Geno/cytotoxicty and Apoptotic Properties of Phenolic Compounds from the Seeds of Dorema Glabrum Fisch. C.A
Introduction: Dorema glabrum (Apiaceae) is a rare and monocarpic species distributed in Transcaucasia and North West of Iran. We aimed to explore anticancer potency of bioactive compounds from the seeds of Dorma glabrum. Methods: Methanol extract was subjected to phytochemical investigation using no...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2014-12-01
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| Series: | BioImpacts |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/BI/PDF/BI-4-191.pdf |
| Summary: | Introduction: Dorema glabrum
(Apiaceae) is a rare and monocarpic species distributed in
Transcaucasia and North West of Iran. We aimed to explore anticancer
potency of bioactive compounds from the seeds of Dorma glabrum.
Methods: Methanol extract was subjected to phytochemical investigation using
normal phase Sep-pak and reversed-phase HPLC, and cytotoxic effect of
isolated compounds on CAOV-4 cell line was evaluated. Furthermore,
Annexin V/PI staining and comet assay were used to study genotoxicity of
compounds.
Results: Diglucosyl caffeoyl ester (1), Glucopyranosylcaffeic acid (2) and
skimmin (3), were identified. MTT cytotoxicity assay showed growth
inhibition of CAOV-4 cells due to treatment with compunds (1), (2) and
(3) with an IC50 of 99.7, 87.3 and 70.03 µg/ml at 48 h, respectively.
Annexin V-FITC/PI staining showed occurrence of early/late apoptosis in
the (1)-treated cells, while (2)-and (3)-treated cells necrosis/late
apoptosis was dominant event. Single/double strands DNA breakages were
observed by comet assay in all treatments.
Conclusion: This work provides sufficient information about anti-cancer properties of the diglucosyl caffeoyl ester from the seeds of D. glabrum. |
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| ISSN: | 2228-5652 2228-5660 |