Tear Liquid for Predictive Diagnosis of Alzheimer’s Disease
The common approach of the diagnosis of Alzheimer’s Disease (AD) is made with an analysis of the cerebrospinal fluid or the study of retinal fundus and the plaques formation through optical corneal tomography (OCT), or more simply with a fundus camera. Tears analysis is widely discussed in literatur...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2571-841X/4/3/26 |
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| author | Salvatore Del Prete Daniela Marasco Rosalaura Sabetta Antonio Del Prete Federica Zito Marino Renato Franco Salvatore Troisi Mario Troisi Gilda Cennamo |
| author_facet | Salvatore Del Prete Daniela Marasco Rosalaura Sabetta Antonio Del Prete Federica Zito Marino Renato Franco Salvatore Troisi Mario Troisi Gilda Cennamo |
| author_sort | Salvatore Del Prete |
| collection | DOAJ |
| description | The common approach of the diagnosis of Alzheimer’s Disease (AD) is made with an analysis of the cerebrospinal fluid or the study of retinal fundus and the plaques formation through optical corneal tomography (OCT), or more simply with a fundus camera. Tears analysis is widely discussed in literature as an essential method to describe molecular and biochemical alterations in different diseases. The aim of our study was the identification with immunocytochemistry of Amyloid Beta-42 in tears from patients with or without familiarity for Alzheimer Disease, in order to make the diagnosis earlier and more accessible compared to other invasive methods. Our study was performed on tears from three phenotypically healthy subjects: two of them were Caucasian with Alzheimer familiarity (48 and 55 years old) and the other one was Asian without Alzheimer familiarity (45 years old) and affected by an adenoviral keratoconjunctivitis at the moment of withdrawal. Tear samples were collected from eye fornix and were examinated by immunocytochemistry (ICC) assay using anti-Amyloid Beta X-42 antibody. Two out of three tears samples showed positive Amyloid Beta-42. Considering that our patients were phenotypically healthy, the identification of Amyloid Beta-42 by ICC could be a candidable method to make the diagnosis of the disease earlier and more accessible and available then other current and invasive methods and it could be a candidate for a screening method too. |
| first_indexed | 2024-03-10T07:14:16Z |
| format | Article |
| id | doaj.art-3db8be417e964e438c58ee88e6501667 |
| institution | Directory Open Access Journal |
| issn | 2571-841X |
| language | English |
| last_indexed | 2024-03-10T07:14:16Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Reports |
| spelling | doaj.art-3db8be417e964e438c58ee88e65016672023-11-22T15:08:31ZengMDPI AGReports2571-841X2021-08-01432610.3390/reports4030026Tear Liquid for Predictive Diagnosis of Alzheimer’s DiseaseSalvatore Del Prete0Daniela Marasco1Rosalaura Sabetta2Antonio Del Prete3Federica Zito Marino4Renato Franco5Salvatore Troisi6Mario Troisi7Gilda Cennamo8Service Biotech s.r.l., 80132 Napoli, ItalyService Biotech s.r.l., 80132 Napoli, ItalyDipartimento di Salute Mentale e Fisica e Medicina Preventiva, Università degli Studi della Campania Luigi Vanvitelli, 80100 Naples, ItalyDepartment of Neurosciences and Reproductive and Dentistry Sciences, School of Medicine and Surgery, University of Naples Federico II, 80100 Naples, ItalyDipartimento di Salute Mentale e Fisica e Medicina Preventiva, Università degli Studi della Campania Luigi Vanvitelli, 80100 Naples, ItalyDipartimento di Salute Mentale e Fisica e Medicina Preventiva, Università degli Studi della Campania Luigi Vanvitelli, 80100 Naples, ItalyOphthalmologic Unit, Salerno Hospital University, 84100 Salerno, ItalyOphthalmologic Unit, Salerno Hospital University, 84100 Salerno, ItalyDepartment of Neurosciences and Reproductive and Dentistry Sciences, School of Medicine and Surgery, University of Naples Federico II, 80100 Naples, ItalyThe common approach of the diagnosis of Alzheimer’s Disease (AD) is made with an analysis of the cerebrospinal fluid or the study of retinal fundus and the plaques formation through optical corneal tomography (OCT), or more simply with a fundus camera. Tears analysis is widely discussed in literature as an essential method to describe molecular and biochemical alterations in different diseases. The aim of our study was the identification with immunocytochemistry of Amyloid Beta-42 in tears from patients with or without familiarity for Alzheimer Disease, in order to make the diagnosis earlier and more accessible compared to other invasive methods. Our study was performed on tears from three phenotypically healthy subjects: two of them were Caucasian with Alzheimer familiarity (48 and 55 years old) and the other one was Asian without Alzheimer familiarity (45 years old) and affected by an adenoviral keratoconjunctivitis at the moment of withdrawal. Tear samples were collected from eye fornix and were examinated by immunocytochemistry (ICC) assay using anti-Amyloid Beta X-42 antibody. Two out of three tears samples showed positive Amyloid Beta-42. Considering that our patients were phenotypically healthy, the identification of Amyloid Beta-42 by ICC could be a candidable method to make the diagnosis of the disease earlier and more accessible and available then other current and invasive methods and it could be a candidate for a screening method too.https://www.mdpi.com/2571-841X/4/3/26Alzheimerdiagnostic methodconjunctival swabAlzheimer familiarityAmyloid Beta-42 |
| spellingShingle | Salvatore Del Prete Daniela Marasco Rosalaura Sabetta Antonio Del Prete Federica Zito Marino Renato Franco Salvatore Troisi Mario Troisi Gilda Cennamo Tear Liquid for Predictive Diagnosis of Alzheimer’s Disease Reports Alzheimer diagnostic method conjunctival swab Alzheimer familiarity Amyloid Beta-42 |
| title | Tear Liquid for Predictive Diagnosis of Alzheimer’s Disease |
| title_full | Tear Liquid for Predictive Diagnosis of Alzheimer’s Disease |
| title_fullStr | Tear Liquid for Predictive Diagnosis of Alzheimer’s Disease |
| title_full_unstemmed | Tear Liquid for Predictive Diagnosis of Alzheimer’s Disease |
| title_short | Tear Liquid for Predictive Diagnosis of Alzheimer’s Disease |
| title_sort | tear liquid for predictive diagnosis of alzheimer s disease |
| topic | Alzheimer diagnostic method conjunctival swab Alzheimer familiarity Amyloid Beta-42 |
| url | https://www.mdpi.com/2571-841X/4/3/26 |
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