The Impact of IL-18 and IFN-γ on Severity of Malaria and Quality of Treatment
Background & Objective: In human malaria, genotype of cytokines affect immune system. The impact of genotype -137G/C in IL-18 and +874A/T in IFN-γ on the severity of malaria and the quality of treatment – as factors to form asymptomatic persons in endemic regions- has been investigated.Materials...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2014-11-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-297-en.pdf |
| _version_ | 1797679682842263552 |
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| author | Gholam-Reza Ameri Kianoosh Malekzadeh Zahra Noor-Mohammadi Mohammad Shekari Habibollah Turki Hanieh Solaimani |
| author_facet | Gholam-Reza Ameri Kianoosh Malekzadeh Zahra Noor-Mohammadi Mohammad Shekari Habibollah Turki Hanieh Solaimani |
| author_sort | Gholam-Reza Ameri |
| collection | DOAJ |
| description | Background & Objective: In human malaria, genotype of cytokines affect immune
system. The impact of genotype -137G/C in IL-18 and +874A/T in IFN-γ on the severity
of malaria and the quality of treatment – as factors to form asymptomatic
persons in endemic regions- has been investigated.Materials & Methods: 100 patients and 102 healthy persons were evaluated
by Nested-PCR and thick blood film. IL-18(G-137C) and IFN- γ (A+874T) were
determined by Single Specific Primer-Polymerase Chain Reaction and ARMS-PCR
methods, respectively. The results were analyzed statistically.Results: In this research, it has been observed that allele-T in IFN- γ
gene and allele-C in IL-8 gene have protective effects against severity of
Malaria. In addition, it has clearly been found that development and growth
rate of plasmodium in µl of blood as well as anemia induced
malaria significantly reduced in carriers of these alleles (p<0.0001). This effect has also been observed in the quality
of the treatment in such a way that no parasite remained at the end of second
day of treatment in carriers of alleles IFN- γTT and IL-18CC. Conclusion: These findings issue the impact of immunogenetypes of patients on
treatment quality and asymptomatic patients and parasite reservoir as effective
factors in endemic malaria. Immunogenetypes of people may play as a remarkable
factor to control malaria in endemic areas. |
| first_indexed | 2024-03-11T23:18:26Z |
| format | Article |
| id | doaj.art-3da35c8fcfba4201bfcbeee78fbd7a6d |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:18:26Z |
| publishDate | 2014-11-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-3da35c8fcfba4201bfcbeee78fbd7a6d2023-09-20T21:17:45ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232014-11-0143284294The Impact of IL-18 and IFN-γ on Severity of Malaria and Quality of TreatmentGholam-Reza Ameri0Kianoosh Malekzadeh1Zahra Noor-Mohammadi2Mohammad Shekari3Habibollah Turki4Hanieh Solaimani5 Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. Department of Biology-Genetics, Faculty of Basic sciences, Science Research Branch, Islamic Azad University, Tehran, Iran. Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. Background & Objective: In human malaria, genotype of cytokines affect immune system. The impact of genotype -137G/C in IL-18 and +874A/T in IFN-γ on the severity of malaria and the quality of treatment – as factors to form asymptomatic persons in endemic regions- has been investigated.Materials & Methods: 100 patients and 102 healthy persons were evaluated by Nested-PCR and thick blood film. IL-18(G-137C) and IFN- γ (A+874T) were determined by Single Specific Primer-Polymerase Chain Reaction and ARMS-PCR methods, respectively. The results were analyzed statistically.Results: In this research, it has been observed that allele-T in IFN- γ gene and allele-C in IL-8 gene have protective effects against severity of Malaria. In addition, it has clearly been found that development and growth rate of plasmodium in µl of blood as well as anemia induced malaria significantly reduced in carriers of these alleles (p<0.0001). This effect has also been observed in the quality of the treatment in such a way that no parasite remained at the end of second day of treatment in carriers of alleles IFN- γTT and IL-18CC. Conclusion: These findings issue the impact of immunogenetypes of patients on treatment quality and asymptomatic patients and parasite reservoir as effective factors in endemic malaria. Immunogenetypes of people may play as a remarkable factor to control malaria in endemic areas.http://jabs.fums.ac.ir/article-1-297-en.pdfmalariail-18ifn-γ˛ genotypeparasite reservoir |
| spellingShingle | Gholam-Reza Ameri Kianoosh Malekzadeh Zahra Noor-Mohammadi Mohammad Shekari Habibollah Turki Hanieh Solaimani The Impact of IL-18 and IFN-γ on Severity of Malaria and Quality of Treatment Journal of Advanced Biomedical Sciences malaria il-18 ifn-γ˛ genotype parasite reservoir |
| title | The Impact of IL-18 and IFN-γ on Severity of Malaria and Quality of Treatment |
| title_full | The Impact of IL-18 and IFN-γ on Severity of Malaria and Quality of Treatment |
| title_fullStr | The Impact of IL-18 and IFN-γ on Severity of Malaria and Quality of Treatment |
| title_full_unstemmed | The Impact of IL-18 and IFN-γ on Severity of Malaria and Quality of Treatment |
| title_short | The Impact of IL-18 and IFN-γ on Severity of Malaria and Quality of Treatment |
| title_sort | impact of il 18 and ifn γ on severity of malaria and quality of treatment |
| topic | malaria il-18 ifn-γ˛ genotype parasite reservoir |
| url | http://jabs.fums.ac.ir/article-1-297-en.pdf |
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