Constitutive Expression of <i>Arabidopsis</i> Senescence Associated Gene 101 in <i>Brachypodium distachyon</i> Enhances Resistance to <i>Puccinia brachypodii</i> and <i>Magnaporthe oryzae</i>
<i>Brachypodium distachyon</i>, as an effective model of cereal grains, is susceptible to most destructive cereal pathogens. <i>Senescence associated gene 101</i> (<i>SAG101</i>) has been studied extensively in <i>Arabidopsis</i>. <i>SAG101</i...
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MDPI AG
2020-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/10/1316 |
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| author | Ning Wang Na Song Zejun Tang Xiaojie Wang Zhensheng Kang Liangying Dai Bing Wang |
| author_facet | Ning Wang Na Song Zejun Tang Xiaojie Wang Zhensheng Kang Liangying Dai Bing Wang |
| author_sort | Ning Wang |
| collection | DOAJ |
| description | <i>Brachypodium distachyon</i>, as an effective model of cereal grains, is susceptible to most destructive cereal pathogens. <i>Senescence associated gene 101</i> (<i>SAG101</i>) has been studied extensively in <i>Arabidopsis</i>. <i>SAG101</i> is one of the important regulators of plant immunity. However, no homologous genes of <i>AtSAG101</i> were found in <i>B. distachyon</i>. In this study, the <i>AtSAG101</i> gene was transformed into <i>B. distachyon.</i> Three transgenic plant lines containing the <i>AtSAG101</i> gene were confirmed by PCR and GUS gene activity. There were fewer <i>Puccinia brachypodii</i> urediospores in the <i>AtSAG101</i>-overexpressing plants compared to wild type plants. <i>P. brachypodii</i> biomass was obviously decreased in <i>AtSAG101</i> transgenic plants. The length of infection hyphae and infection unit areas of <i>P. brachypodii</i> were significantly limited in transgenic plants. Moreover, there were small lesions in <i>AtSAG101</i> transgenic plants challenged by <i>Magnaporthe oryzae</i>. Salicylic acid accumulation was significantly increased, which led to elevated <i>pathogenesis-related</i> gene expression in transgenic <i>B. distachyon</i> inoculated by <i>P. brachypodii</i> or <i>M. oryzae</i> compared to wild type plants. These results were consistent with infected phenotypes. Overexpression of <i>AtSAG101</i> in <i>B. distachyon</i> caused resistance to <i>M. oryzae</i> and <i>P. brachypodii</i>. These results suggest that <i>AtSAG101</i> could regulate plant resistance in <i>B. distachyon</i>. |
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| last_indexed | 2024-03-10T15:49:34Z |
| publishDate | 2020-10-01 |
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| spelling | doaj.art-3db898b869574006a3f45f536bdc1c872023-11-20T16:10:01ZengMDPI AGPlants2223-77472020-10-01910131610.3390/plants9101316Constitutive Expression of <i>Arabidopsis</i> Senescence Associated Gene 101 in <i>Brachypodium distachyon</i> Enhances Resistance to <i>Puccinia brachypodii</i> and <i>Magnaporthe oryzae</i>Ning Wang0Na Song1Zejun Tang2Xiaojie Wang3Zhensheng Kang4Liangying Dai5Bing Wang6Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests and College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests and College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests and College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests and College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests and College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan, China<i>Brachypodium distachyon</i>, as an effective model of cereal grains, is susceptible to most destructive cereal pathogens. <i>Senescence associated gene 101</i> (<i>SAG101</i>) has been studied extensively in <i>Arabidopsis</i>. <i>SAG101</i> is one of the important regulators of plant immunity. However, no homologous genes of <i>AtSAG101</i> were found in <i>B. distachyon</i>. In this study, the <i>AtSAG101</i> gene was transformed into <i>B. distachyon.</i> Three transgenic plant lines containing the <i>AtSAG101</i> gene were confirmed by PCR and GUS gene activity. There were fewer <i>Puccinia brachypodii</i> urediospores in the <i>AtSAG101</i>-overexpressing plants compared to wild type plants. <i>P. brachypodii</i> biomass was obviously decreased in <i>AtSAG101</i> transgenic plants. The length of infection hyphae and infection unit areas of <i>P. brachypodii</i> were significantly limited in transgenic plants. Moreover, there were small lesions in <i>AtSAG101</i> transgenic plants challenged by <i>Magnaporthe oryzae</i>. Salicylic acid accumulation was significantly increased, which led to elevated <i>pathogenesis-related</i> gene expression in transgenic <i>B. distachyon</i> inoculated by <i>P. brachypodii</i> or <i>M. oryzae</i> compared to wild type plants. These results were consistent with infected phenotypes. Overexpression of <i>AtSAG101</i> in <i>B. distachyon</i> caused resistance to <i>M. oryzae</i> and <i>P. brachypodii</i>. These results suggest that <i>AtSAG101</i> could regulate plant resistance in <i>B. distachyon</i>.https://www.mdpi.com/2223-7747/9/10/1316Senescence Associated Gene 101salicylic acidoverexpression<i>Puccinia brachypodii</i>light microscopy |
| spellingShingle | Ning Wang Na Song Zejun Tang Xiaojie Wang Zhensheng Kang Liangying Dai Bing Wang Constitutive Expression of <i>Arabidopsis</i> Senescence Associated Gene 101 in <i>Brachypodium distachyon</i> Enhances Resistance to <i>Puccinia brachypodii</i> and <i>Magnaporthe oryzae</i> Plants Senescence Associated Gene 101 salicylic acid overexpression <i>Puccinia brachypodii</i> light microscopy |
| title | Constitutive Expression of <i>Arabidopsis</i> Senescence Associated Gene 101 in <i>Brachypodium distachyon</i> Enhances Resistance to <i>Puccinia brachypodii</i> and <i>Magnaporthe oryzae</i> |
| title_full | Constitutive Expression of <i>Arabidopsis</i> Senescence Associated Gene 101 in <i>Brachypodium distachyon</i> Enhances Resistance to <i>Puccinia brachypodii</i> and <i>Magnaporthe oryzae</i> |
| title_fullStr | Constitutive Expression of <i>Arabidopsis</i> Senescence Associated Gene 101 in <i>Brachypodium distachyon</i> Enhances Resistance to <i>Puccinia brachypodii</i> and <i>Magnaporthe oryzae</i> |
| title_full_unstemmed | Constitutive Expression of <i>Arabidopsis</i> Senescence Associated Gene 101 in <i>Brachypodium distachyon</i> Enhances Resistance to <i>Puccinia brachypodii</i> and <i>Magnaporthe oryzae</i> |
| title_short | Constitutive Expression of <i>Arabidopsis</i> Senescence Associated Gene 101 in <i>Brachypodium distachyon</i> Enhances Resistance to <i>Puccinia brachypodii</i> and <i>Magnaporthe oryzae</i> |
| title_sort | constitutive expression of i arabidopsis i senescence associated gene 101 in i brachypodium distachyon i enhances resistance to i puccinia brachypodii i and i magnaporthe oryzae i |
| topic | Senescence Associated Gene 101 salicylic acid overexpression <i>Puccinia brachypodii</i> light microscopy |
| url | https://www.mdpi.com/2223-7747/9/10/1316 |
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