Diagnostic accuracy of Panbio rapid antigen tests on oropharyngeal swabs for detection of SARS-CoV-2.
<h4>Background</h4>Antigen-detecting rapid diagnostic tests (Ag-RDTs) for the detection of SARS-CoV-2 offer new opportunities for testing in the context of the COVID-19 pandemic. Nasopharyngeal swabs (NPS) are the reference sample type, but oropharyngeal swabs (OPS) may be a more accepta...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0253321 |
| _version_ | 1818929658257735680 |
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| author | Marie Thérèse Ngo Nsoga Ilona Kronig Francisco Javier Perez Rodriguez Pascale Sattonnet-Roche Diogo Da Silva Javan Helbling Jilian A Sacks Margaretha de Vos Erik Boehm Angèle Gayet-Ageron Alice Berger Frédérique Jacquerioz-Bausch François Chappuis Laurent Kaiser Manuel Schibler Adriana Renzoni Isabella Eckerle |
| author_facet | Marie Thérèse Ngo Nsoga Ilona Kronig Francisco Javier Perez Rodriguez Pascale Sattonnet-Roche Diogo Da Silva Javan Helbling Jilian A Sacks Margaretha de Vos Erik Boehm Angèle Gayet-Ageron Alice Berger Frédérique Jacquerioz-Bausch François Chappuis Laurent Kaiser Manuel Schibler Adriana Renzoni Isabella Eckerle |
| author_sort | Marie Thérèse Ngo Nsoga |
| collection | DOAJ |
| description | <h4>Background</h4>Antigen-detecting rapid diagnostic tests (Ag-RDTs) for the detection of SARS-CoV-2 offer new opportunities for testing in the context of the COVID-19 pandemic. Nasopharyngeal swabs (NPS) are the reference sample type, but oropharyngeal swabs (OPS) may be a more acceptable sample type in some patients.<h4>Methods</h4>We conducted a prospective study in a single screening center to assess the diagnostic performance of the Panbio™ COVID-19 Ag Rapid Test (Abbott) on OPS compared with reverse-transcription quantitative PCR (RT-qPCR) using NPS during the second pandemic wave in Switzerland.<h4>Results</h4>402 outpatients were enrolled in a COVID-19 screening center, of whom 168 (41.8%) had a positive RT-qPCR test. The oropharyngeal Ag-RDT clinical sensitivity compared to nasopharyngeal RT-qPCR was 81% (95%CI: 74.2-86.6). Two false positives were noted out of the 234 RT-qPCR negative individuals, which resulted in a clinical specificity of 99.1% (95%CI: 96.9-99.9) for the Ag-RDT. For cycle threshold values ≤ 26.7 (≥ 1E6 SARS-CoV-2 genomes copies/mL, a presumed cut-off for infectious virus), 96.3% sensitivity (95%CI: 90.7-99.0%) was obtained with the Ag-RDT using OPS.<h4>Interpretation</h4>Based on our findings, the diagnostic performance of the Panbio™ Covid-19 RDT with OPS samples, if taken by a trained person and high requirements regarding quality of the specimen, meet the criteria required by the WHO for Ag-RDTs (sensitivity ≥80% and specificity ≥97%) in a high incidence setting in symptomatic individuals. |
| first_indexed | 2024-12-20T03:48:18Z |
| format | Article |
| id | doaj.art-3dac66c35ce74638a995fe7b08f65566 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T03:48:18Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3dac66c35ce74638a995fe7b08f655662022-12-21T19:54:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01166e025332110.1371/journal.pone.0253321Diagnostic accuracy of Panbio rapid antigen tests on oropharyngeal swabs for detection of SARS-CoV-2.Marie Thérèse Ngo NsogaIlona KronigFrancisco Javier Perez RodriguezPascale Sattonnet-RocheDiogo Da SilvaJavan HelblingJilian A SacksMargaretha de VosErik BoehmAngèle Gayet-AgeronAlice BergerFrédérique Jacquerioz-BauschFrançois ChappuisLaurent KaiserManuel SchiblerAdriana RenzoniIsabella Eckerle<h4>Background</h4>Antigen-detecting rapid diagnostic tests (Ag-RDTs) for the detection of SARS-CoV-2 offer new opportunities for testing in the context of the COVID-19 pandemic. Nasopharyngeal swabs (NPS) are the reference sample type, but oropharyngeal swabs (OPS) may be a more acceptable sample type in some patients.<h4>Methods</h4>We conducted a prospective study in a single screening center to assess the diagnostic performance of the Panbio™ COVID-19 Ag Rapid Test (Abbott) on OPS compared with reverse-transcription quantitative PCR (RT-qPCR) using NPS during the second pandemic wave in Switzerland.<h4>Results</h4>402 outpatients were enrolled in a COVID-19 screening center, of whom 168 (41.8%) had a positive RT-qPCR test. The oropharyngeal Ag-RDT clinical sensitivity compared to nasopharyngeal RT-qPCR was 81% (95%CI: 74.2-86.6). Two false positives were noted out of the 234 RT-qPCR negative individuals, which resulted in a clinical specificity of 99.1% (95%CI: 96.9-99.9) for the Ag-RDT. For cycle threshold values ≤ 26.7 (≥ 1E6 SARS-CoV-2 genomes copies/mL, a presumed cut-off for infectious virus), 96.3% sensitivity (95%CI: 90.7-99.0%) was obtained with the Ag-RDT using OPS.<h4>Interpretation</h4>Based on our findings, the diagnostic performance of the Panbio™ Covid-19 RDT with OPS samples, if taken by a trained person and high requirements regarding quality of the specimen, meet the criteria required by the WHO for Ag-RDTs (sensitivity ≥80% and specificity ≥97%) in a high incidence setting in symptomatic individuals.https://doi.org/10.1371/journal.pone.0253321 |
| spellingShingle | Marie Thérèse Ngo Nsoga Ilona Kronig Francisco Javier Perez Rodriguez Pascale Sattonnet-Roche Diogo Da Silva Javan Helbling Jilian A Sacks Margaretha de Vos Erik Boehm Angèle Gayet-Ageron Alice Berger Frédérique Jacquerioz-Bausch François Chappuis Laurent Kaiser Manuel Schibler Adriana Renzoni Isabella Eckerle Diagnostic accuracy of Panbio rapid antigen tests on oropharyngeal swabs for detection of SARS-CoV-2. PLoS ONE |
| title | Diagnostic accuracy of Panbio rapid antigen tests on oropharyngeal swabs for detection of SARS-CoV-2. |
| title_full | Diagnostic accuracy of Panbio rapid antigen tests on oropharyngeal swabs for detection of SARS-CoV-2. |
| title_fullStr | Diagnostic accuracy of Panbio rapid antigen tests on oropharyngeal swabs for detection of SARS-CoV-2. |
| title_full_unstemmed | Diagnostic accuracy of Panbio rapid antigen tests on oropharyngeal swabs for detection of SARS-CoV-2. |
| title_short | Diagnostic accuracy of Panbio rapid antigen tests on oropharyngeal swabs for detection of SARS-CoV-2. |
| title_sort | diagnostic accuracy of panbio rapid antigen tests on oropharyngeal swabs for detection of sars cov 2 |
| url | https://doi.org/10.1371/journal.pone.0253321 |
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