PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.Br
Phytochemical and hypoglycemic effects of two varieties of Gymnema sylvestre were studied. The leaves of Big-leaf (MCL) and the small-leaf (ZMGL) varieties were grown in Uka Tarsadia University, Bardoli, India. Water, ethanol, methanol, petroleum ether, haxane and chloroform were used for phytochemi...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2016-04-01
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| Series: | Indonesian Journal of Pharmacy |
| Subjects: | |
| Online Access: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1059 |
| _version_ | 1818129657420054528 |
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| author | Ramar Krishnamurthy David Adedayo Animasaun R. T. Patel Rajashekhar Ingalhalli |
| author_facet | Ramar Krishnamurthy David Adedayo Animasaun R. T. Patel Rajashekhar Ingalhalli |
| author_sort | Ramar Krishnamurthy |
| collection | DOAJ |
| description | Phytochemical and hypoglycemic effects of two varieties of Gymnema sylvestre were studied. The leaves of Big-leaf (MCL) and the small-leaf (ZMGL) varieties were grown in Uka Tarsadia University, Bardoli, India. Water, ethanol, methanol, petroleum ether, haxane and chloroform were used for phytochemical extraction from the dried leaves. Gymnemic acid was extracted and purified from the two varieties by Thin Layer Chromatography (TLC). Four groups of matured whisker rats (six per group) were induced with high-glucose level. Three groups were treated with standard drug Glibenclamide (5mg/kg), gymnemic acid extracts from MCL and ZMDL respectively while the fourth group served as experimental control. Hypoglycemic activities of extracts were evaluated using animals’ blood sample. Result showed water and methanol are best solvents for phytochemical extraction from the plant and the yield is higher in ZMGL than MCL. Also, gymnemic acid yield varied. From the results, hypoglycemic activities from the animals’ blood revealed that treatments from both MCL and ZMGL are not significantly different from standard drug Glibenclamide. The study concluded that water or methanol is appropriate solvent for phytochemical extraction from G. sylvestre leaves and extract from both lines of the plant could be utilized to reduce blood glucose level |
| first_indexed | 2024-12-11T07:52:38Z |
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| id | doaj.art-3db25c320cd24253b95aa43cf27c292c |
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| issn | 2338-9427 2338-9486 |
| language | English |
| last_indexed | 2024-12-11T07:52:38Z |
| publishDate | 2016-04-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Pharmacy |
| spelling | doaj.art-3db25c320cd24253b95aa43cf27c292c2022-12-22T01:15:17ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862016-04-01272596510.14499/indonesianjpharm27iss2pp59PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.BrRamar Krishnamurthy0David Adedayo Animasaun1R. T. Patel2Rajashekhar Ingalhalli3C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Bardoli, Surat, Gujarat, IndiaDepartment of Plant Biology, University Of Ilorin, P.M.B 1515, Ilorin, Kwara State, NigeriaC.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Bardoli, Surat, Gujarat, IndiaC.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Bardoli, Surat, Gujarat, IndiaPhytochemical and hypoglycemic effects of two varieties of Gymnema sylvestre were studied. The leaves of Big-leaf (MCL) and the small-leaf (ZMGL) varieties were grown in Uka Tarsadia University, Bardoli, India. Water, ethanol, methanol, petroleum ether, haxane and chloroform were used for phytochemical extraction from the dried leaves. Gymnemic acid was extracted and purified from the two varieties by Thin Layer Chromatography (TLC). Four groups of matured whisker rats (six per group) were induced with high-glucose level. Three groups were treated with standard drug Glibenclamide (5mg/kg), gymnemic acid extracts from MCL and ZMDL respectively while the fourth group served as experimental control. Hypoglycemic activities of extracts were evaluated using animals’ blood sample. Result showed water and methanol are best solvents for phytochemical extraction from the plant and the yield is higher in ZMGL than MCL. Also, gymnemic acid yield varied. From the results, hypoglycemic activities from the animals’ blood revealed that treatments from both MCL and ZMGL are not significantly different from standard drug Glibenclamide. The study concluded that water or methanol is appropriate solvent for phytochemical extraction from G. sylvestre leaves and extract from both lines of the plant could be utilized to reduce blood glucose levelhttp://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1059phytochemical extractionextraction solventsgymnemic acidhypoglycemic effects |
| spellingShingle | Ramar Krishnamurthy David Adedayo Animasaun R. T. Patel Rajashekhar Ingalhalli PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.Br Indonesian Journal of Pharmacy phytochemical extraction extraction solvents gymnemic acid hypoglycemic effects |
| title | PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.Br |
| title_full | PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.Br |
| title_fullStr | PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.Br |
| title_full_unstemmed | PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.Br |
| title_short | PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.Br |
| title_sort | phytochemical constituents and hypoglycemic effect of gymnemic acid extracts from big and small leaf varieties of gymnema sylvestre r br |
| topic | phytochemical extraction extraction solvents gymnemic acid hypoglycemic effects |
| url | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1059 |
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