Genotype-by-Sequencing Analysis of Mutations and Recombination in Pepper Progeny of Gamma-Irradiated Gametophytes
The irradiation of dry seeds is the most widely-used irradiation method for improving seed-propagated crops; however, the irradiation of other tissues also has useful effects. The irradiation of plant reproductive organs, rather than seeds, for mutation breeding has advantages, such as producing non...
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| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/1/144 |
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| author | Yeong Deuk Jo Han Sol Kang Hong-Il Choi Jin-Baek Kim |
| author_facet | Yeong Deuk Jo Han Sol Kang Hong-Il Choi Jin-Baek Kim |
| author_sort | Yeong Deuk Jo |
| collection | DOAJ |
| description | The irradiation of dry seeds is the most widely-used irradiation method for improving seed-propagated crops; however, the irradiation of other tissues also has useful effects. The irradiation of plant reproductive organs, rather than seeds, for mutation breeding has advantages, such as producing non-chimeric progeny. However, the mutation frequency and spectrum produced using this method have not been analyzed on a genome-wide level. We performed a genotype-by-sequencing analysis to determine the frequencies of single-base substitutions and small (1–2 bp) insertions and deletions in hot pepper (<i>Capsicum annuum</i> L.) plants derived from crosses using gamma-irradiated female or male gametophytes. The progeny of irradiated gametophytes showed similar or higher DNA mutation frequencies, which were dependent on the irradiation dose and irradiated tissue, and less biased single base substitutions than progeny of irradiated seeds. These characteristics were expected to be beneficial for development of mutation population with a high frequency of small DNA mutations and performing reverse-genetics-based mutation screening. We also examined the possible use of this irradiation method in manipulating the meiotic recombination frequency; however, no statistically significant increase was detected. Our results provide useful information for further research and breeding using irradiated gametophytes. |
| first_indexed | 2024-03-09T05:05:51Z |
| format | Article |
| id | doaj.art-3da973ef40304c6c8f3b24154b7ec5e3 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T05:05:51Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-3da973ef40304c6c8f3b24154b7ec5e32023-12-03T12:54:49ZengMDPI AGPlants2223-77472021-01-0110114410.3390/plants10010144Genotype-by-Sequencing Analysis of Mutations and Recombination in Pepper Progeny of Gamma-Irradiated GametophytesYeong Deuk Jo0Han Sol Kang1Hong-Il Choi2Jin-Baek Kim3Radiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, KoreaRadiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, KoreaRadiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, KoreaRadiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, KoreaThe irradiation of dry seeds is the most widely-used irradiation method for improving seed-propagated crops; however, the irradiation of other tissues also has useful effects. The irradiation of plant reproductive organs, rather than seeds, for mutation breeding has advantages, such as producing non-chimeric progeny. However, the mutation frequency and spectrum produced using this method have not been analyzed on a genome-wide level. We performed a genotype-by-sequencing analysis to determine the frequencies of single-base substitutions and small (1–2 bp) insertions and deletions in hot pepper (<i>Capsicum annuum</i> L.) plants derived from crosses using gamma-irradiated female or male gametophytes. The progeny of irradiated gametophytes showed similar or higher DNA mutation frequencies, which were dependent on the irradiation dose and irradiated tissue, and less biased single base substitutions than progeny of irradiated seeds. These characteristics were expected to be beneficial for development of mutation population with a high frequency of small DNA mutations and performing reverse-genetics-based mutation screening. We also examined the possible use of this irradiation method in manipulating the meiotic recombination frequency; however, no statistically significant increase was detected. Our results provide useful information for further research and breeding using irradiated gametophytes.https://www.mdpi.com/2223-7747/10/1/144mutation breedinggamma-raysgametophyterecombinationgenotype-by-sequencing (GBS) |
| spellingShingle | Yeong Deuk Jo Han Sol Kang Hong-Il Choi Jin-Baek Kim Genotype-by-Sequencing Analysis of Mutations and Recombination in Pepper Progeny of Gamma-Irradiated Gametophytes Plants mutation breeding gamma-rays gametophyte recombination genotype-by-sequencing (GBS) |
| title | Genotype-by-Sequencing Analysis of Mutations and Recombination in Pepper Progeny of Gamma-Irradiated Gametophytes |
| title_full | Genotype-by-Sequencing Analysis of Mutations and Recombination in Pepper Progeny of Gamma-Irradiated Gametophytes |
| title_fullStr | Genotype-by-Sequencing Analysis of Mutations and Recombination in Pepper Progeny of Gamma-Irradiated Gametophytes |
| title_full_unstemmed | Genotype-by-Sequencing Analysis of Mutations and Recombination in Pepper Progeny of Gamma-Irradiated Gametophytes |
| title_short | Genotype-by-Sequencing Analysis of Mutations and Recombination in Pepper Progeny of Gamma-Irradiated Gametophytes |
| title_sort | genotype by sequencing analysis of mutations and recombination in pepper progeny of gamma irradiated gametophytes |
| topic | mutation breeding gamma-rays gametophyte recombination genotype-by-sequencing (GBS) |
| url | https://www.mdpi.com/2223-7747/10/1/144 |
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