Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan
The current study was undertaken to characterize the RRDR rpoB gene mutations among the rifampicin-resistant Mycobacterium tuberculosis (MTB) isolates from Pakistan. Rifampicin mutation patterns were analyzed by using PCR followed by rpoB gene sequencing. Among the 1080 referred TB cases, 63 (6%) we...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2014;volume=3;issue=3;spage=173;epage=177;aulast=Qazi |
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| author | Obaidullah Qazi Hazir Rahman Zarfishan Tahir Muhammad Qasim Sajid Khan Aftab Ahmad Anjum Tahir Yaqub Muhammad Tayyab Nawab Ali Sehrish Firyal |
| author_facet | Obaidullah Qazi Hazir Rahman Zarfishan Tahir Muhammad Qasim Sajid Khan Aftab Ahmad Anjum Tahir Yaqub Muhammad Tayyab Nawab Ali Sehrish Firyal |
| author_sort | Obaidullah Qazi |
| collection | DOAJ |
| description | The current study was undertaken to characterize the RRDR rpoB gene mutations among the rifampicin-resistant Mycobacterium tuberculosis (MTB) isolates from Pakistan. Rifampicin mutation patterns were analyzed by using PCR followed by rpoB gene sequencing. Among the 1080 referred TB cases, 63 (6%) were resistant against at least one first-line TB drug. Out of these 63 resistant isolates, 24 isolates (38%) were found to be resistant to isoniazid and rifampicin. Sequence analysis of multidrug-resistant tuberculosis (MDR-TB) isolates detected a single mutation in the RRDR region of the rpoB gene at codon 531, 516, 512, 528 and 533; however, 5 MDR-TB isolates lack any mutation in the RRDR region. A double mutation was observed in 1 MDR-TB isolate at codon 512 and 516 which are reported for the first time from Pakistan. Moreover, in 1 isolate a novel silent mutation was observed at codon 528. Further studies about these mutations may be helpful in the development of diagnostic tools for the detection of MTB in a high TB endemic area like Pakistan. |
| first_indexed | 2024-12-16T06:35:22Z |
| format | Article |
| id | doaj.art-3db22a65ae024994a46864db1d4a4ce4 |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-12-16T06:35:22Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-3db22a65ae024994a46864db1d4a4ce42022-12-21T22:40:48ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2014-01-013317317710.1016/j.ijmyco.2014.06.004Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from PakistanObaidullah QaziHazir RahmanZarfishan TahirMuhammad QasimSajid KhanAftab Ahmad AnjumTahir YaqubMuhammad TayyabNawab AliSehrish FiryalThe current study was undertaken to characterize the RRDR rpoB gene mutations among the rifampicin-resistant Mycobacterium tuberculosis (MTB) isolates from Pakistan. Rifampicin mutation patterns were analyzed by using PCR followed by rpoB gene sequencing. Among the 1080 referred TB cases, 63 (6%) were resistant against at least one first-line TB drug. Out of these 63 resistant isolates, 24 isolates (38%) were found to be resistant to isoniazid and rifampicin. Sequence analysis of multidrug-resistant tuberculosis (MDR-TB) isolates detected a single mutation in the RRDR region of the rpoB gene at codon 531, 516, 512, 528 and 533; however, 5 MDR-TB isolates lack any mutation in the RRDR region. A double mutation was observed in 1 MDR-TB isolate at codon 512 and 516 which are reported for the first time from Pakistan. Moreover, in 1 isolate a novel silent mutation was observed at codon 528. Further studies about these mutations may be helpful in the development of diagnostic tools for the detection of MTB in a high TB endemic area like Pakistan.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2014;volume=3;issue=3;spage=173;epage=177;aulast=QaziMycobacterium tuberculosisMultidrug-resistant isolatesSequencingRRDR rpoB gene mutations |
| spellingShingle | Obaidullah Qazi Hazir Rahman Zarfishan Tahir Muhammad Qasim Sajid Khan Aftab Ahmad Anjum Tahir Yaqub Muhammad Tayyab Nawab Ali Sehrish Firyal Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan International Journal of Mycobacteriology Mycobacterium tuberculosis Multidrug-resistant isolates Sequencing RRDR rpoB gene mutations |
| title | Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan |
| title_full | Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan |
| title_fullStr | Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan |
| title_full_unstemmed | Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan |
| title_short | Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan |
| title_sort | mutation pattern in rifampicin resistance determining region of rpob gene in multidrug resistant mycobacterium tuberculosis isolates from pakistan |
| topic | Mycobacterium tuberculosis Multidrug-resistant isolates Sequencing RRDR rpoB gene mutations |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2014;volume=3;issue=3;spage=173;epage=177;aulast=Qazi |
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