Characterisation of Anaerobes Isolated from Various Clinical Samples: A Cross-sectional Study
Introduction: Anaerobes are an important cause of infections but are often neglected. These infections can range from simple abscesses to life-threatening infections. The isolation of anaerobes is crucial for administering appropriate antibiotic therapy. Aim: To investigate the profile of anaer...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2023-10-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://www.jcdr.net/articles/PDF/18608/65429_CE[Ra1]_F(IS)_QC(SHEE_IS)_PF1(AG_SL)_PFA(OM)_PB(AG_KM)_PN(KM).pdf |
Summary: | Introduction: Anaerobes are an important cause of infections
but are often neglected. These infections can range from
simple abscesses to life-threatening infections. The isolation
of anaerobes is crucial for administering appropriate antibiotic
therapy.
Aim: To investigate the profile of anaerobes in various clinical
samples, including deep-seated skin and tissue infections,
aspirated body fluids, and tissue biopsies.
Materials and Methods: A cross-sectional study was conducted
on a total of 100 samples at the Department of Microbiology,
ABVIMS and Dr RML Hospital, New Delhi, from November 2019
to March 2021. Aspirations from deep-seated abscesses, body
fluids, intraoperative samples, and tissue biopsies meeting
the criteria for anaerobic culture were included. Simultaneous
processing for the detection of aerobes was also performed.
Anoxomat III anaerobic culture system was used to create an
anaerobic environment. Robertson Cooked Meat (RCM) broth
was used, and subculture was conducted on 10% Blood Agar
(BA). Presumptive identification was performed using gram stain,
catalase test (15% hydrogen peroxide), metronidazole disc (5 µg),
special potency disc (vancomycin 5 µg, kanamycin 1000 µg,
colistin 10 µg), and aerotolerance test. The Vitek 2 compact
ID system was used for the final identification of anaerobes.
Statistical Package for Social Sciences (SPSS) software, IBM
manufacturer, Chicago, USA, ver 21.0, was utilised.
Results: The isolation rate of anaerobes was 17 (17%), with
Bacteroides fragilis being the predominant organism (6; 35.29%),
followed by Actinomyces (2; 11.76%), Clostridium (2; 11.76%),
Peptostreptococcus (2; 11.76%), and Prevotella species (2;
11.76%). Isolation was observed from diverse anatomic sites,
with pus aspirates constituting the majority of the isolates
(9; 52.94%), followed by brain abscesses (3; 17.65%), liver
abscesses, peritoneal fluid (2; 11.76%), and tonsillar abscess
(1; 5.89%). Five (29.41%) infections were polymicrobial, while
12 (70.59%) were monomicrobial in nature.
Conclusion: Anaerobes are emerging as an important causative
agents in a variety of diverse and heterogeneous pyogenic
infections. This study demonstrates their isolation from various
infection sites. Therefore, routine anaerobic cultures should be
conducted alongside aerobic cultures, and the importance of
anaerobes in clinical infections should not be underestimated. |
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ISSN: | 2249-782X 0973-709X |