Chromosomocentric approach to overcoming difficulties in implementation of international project Human Proteome
The international project Human Proteome (PHP), being a logical continuation of the project Human Genome, was started on September 23, 2010. In correspondence with the genocentric approach, the PHP aim is to prepare a catalogue of all human proteins and to decipher a network of their interactions. T...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2013-12-01
|
| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/11/Archakov_6_13.pdf |
| _version_ | 1797641809567940608 |
|---|---|
| author | A. I. Archakov V. G. Zgoda A. T. Kopylov1 S. N. Naryzhny A. L. Chernobrovkin E. A. Ponomarenko A. V. Lisitsa |
| author_facet | A. I. Archakov V. G. Zgoda A. T. Kopylov1 S. N. Naryzhny A. L. Chernobrovkin E. A. Ponomarenko A. V. Lisitsa |
| author_sort | A. I. Archakov |
| collection | DOAJ |
| description | The international project Human Proteome (PHP), being a logical continuation of the project Human Genome, was started on September 23, 2010. In correspondence with the genocentric approach, the PHP aim is to prepare a catalogue of all human proteins and to decipher a network of their interactions. The PHP implementation difficulties arise because the research subject itself – proteome – is much more complicated than genome. The major problem is the insufficient sensitivity of proteome methods that does not allow detecting low- and ultralow-copy proteins. Bad reproducibility of proteome methods and the lack of so-called “gold standard” is the second major complicacy in PHP implementation. The third problem is the dynamic character of proteome, its instability in time. The paper deals with possible variants of overcoming these complicacies, preventing from successful implementation of PHP. |
| first_indexed | 2024-03-11T13:51:03Z |
| format | Article |
| id | doaj.art-3da899ff14c54fee9a74150932f23b1b |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T13:51:03Z |
| publishDate | 2013-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-3da899ff14c54fee9a74150932f23b1b2023-11-02T09:03:56ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032013-12-0185681710.15407/ubj85.06.008Chromosomocentric approach to overcoming difficulties in implementation of international project Human ProteomeA. I. Archakov0 V. G. Zgoda1A. T. Kopylov12 S. N. Naryzhny3A. L. Chernobrovkin4E. A. Ponomarenko5A. V. Lisitsa6V. N. Orekhovich Scientific-Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences;V. N. Orekhovich Scientific-Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences;V. N. Orekhovich Scientific-Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences;V. N. Orekhovich Scientific-Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences;Petersburg Nuclear Physics Institute at National Research Centre Kurchatov InstituteV. N. Orekhovich Scientific-Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences;V. N. Orekhovich Scientific-Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences;V. N. Orekhovich Scientific-Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences;The international project Human Proteome (PHP), being a logical continuation of the project Human Genome, was started on September 23, 2010. In correspondence with the genocentric approach, the PHP aim is to prepare a catalogue of all human proteins and to decipher a network of their interactions. The PHP implementation difficulties arise because the research subject itself – proteome – is much more complicated than genome. The major problem is the insufficient sensitivity of proteome methods that does not allow detecting low- and ultralow-copy proteins. Bad reproducibility of proteome methods and the lack of so-called “gold standard” is the second major complicacy in PHP implementation. The third problem is the dynamic character of proteome, its instability in time. The paper deals with possible variants of overcoming these complicacies, preventing from successful implementation of PHP.http://ukrbiochemjournal.org/wp-content/uploads/2015/11/Archakov_6_13.pdfmass-spectrometric method of monitoring of multiple responsesproject Human Genomeproject Human Proteomesensitivity limit |
| spellingShingle | A. I. Archakov V. G. Zgoda A. T. Kopylov1 S. N. Naryzhny A. L. Chernobrovkin E. A. Ponomarenko A. V. Lisitsa Chromosomocentric approach to overcoming difficulties in implementation of international project Human Proteome The Ukrainian Biochemical Journal mass-spectrometric method of monitoring of multiple responses project Human Genome project Human Proteome sensitivity limit |
| title | Chromosomocentric approach to overcoming difficulties in implementation of international project Human Proteome |
| title_full | Chromosomocentric approach to overcoming difficulties in implementation of international project Human Proteome |
| title_fullStr | Chromosomocentric approach to overcoming difficulties in implementation of international project Human Proteome |
| title_full_unstemmed | Chromosomocentric approach to overcoming difficulties in implementation of international project Human Proteome |
| title_short | Chromosomocentric approach to overcoming difficulties in implementation of international project Human Proteome |
| title_sort | chromosomocentric approach to overcoming difficulties in implementation of international project human proteome |
| topic | mass-spectrometric method of monitoring of multiple responses project Human Genome project Human Proteome sensitivity limit |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2015/11/Archakov_6_13.pdf |
| work_keys_str_mv | AT aiarchakov chromosomocentricapproachtoovercomingdifficultiesinimplementationofinternationalprojecthumanproteome AT vgzgoda chromosomocentricapproachtoovercomingdifficultiesinimplementationofinternationalprojecthumanproteome AT atkopylov1 chromosomocentricapproachtoovercomingdifficultiesinimplementationofinternationalprojecthumanproteome AT snnaryzhny chromosomocentricapproachtoovercomingdifficultiesinimplementationofinternationalprojecthumanproteome AT alchernobrovkin chromosomocentricapproachtoovercomingdifficultiesinimplementationofinternationalprojecthumanproteome AT eaponomarenko chromosomocentricapproachtoovercomingdifficultiesinimplementationofinternationalprojecthumanproteome AT avlisitsa chromosomocentricapproachtoovercomingdifficultiesinimplementationofinternationalprojecthumanproteome |