Cymodocea serrulata-capped silver nanoparticles for battling human lung cancer, breast cancer, hepatic cancer: Optimization by full factorial design and in vitro cytotoxicity evaluation
In recent times, there has been growing interest in nanoparticles (NPs) synthesized through biological means due to their ease of production and their potential applications in the field of biology. This study presents an environmentally friendly method for the biogenic synthesis of silver nanoparti...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402307247X |
| _version_ | 1797669763399286784 |
|---|---|
| author | S. Venmani Mookkandi Palsamy Kesavan Srinivasan Ayyanaar N. Muniyappan |
| author_facet | S. Venmani Mookkandi Palsamy Kesavan Srinivasan Ayyanaar N. Muniyappan |
| author_sort | S. Venmani |
| collection | DOAJ |
| description | In recent times, there has been growing interest in nanoparticles (NPs) synthesized through biological means due to their ease of production and their potential applications in the field of biology. This study presents an environmentally friendly method for the biogenic synthesis of silver nanoparticles (AgNPs) using the leaf extract of Cymodocea serrulata as both a reducing agent and a capping agent. Various physico-chemical and microscopic techniques were employed to comprehensively characterize the biogenically produced AgNPs. The results of these characterization studies confirmed the formation of spherical, stable, and crystalline AgNPs with an average size of 30.5 ± 2.5 nm. Furthermore, the antibacterial assessment revealed the remarkable antibacterial properties of these biogenically synthesized Ag NPs, even at exceedingly low concentrations ranging from 50 to 100 μg/mL. The IC50 values for the biogenically synthesized AgNPs against different human cancer cell lines, such as A549, MDA-MB-231, HepG2, and MCF-7, were determined to be 93.4 ± 4.5, 82.5 ± 3.7, 87.6 ± 4.1, and 57.3 ± 2.5 μg/mL, respectively. Most notably, the biogenically synthesized Ag NPs exhibited significant anti-inflammatory activity, as evidenced by their IC50 value of 30.08 ± 1.4 μg/mL, as assessed through the HRBC membrane stabilization method. These in vitro findings strongly suggest that AgNPs fabricated through biogenic processes using Cymodocea serrulata leaf extract hold promise as potential therapeutic candidates for combating bacterial infections, cancer, and inflammatory conditions. |
| first_indexed | 2024-03-11T20:49:17Z |
| format | Article |
| id | doaj.art-3db0d58205e24a5cb9b9ee051ef1e154 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:49:17Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-3db0d58205e24a5cb9b9ee051ef1e1542023-10-01T06:02:21ZengElsevierHeliyon2405-84402023-09-0199e20039Cymodocea serrulata-capped silver nanoparticles for battling human lung cancer, breast cancer, hepatic cancer: Optimization by full factorial design and in vitro cytotoxicity evaluationS. Venmani0Mookkandi Palsamy Kesavan1Srinivasan Ayyanaar2N. Muniyappan3Department of Chemistry, Saraswathi Narayanan College, Madurai, 625 022, Tamil Nadu, IndiaDepartment of Chemistry, Hajee Karutha Rowther Howdia College, Uthamapalayam, 625 533, Tamil Nadu, IndiaDepartment of Chemistry, Syed Ammal Arts and Science College, Pullankudi, 623 513, Ramanathapuram, Tamilnadu, IndiaDepartment of Chemistry, Saraswathi Narayanan College, Madurai, 625 022, Tamil Nadu, India; Corresponding author.In recent times, there has been growing interest in nanoparticles (NPs) synthesized through biological means due to their ease of production and their potential applications in the field of biology. This study presents an environmentally friendly method for the biogenic synthesis of silver nanoparticles (AgNPs) using the leaf extract of Cymodocea serrulata as both a reducing agent and a capping agent. Various physico-chemical and microscopic