Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations
We aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated <i>Certastes cerastes</i> venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3]...
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MDPI AG
2021-11-01
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| 丛编: | Molecules |
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| 在线阅读: | https://www.mdpi.com/1420-3049/26/22/7057 |
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| author | Mostafa I. Abdelglil Sanaa O. Abdallah Mohamed A. El-Desouky Mohammad Y. Alfaifi Serag Eldin I. Elbehairi Aly F. Mohamed |
| author_facet | Mostafa I. Abdelglil Sanaa O. Abdallah Mohamed A. El-Desouky Mohammad Y. Alfaifi Serag Eldin I. Elbehairi Aly F. Mohamed |
| author_sort | Mostafa I. Abdelglil |
| collection | DOAJ |
| description | We aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated <i>Certastes cerastes</i> venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3] cancer cells. Toxicity to [A549] and [PC3] cells was concentration and cell type dependent. In comparison to controls, apoptotic genes showed a significant upregulation of P53 and Casp-3 and a downregulation of Bcl-2. Also, induced elevated DNA accumulation in the [S] phase post PC3 cell treatment with IRRV and CV, as well as a significant DNA accumulation at G2/M phase after IRRV treatment of A549 cells. In contrast, PC3 cells showed a negligible cellular DNA accumulation after PEE treatment. Glutathione reductase [GR] was reduced in case of PC3 and A549 cell treated with IRRV, CV, and PEE compared with its values in untreated cell control. The Malondialdehyde [MDA] values in both cells recorded a significant elevation post IRRV treatment compared to the rest of the treatment regimen and untreated cell control. Similarly, IRRV and CV-PEE mix showed obviously higher reactive oxygen species [ROS] values than PC3 and A549 cell treatments with CV and PEE. |
| first_indexed | 2024-03-10T05:13:33Z |
| format | Article |
| id | doaj.art-3d9e2803716c44b9ab31423c2a05cc2a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:13:33Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3d9e2803716c44b9ab31423c2a05cc2a2023-11-23T00:37:58ZengMDPI AGMolecules1420-30492021-11-012622705710.3390/molecules26227057Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological AlterationsMostafa I. Abdelglil0Sanaa O. Abdallah1Mohamed A. El-Desouky2Mohammad Y. Alfaifi3Serag Eldin I. Elbehairi4Aly F. Mohamed5Faculty of Sciences, Cairo University, Giza 12613, EgyptFaculty of Sciences, Cairo University, Giza 12613, EgyptFaculty of Sciences, Cairo University, Giza 12613, EgyptBiology Department, Faculty of Science, King Khalid University, Abha 9004, Saudi ArabiaBiology Department, Faculty of Science, King Khalid University, Abha 9004, Saudi ArabiaThe International Center for Training & Advanced Researches (ICTAR–Egypt), Cairo 11647, EgyptWe aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated <i>Certastes cerastes</i> venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3] cancer cells. Toxicity to [A549] and [PC3] cells was concentration and cell type dependent. In comparison to controls, apoptotic genes showed a significant upregulation of P53 and Casp-3 and a downregulation of Bcl-2. Also, induced elevated DNA accumulation in the [S] phase post PC3 cell treatment with IRRV and CV, as well as a significant DNA accumulation at G2/M phase after IRRV treatment of A549 cells. In contrast, PC3 cells showed a negligible cellular DNA accumulation after PEE treatment. Glutathione reductase [GR] was reduced in case of PC3 and A549 cell treated with IRRV, CV, and PEE compared with its values in untreated cell control. The Malondialdehyde [MDA] values in both cells recorded a significant elevation post IRRV treatment compared to the rest of the treatment regimen and untreated cell control. Similarly, IRRV and CV-PEE mix showed obviously higher reactive oxygen species [ROS] values than PC3 and A549 cell treatments with CV and PEE.https://www.mdpi.com/1420-3049/26/22/7057PEEIRR VA549, PC3apoptoticIC<sub>50</sub>CV |
| spellingShingle | Mostafa I. Abdelglil Sanaa O. Abdallah Mohamed A. El-Desouky Mohammad Y. Alfaifi Serag Eldin I. Elbehairi Aly F. Mohamed Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations Molecules PEE IRR V A549, PC3 apoptotic IC<sub>50</sub> CV |
| title | Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations |
| title_full | Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations |
| title_fullStr | Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations |
| title_full_unstemmed | Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations |
| title_short | Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations |
| title_sort | evaluation of the anticancer potential of crude irradiated i cerastes cerastes i snake venom and propolis ethanolic extract related biological alterations |
| topic | PEE IRR V A549, PC3 apoptotic IC<sub>50</sub> CV |
| url | https://www.mdpi.com/1420-3049/26/22/7057 |
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