Role and molecular mechanism of APOBEC3B in the development and progression of gastric cancer
Gastric cancer is a common malignant tumor with a high mortality rate. Abnormal APOBEC3B (apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3B) expression increases tumor susceptibility. However, the exact molecular mechanism of APOBEC3B expression in the development of gastric cancer...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024004894 |
| _version_ | 1827360873776152576 |
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| author | Nana Su Erle Zhou Min Cui Hong Li Shuhua Wu Qian Zhang Zhang Cao |
| author_facet | Nana Su Erle Zhou Min Cui Hong Li Shuhua Wu Qian Zhang Zhang Cao |
| author_sort | Nana Su |
| collection | DOAJ |
| description | Gastric cancer is a common malignant tumor with a high mortality rate. Abnormal APOBEC3B (apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3B) expression increases tumor susceptibility. However, the exact molecular mechanism of APOBEC3B expression in the development of gastric cancer is still unknown. We investigated the effect of APOBEC3B on the malignant biological behavior of gastric cancer cells and discussed the role of APOBEC3B in the development and progression of gastric cancer. APOBEC3B protein levels were measured in 161 gastric cancer samples using western blotting and immunohistochemistry. Both in vitro and in vivo assays were performed, and molecules were analyzed using bioinformatics analysis and western blotting. APOBEC3B was overexpressed in gastric cancer. Moreover, APOBEC3B significantly enhanced cell proliferation in vitro and tumorigenicity in vivo. Regarding the underlying mechanism, APOBEC3B promoted the proliferation of gastric cancer cells by upregulating P53, MCM2 (minichromosome maintenance protein 2), and cyclin D1. Our results suggest that APOBEC3B is involved in cancer progression, providing a new theoretical basis for the prevention and treatment of gastric cancer. |
| first_indexed | 2024-03-08T06:54:46Z |
| format | Article |
| id | doaj.art-3db321d2d1534ea897e8c9615e388aad |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T06:54:46Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-3db321d2d1534ea897e8c9615e388aad2024-02-03T06:37:33ZengElsevierHeliyon2405-84402024-01-01102e24458Role and molecular mechanism of APOBEC3B in the development and progression of gastric cancerNana Su0Erle Zhou1Min Cui2Hong Li3Shuhua Wu4Qian Zhang5Zhang Cao6Department of Pathology, Binzhou Medical University Hospital, Binzhou, 256603, ChinaDepartment of Pathology, Binzhou Medical University Hospital, Binzhou, 256603, ChinaDepartment of Pediatrics, Binzhou City People's Hospital, Binzhou, 256600, ChinaDepartment of Pathology, Binzhou Medical University Hospital, Binzhou, 256603, ChinaDepartment of Pathology, Binzhou Medical University Hospital, Binzhou, 256603, ChinaDepartment of Pathology, Binzhou Medical University Hospital, Binzhou, 256603, China; Corresponding author.Department of Pathology, Binzhou Medical University Hospital, Binzhou, 256603, China; Corresponding author.Gastric cancer is a common malignant tumor with a high mortality rate. Abnormal APOBEC3B (apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3B) expression increases tumor susceptibility. However, the exact molecular mechanism of APOBEC3B expression in the development of gastric cancer is still unknown. We investigated the effect of APOBEC3B on the malignant biological behavior of gastric cancer cells and discussed the role of APOBEC3B in the development and progression of gastric cancer. APOBEC3B protein levels were measured in 161 gastric cancer samples using western blotting and immunohistochemistry. Both in vitro and in vivo assays were performed, and molecules were analyzed using bioinformatics analysis and western blotting. APOBEC3B was overexpressed in gastric cancer. Moreover, APOBEC3B significantly enhanced cell proliferation in vitro and tumorigenicity in vivo. Regarding the underlying mechanism, APOBEC3B promoted the proliferation of gastric cancer cells by upregulating P53, MCM2 (minichromosome maintenance protein 2), and cyclin D1. Our results suggest that APOBEC3B is involved in cancer progression, providing a new theoretical basis for the prevention and treatment of gastric cancer.http://www.sciencedirect.com/science/article/pii/S2405844024004894APOBEC3BCyclin D1Gastric cancerMCM2P53 |
| spellingShingle | Nana Su Erle Zhou Min Cui Hong Li Shuhua Wu Qian Zhang Zhang Cao Role and molecular mechanism of APOBEC3B in the development and progression of gastric cancer Heliyon APOBEC3B Cyclin D1 Gastric cancer MCM2 P53 |
| title | Role and molecular mechanism of APOBEC3B in the development and progression of gastric cancer |
| title_full | Role and molecular mechanism of APOBEC3B in the development and progression of gastric cancer |
| title_fullStr | Role and molecular mechanism of APOBEC3B in the development and progression of gastric cancer |
| title_full_unstemmed | Role and molecular mechanism of APOBEC3B in the development and progression of gastric cancer |
| title_short | Role and molecular mechanism of APOBEC3B in the development and progression of gastric cancer |
| title_sort | role and molecular mechanism of apobec3b in the development and progression of gastric cancer |
| topic | APOBEC3B Cyclin D1 Gastric cancer MCM2 P53 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024004894 |
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