techniques were employed to comprehensively characterize the biogenically produced AgNPs. The results of these characterization studies confirmed the formation of spherical, stable, and crystalline AgNPs with an average size of 30.5 ± 2.5 nm. Furthermore, the antibacterial assessment revealed the remarkable antibacterial properties of these biogenically synthesized Ag NPs, even at exceedingly low concentrations ranging from 50 to 100 μg/mL. The IC50 values for the biogenically synthesized AgNPs against different human cancer cell lines, such as A549, MDA-MB-231, HepG2, and MCF-7, were determined to be 93.4 ± 4.5, 82.5 ± 3.7, 87.6 ± 4.1, and 57.3 ± 2.5 μg/mL, respectively. Most notably, the biogenically synthesized Ag NPs exhibited significant anti-inflammatory activity, as evidenced by their IC50 value of 30.08 ± 1.4 μg/mL, as assessed through the HRBC membrane stabilization method. These in vitro findings strongly suggest that AgNPs fabricated through biogenic processes using Cymodocea serrulata leaf extract hold promise as potential therapeutic candidates for combating bacterial infections, cancer, and inflammatory conditions.http://www.sciencedirect.com/science/article/pii/S240584402307247XSilver nanoparticlesBiogenic synthesisAntibacterial activityAnticancer activityAnti-inflammatory activity |
| spellingShingle | S. Venmani Mookkandi Palsamy Kesavan Srinivasan Ayyanaar N. Muniyappan Cymodocea serrulata-capped silver nanoparticles for battling human lung cancer, breast cancer, hepatic cancer: Optimization by full factorial design and in vitro cytotoxicity evaluation Heliyon Silver nanoparticles Biogenic synthesis Antibacterial activity Anticancer activity Anti-inflammatory activity |
| title | Cymodocea serrulata-capped silver nanoparticles for battling human lung cancer, breast cancer, hepatic cancer: Optimization by full factorial design and in vitro cytotoxicity evaluation |
| title_full | Cymodocea serrulata-capped silver nanoparticles for battling human lung cancer, breast cancer, hepatic cancer: Optimization by full factorial design and in vitro cytotoxicity evaluation |
| title_fullStr | Cymodocea serrulata-capped silver nanoparticles for battling human lung cancer, breast cancer, hepatic cancer: Optimization by full factorial design and in vitro cytotoxicity evaluation |
| title_full_unstemmed | Cymodocea serrulata-capped silver nanoparticles for battling human lung cancer, breast cancer, hepatic cancer: Optimization by full factorial design and in vitro cytotoxicity evaluation |
| title_short | Cymodocea serrulata-capped silver nanoparticles for battling human lung cancer, breast cancer, hepatic cancer: Optimization by full factorial design and in vitro cytotoxicity evaluation |
| title_sort | cymodocea serrulata capped silver nanoparticles for battling human lung cancer breast cancer hepatic cancer optimization by full factorial design and in vitro cytotoxicity evaluation |
| topic | Silver nanoparticles Biogenic synthesis Antibacterial activity Anticancer activity Anti-inflammatory activity |
| url | http://www.sciencedirect.com/science/article/pii/S240584402307247X |
| work_keys_str_mv | AT svenmani cymodoceaserrulatacappedsilvernanoparticlesforbattlinghumanlungcancerbreastcancerhepaticcanceroptimizationbyfullfactorialdesignandinvitrocytotoxicityevaluation AT mookkandipalsamykesavan cymodoceaserrulatacappedsilvernanoparticlesforbattlinghumanlungcancerbreastcancerhepaticcanceroptimizationbyfullfactorialdesignandinvitrocytotoxicityevaluation AT srinivasanayyanaar cymodoceaserrulatacappedsilvernanoparticlesforbattlinghumanlungcancerbreastcancerhepaticcanceroptimizationbyfullfactorialdesignandinvitrocytotoxicityevaluation AT nmuniyappan cymodoceaserrulatacappedsilvernanoparticlesforbattlinghumanlungcancerbreastcancerhepaticcanceroptimizationbyfullfactorialdesignandinvitrocytotoxicityevaluation |