Ectopic transient overexpression of facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells
Limitation in cell sources for autologous cell therapy has been a recent focus in stem cell therapy and tissue engineering. Among various research advances, direct conversion, or transdifferentiation, is a notable and feasible strategy for the generation and acquirement of wanted cell source. So far...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-03-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731420909208 |
| _version_ | 1819063000376541184 |
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| author | Seung Hyun L Kim Seunghun S Lee Inseon Kim Janet Kwon Song Kwon Taegeun Bae Junho Hur Hwajin Lee Nathaniel S Hwang |
| author_facet | Seung Hyun L Kim Seunghun S Lee Inseon Kim Janet Kwon Song Kwon Taegeun Bae Junho Hur Hwajin Lee Nathaniel S Hwang |
| author_sort | Seung Hyun L Kim |
| collection | DOAJ |
| description | Limitation in cell sources for autologous cell therapy has been a recent focus in stem cell therapy and tissue engineering. Among various research advances, direct conversion, or transdifferentiation, is a notable and feasible strategy for the generation and acquirement of wanted cell source. So far, utilizing cell transdifferentiation technology in tissue engineering was mainly restricted at achieving single wanted cell type from diverse cell types with high efficiency. However, regeneration of a complete tissue always requires multiple cell types which poses an intrinsic complexity. In this study, enhanced osteogenic differentiation was achieved by transient ectopic expression of octamer-binding transcription factor 4 ( OCT-4 ) gene followed by bone morphogenetic protein 4 treatment on human umbilical vein endothelial cells. OCT-4 transfection and bone morphogenetic protein 4 treatment resulted in enhanced expression of osteogenic markers such as core-binding factor alpha 1, alkaline phosphatase, and collagen 1 compared with bone morphogenetic protein 4 treatment alone. Furthermore, we employed gelatin-heparin cryogel in cranial defect model for in vivo bone formation. Micro-computed tomography and histological analysis of in vivo samples showed that OCT-4 transfection followed by bone morphogenetic protein 4 treatment resulted in efficient transdifferentiation of endothelial cells to osteogenic cells. These results suggest that the combination of OCT-4 and bone morphogenetic protein 4 on endothelial cells would be a reliable multicellular transdifferentiation model which could be applied for bone tissue engineering. |
| first_indexed | 2024-12-21T15:07:43Z |
| format | Article |
| id | doaj.art-3db085025e654e2dada2b17bcd641af4 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-21T15:07:43Z |
| publishDate | 2020-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-3db085025e654e2dada2b17bcd641af42022-12-21T18:59:22ZengSAGE PublishingJournal of Tissue Engineering2041-73142020-03-011110.1177/2041731420909208Ectopic transient overexpression of facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cellsSeung Hyun L Kim0Seunghun S Lee1Inseon Kim2Janet Kwon3Song Kwon4Taegeun Bae5Junho Hur6Hwajin Lee7Nathaniel S Hwang8Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, Republic of KoreaInterdisciplinary Program in Bioengineering, Seoul National University, Seoul, Republic of KoreaSchool of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul, Republic of KoreaInterdisciplinary Program in Bioengineering, Seoul National University, Seoul, Republic of KoreaSchool of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul, Republic of KoreaBioMAX/N-Bio Institute, Seoul National University, Seoul, Republic of KoreaDepartment of Medicine, Kyung Hee University, Seoul, Republic of KoreaSchool of Dentistry, Seoul National University, Seoul, Republic of KoreaBioMAX/N-Bio Institute, Seoul National University, Seoul, Republic of KoreaLimitation in cell sources for autologous cell therapy has been a recent focus in stem cell therapy and tissue engineering. Among various research advances, direct conversion, or transdifferentiation, is a notable and feasible strategy for the generation and acquirement of wanted cell source. So far, utilizing cell transdifferentiation technology in tissue engineering was mainly restricted at achieving single wanted cell type from diverse cell types with high efficiency. However, regeneration of a complete tissue always requires multiple cell types which poses an intrinsic complexity. In this study, enhanced osteogenic differentiation was achieved by transient ectopic expression of octamer-binding transcription factor 4 ( OCT-4 ) gene followed by bone morphogenetic protein 4 treatment on human umbilical vein endothelial cells. OCT-4 transfection and bone morphogenetic protein 4 treatment resulted in enhanced expression of osteogenic markers such as core-binding factor alpha 1, alkaline phosphatase, and collagen 1 compared with bone morphogenetic protein 4 treatment alone. Furthermore, we employed gelatin-heparin cryogel in cranial defect model for in vivo bone formation. Micro-computed tomography and histological analysis of in vivo samples showed that OCT-4 transfection followed by bone morphogenetic protein 4 treatment resulted in efficient transdifferentiation of endothelial cells to osteogenic cells. These results suggest that the combination of OCT-4 and bone morphogenetic protein 4 on endothelial cells would be a reliable multicellular transdifferentiation model which could be applied for bone tissue engineering.https://doi.org/10.1177/2041731420909208 |
| spellingShingle | Seung Hyun L Kim Seunghun S Lee Inseon Kim Janet Kwon Song Kwon Taegeun Bae Junho Hur Hwajin Lee Nathaniel S Hwang Ectopic transient overexpression of facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells Journal of Tissue Engineering |
| title | Ectopic transient overexpression of facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells |
| title_full | Ectopic transient overexpression of facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells |
| title_fullStr | Ectopic transient overexpression of facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells |
| title_full_unstemmed | Ectopic transient overexpression of facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells |
| title_short | Ectopic transient overexpression of facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells |
| title_sort | ectopic transient overexpression of facilitates bmp4 induced osteogenic transdifferentiation of human umbilical vein endothelial cells |
| url | https://doi.org/10.1177/2041731420909208 |
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