4%, respectively, was observed with no significant difference. The cell viability of HDPSCs decreased exponentially over time for all groups. Within the limitation of this in-vitro study, we conclude that even low concentrations of H2O2 and CP result in a deleterious change in enamel protein content and compromise the viability of HGFCs and HDPSCs. These effects should be observed in-vivo. |
| first_indexed | 2024-12-14T12:45:57Z |
| format | Article |
| id | doaj.art-3d9876734b234b128f12ac9ecadc35ed |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T12:45:57Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3d9876734b234b128f12ac9ecadc35ed2022-12-21T23:00:46ZengNature PortfolioScientific Reports2045-23222021-07-011111910.1038/s41598-021-94745-wCompromised dental cells viability following teeth-whitening exposureOla Redha0Morteza Mazinanian1Sabrina Nguyen2Dong Ok Son3Monika Lodyga4Boris Hinz5Marianne Odlyha6Ailbhe McDonald7Laurent Bozec8Division of Prosthodontics, UCL Eastman Dental Institute, University College LondonDivision of Prosthodontics, UCL Eastman Dental Institute, University College LondonFaculty of Dentistry, University of TorontoFaculty of Dentistry, University of TorontoFaculty of Dentistry, University of TorontoFaculty of Dentistry, University of TorontoDepartment of Biological Sciences, Birkbeck, University of LondonDivision of Prosthodontics, UCL Eastman Dental Institute, University College LondonDivision of Biomaterials and Tissue Engineering, UCL Eastman Dental InstituteAbstract This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay model. 30 teeth were exposed to 5% or 16% CP gel (4 h daily) for 2-weeks. The enamel organic content was measured with thermogravimetry. The time-dependent viability of human dental pulp stem cells (HDPSCs) and gingival fibroblast cells (HGFCs) following either indirect exposure to 3 commercially available concentrations of CP gel using an in-vitro dentin perfusion assay or direct exposure to 5% H2O2 were investigated by evaluating change in cell morphology and by hemocytometry. The 5% and 16% CP produced a significantly lower (p < 0.001) enamel protein content (by weight) when compared to the control. The organic content in enamel varied accordingly to the CP treatment: for the 16% and 5% CP treatment groups, a variation of 4.0% and 5.4%, respectively, was observed with no significant difference. The cell viability of HDPSCs decreased exponentially over time for all groups. Within the limitation of this in-vitro study, we conclude that even low concentrations of H2O2 and CP result in a deleterious change in enamel protein content and compromise the viability of HGFCs and HDPSCs. These effects should be observed in-vivo.https://doi.org/10.1038/s41598-021-94745-w |
| spellingShingle | Ola Redha Morteza Mazinanian Sabrina Nguyen Dong Ok Son Monika Lodyga Boris Hinz Marianne Odlyha Ailbhe McDonald Laurent Bozec Compromised dental cells viability following teeth-whitening exposure Scientific Reports |
| title | Compromised dental cells viability following teeth-whitening exposure |
| title_full | Compromised dental cells viability following teeth-whitening exposure |
| title_fullStr | Compromised dental cells viability following teeth-whitening exposure |
| title_full_unstemmed | Compromised dental cells viability following teeth-whitening exposure |
| title_short | Compromised dental cells viability following teeth-whitening exposure |
| title_sort | compromised dental cells viability following teeth whitening exposure |
| url | https://doi.org/10.1038/s41598-021-94745-w |
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