Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP
Abstract A single injection of platelet-rich plasma (PRP) or stromal vascular fraction (SVF) in treating neurological ailments suggests promise; however, there is limited evidence of the efficacy of combination therapy. This trial aimed to determine whether combining SVF and PRP could provide furthe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-02-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52962-z |
| _version_ | 1797274799533195264 |
|---|---|
| author | Mariam F. Farid Noha A. E. Yasin Asmaa K. Al-Mokaddem Marwa A. Ibrahim Yara S. Abouelela Hamdy rizk |
| author_facet | Mariam F. Farid Noha A. E. Yasin Asmaa K. Al-Mokaddem Marwa A. Ibrahim Yara S. Abouelela Hamdy rizk |
| author_sort | Mariam F. Farid |
| collection | DOAJ |
| description | Abstract A single injection of platelet-rich plasma (PRP) or stromal vascular fraction (SVF) in treating neurological ailments suggests promise; however, there is limited evidence of the efficacy of combination therapy. This trial aimed to determine whether combining SVF and PRP could provide further therapeutic effects in treating multiple sclerosis (MS). Fifteen Persian cats were separated into three groups (n = 5): group I (control negative), and group II (control positive); EB was injected intrathecally into the spinal cord and then treated 14 days later with intrathecal phosphate buffered saline injection, and group III (SVF + PRP), cats were injected intrathecally with EB through the spinal cord, followed by a combination of SVF and PRP 14 days after induction. Therapeutic effects were evaluated using the Basso–Beattie–Bresnahan scale throughout the treatment timeline and at the end. Together with morphological, MRI scan, immunohistochemical, transmission electron microscopy, and gene expression investigations. The results demonstrated that combining SVF and PRP successfully reduced lesion intensity on gross inspection and MRI. In addition to increased immunoreactivity to Olig2 and MBP and decreased immunoreactivity to Bax and GFAP, there was a significant improvement in BBB scores and an increase in neurotrophic factor (BDNF, NGF, and SDF) expression when compared to the positive control group. Finally, intrathecal SVF + PRP is the most promising and safe therapy for multiple sclerosis, resulting in clinical advantages such as functional recovery, MRI enhancement, and axonal remyelination. |
| first_indexed | 2024-03-07T15:03:10Z |
| format | Article |
| id | doaj.art-3d9efd907eef46e7987910468fd0ea9a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:03:10Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3d9efd907eef46e7987910468fd0ea9a2024-03-05T19:00:11ZengNature PortfolioScientific Reports2045-23222024-02-0114111510.1038/s41598-024-52962-zCombined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAPMariam F. Farid0Noha A. E. Yasin1Asmaa K. Al-Mokaddem2Marwa A. Ibrahim3Yara S. Abouelela4Hamdy rizk5Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Pathology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo UniversityAbstract A single injection of platelet-rich plasma (PRP) or stromal vascular fraction (SVF) in treating neurological ailments suggests promise; however, there is limited evidence of the efficacy of combination therapy. This trial aimed to determine whether combining SVF and PRP could provide further therapeutic effects in treating multiple sclerosis (MS). Fifteen Persian cats were separated into three groups (n = 5): group I (control negative), and group II (control positive); EB was injected intrathecally into the spinal cord and then treated 14 days later with intrathecal phosphate buffered saline injection, and group III (SVF + PRP), cats were injected intrathecally with EB through the spinal cord, followed by a combination of SVF and PRP 14 days after induction. Therapeutic effects were evaluated using the Basso–Beattie–Bresnahan scale throughout the treatment timeline and at the end. Together with morphological, MRI scan, immunohistochemical, transmission electron microscopy, and gene expression investigations. The results demonstrated that combining SVF and PRP successfully reduced lesion intensity on gross inspection and MRI. In addition to increased immunoreactivity to Olig2 and MBP and decreased immunoreactivity to Bax and GFAP, there was a significant improvement in BBB scores and an increase in neurotrophic factor (BDNF, NGF, and SDF) expression when compared to the positive control group. Finally, intrathecal SVF + PRP is the most promising and safe therapy for multiple sclerosis, resulting in clinical advantages such as functional recovery, MRI enhancement, and axonal remyelination.https://doi.org/10.1038/s41598-024-52962-z |
| spellingShingle | Mariam F. Farid Noha A. E. Yasin Asmaa K. Al-Mokaddem Marwa A. Ibrahim Yara S. Abouelela Hamdy rizk Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP Scientific Reports |
| title | Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP |
| title_full | Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP |
| title_fullStr | Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP |
| title_full_unstemmed | Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP |
| title_short | Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP |
| title_sort | combined laser activated svf and prp remodeled spinal sclerosis via activation of olig 2 mbp and neurotrophic factors and inhibition of bax and gfap |
| url | https://doi.org/10.1038/s41598-024-52962-z |
| work_keys_str_mv | AT mariamffarid combinedlaseractivatedsvfandprpremodeledspinalsclerosisviaactivationofolig2mbpandneurotrophicfactorsandinhibitionofbaxandgfap AT nohaaeyasin combinedlaseractivatedsvfandprpremodeledspinalsclerosisviaactivationofolig2mbpandneurotrophicfactorsandinhibitionofbaxandgfap AT asmaakalmokaddem combinedlaseractivatedsvfandprpremodeledspinalsclerosisviaactivationofolig2mbpandneurotrophicfactorsandinhibitionofbaxandgfap AT marwaaibrahim combinedlaseractivatedsvfandprpremodeledspinalsclerosisviaactivationofolig2mbpandneurotrophicfactorsandinhibitionofbaxandgfap AT yarasabouelela combinedlaseractivatedsvfandprpremodeledspinalsclerosisviaactivationofolig2mbpandneurotrophicfactorsandinhibitionofbaxandgfap AT hamdyrizk combinedlaseractivatedsvfandprpremodeledspinalsclerosisviaactivationofolig2mbpandneurotrophicfactorsandinhibitionofbaxandgfap |