Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress
Abstract Introduction The basis of orthodontic tooth movement (OTM) is the reconstruction of periodontal tissue under stress. Increasing the speed of OTM has always been the focus of attention. Objectives Periodontal ligament stem cells (PDLSCs) are direct effector cells of mechanical force, but the...
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-022-03055-z |
| _version_ | 1811315273743990784 |
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| author | Li Wang Hong Liang Bingjing Sun Jing Mi Xianqin Tong Yuhui Wang Meihua Chen Liming Yu Jie Pan Shangfeng Liu Yan-Jun Liu Yuehua Liu |
| author_facet | Li Wang Hong Liang Bingjing Sun Jing Mi Xianqin Tong Yuhui Wang Meihua Chen Liming Yu Jie Pan Shangfeng Liu Yan-Jun Liu Yuehua Liu |
| author_sort | Li Wang |
| collection | DOAJ |
| description | Abstract Introduction The basis of orthodontic tooth movement (OTM) is the reconstruction of periodontal tissue under stress. Increasing the speed of OTM has always been the focus of attention. Objectives Periodontal ligament stem cells (PDLSCs) are direct effector cells of mechanical force, but the mechanism by which PDLSCs sense mechanical stimuli is unclear. Methods Human PDLSCs (hPDLSCs) were analyzed in the presence or absence of force loading with the Flexcell loading system in vitro. Then, periodontal tissues were analyzed after mechanical stimulation in vivo. In addition, cells in a confined microenvironment were analyzed to observe changes in the cytoskeleton and migration. Finally, TRPC6−/− mice were used to further verify the effect of TRPC6. After force application, the OTM distance, bone marrow density (BMD), TRPC6 and COL1 expression, and TRAP staining were evaluated in periodontal tissues. Results RNA sequencing (RNA-seq) and western blot analyses revealed that TRPC6 was important during mechanical force application to hPDLSCs. Appropriate mechanical force application also induced TRPC6 activation in the OTM model and the confined microenvironment. Under a slightly confined microenvironment, treatment with the TRPC6 inhibitor SKF96365 and TRPC6 knockout decreased the migration speed of hPDLSCs and mouse bone marrow mesenchymal stem cells (mBMSCs). In addition, TRPC6−/− mice showed lower OTM distances and reduced osteogenic and osteoclastic differentiation. Conclusion In summary, TRPC6 activation in PDLSCs mediated by appropriate mechanical force application contributes to periodontal tissue reconstruction. Graphical abstract PDLSCs modulate periodontal tissue remodeling under appropriate mechanical stimulation through TRPC6; however, under excessive stress, alveolar bone and tooth roots are readily absorbed. Under this condition, environmental factors play a leading role, and the regulatory effect of TRPC6 is not obvious. |
| first_indexed | 2024-04-13T11:28:28Z |
| format | Article |
| id | doaj.art-3db9433576024a12882c8916f5e02aa4 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-04-13T11:28:28Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-3db9433576024a12882c8916f5e02aa42022-12-22T02:48:38ZengBMCStem Cell Research & Therapy1757-65122022-08-0113111510.1186/s13287-022-03055-zRole of TRPC6 in periodontal tissue reconstruction mediated by appropriate stressLi Wang0Hong Liang1Bingjing Sun2Jing Mi3Xianqin Tong4Yuhui Wang5Meihua Chen6Liming Yu7Jie Pan8Shangfeng Liu9Yan-Jun Liu10Yuehua Liu11Department of Orthodontics, Shanghai Stomatological Hospital, Fudan UniversityShanghai Key Laboratory of Medical Epigenetics, International Co-Laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Department of Systems Biology for Medicine, Fudan UniversityDepartment of Orthodontics, Shanghai Stomatological Hospital, Fudan UniversityDepartment of Orthodontics, Shanghai Stomatological Hospital, Fudan UniversityDepartment of Orthodontics, Shanghai Stomatological Hospital, Fudan UniversityDepartment of Orthodontics, Shanghai Stomatological Hospital, Fudan UniversityDepartment of Orthodontics, Shanghai Stomatological Hospital, Fudan UniversityDepartment of Orthodontics, Shanghai Stomatological Hospital, Fudan UniversityDepartment of Orthodontics, Shanghai Stomatological Hospital, Fudan UniversityShanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Shanghai Stomatological Hospital, Fudan UniversityShanghai Key Laboratory of Medical Epigenetics, International Co-Laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Department of Systems Biology for Medicine, Fudan UniversityDepartment of Orthodontics, Shanghai Stomatological Hospital, Fudan UniversityAbstract Introduction The basis of orthodontic tooth movement (OTM) is the reconstruction of periodontal tissue under stress. Increasing the speed of OTM has always been the focus of attention. Objectives Periodontal ligament stem cells (PDLSCs) are direct effector cells of mechanical force, but the mechanism by which PDLSCs sense mechanical stimuli is unclear. Methods Human PDLSCs (hPDLSCs) were analyzed in the presence or absence of force loading with the Flexcell loading system in vitro. Then, periodontal tissues were analyzed after mechanical stimulation in vivo. In addition, cells in a confined microenvironment were analyzed to observe changes in the cytoskeleton and migration. Finally, TRPC6−/− mice were used to further verify the effect of TRPC6. After force application, the OTM distance, bone marrow density (BMD), TRPC6 and COL1 expression, and TRAP staining were evaluated in periodontal tissues. Results RNA sequencing (RNA-seq) and western blot analyses revealed that TRPC6 was important during mechanical force application to hPDLSCs. Appropriate mechanical force application also induced TRPC6 activation in the OTM model and the confined microenvironment. Under a slightly confined microenvironment, treatment with the TRPC6 inhibitor SKF96365 and TRPC6 knockout decreased the migration speed of hPDLSCs and mouse bone marrow mesenchymal stem cells (mBMSCs). In addition, TRPC6−/− mice showed lower OTM distances and reduced osteogenic and osteoclastic differentiation. Conclusion In summary, TRPC6 activation in PDLSCs mediated by appropriate mechanical force application contributes to periodontal tissue reconstruction. Graphical abstract PDLSCs modulate periodontal tissue remodeling under appropriate mechanical stimulation through TRPC6; however, under excessive stress, alveolar bone and tooth roots are readily absorbed. Under this condition, environmental factors play a leading role, and the regulatory effect of TRPC6 is not obvious.https://doi.org/10.1186/s13287-022-03055-zTRPC6Mechanical forcePeriodontal ligament stem cellsTRPC6 −/− miceA confined microenvironment |
| spellingShingle | Li Wang Hong Liang Bingjing Sun Jing Mi Xianqin Tong Yuhui Wang Meihua Chen Liming Yu Jie Pan Shangfeng Liu Yan-Jun Liu Yuehua Liu Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress Stem Cell Research & Therapy TRPC6 Mechanical force Periodontal ligament stem cells TRPC6 −/− mice A confined microenvironment |
| title | Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress |
| title_full | Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress |
| title_fullStr | Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress |
| title_full_unstemmed | Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress |
| title_short | Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress |
| title_sort | role of trpc6 in periodontal tissue reconstruction mediated by appropriate stress |
| topic | TRPC6 Mechanical force Periodontal ligament stem cells TRPC6 −/− mice A confined microenvironment |
| url | https://doi.org/10.1186/s13287-022-03055-z |
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