The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c Mice
Background: TGF-β has long been considered as the main inducer of Tregs in tumor microenvironment and is the reason for the aberrant number of Tregs in tumor-bearing individuals. Recently, it has been suggested that the enzyme arginase I is able to mediate the induction of Tregs in a TGF-β-independe...
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2020-07-01
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| Series: | Iranian Biomedical Journal |
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| Online Access: | http://ibj.pasteur.ac.ir/article-1-3041-en.html |
| _version_ | 1819103810603188224 |
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| author | Hani Mosayebzadeh Roshan Seyed-Hosein Abtahi-Eivary Hassan Shojaee-Mend Alireza Mohammadzadeh Zahra Bahari Sani |
| author_facet | Hani Mosayebzadeh Roshan Seyed-Hosein Abtahi-Eivary Hassan Shojaee-Mend Alireza Mohammadzadeh Zahra Bahari Sani |
| author_sort | Hani Mosayebzadeh Roshan |
| collection | DOAJ |
| description | Background: TGF-β has long been considered as the main inducer of Tregs in tumor microenvironment and is the reason for the aberrant number of Tregs in tumor-bearing individuals. Recently, it has been suggested that the enzyme arginase I is able to mediate the induction of Tregs in a TGF-β-independent fashion. The recombinant WW2/WW3 domains from smad ubiquitination regulatory factor 2 molecule was demonstrated to increase TGF-β signaling while reducing arginase I gene expression. In this study, we aimed to examine the effects of this recombinant protein on CD4+CD25+/CD4+ proportion in the spleen of 4T1 mammary carcinoma-bearing BALB/c mice. Methods: Flow cytometry was used to evaluate CD4+CD25+ spleen cell populations of the tumor-bearing mice that received WW2/WW3 protein treatment and those of the control group. Results: The results indicated a significant rise in CD4+CD25+/CD4+ ratio, along with an average increase in tumor mass of the subjects that underwent protein treatment. Conclusion: It can be inferred that the heightened CD4+CD25+/CD4+ proportion in the spleen of protein-treated tumor-bearing mice can be the result of the increased TGF-β signaling despite the reduced arginase I expression. |
| first_indexed | 2024-12-22T01:56:23Z |
| format | Article |
| id | doaj.art-3db944a787ee4c65ad4175365e1d26ba |
| institution | Directory Open Access Journal |
| issn | 1028-852X 2008-823X |
| language | English |
| last_indexed | 2024-12-22T01:56:23Z |
| publishDate | 2020-07-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Iranian Biomedical Journal |
| spelling | doaj.art-3db944a787ee4c65ad4175365e1d26ba2022-12-21T18:42:47ZengPasteur Institute of IranIranian Biomedical Journal1028-852X2008-823X2020-07-01244214219The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c MiceHani Mosayebzadeh Roshan0Seyed-Hosein Abtahi-Eivary1Hassan Shojaee-Mend2Alireza Mohammadzadeh3Zahra Bahari Sani4 Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran Department of Medical Laboratory Sciences, Faculty of Paramedical Sciences, Gonabad University of Medical Sciences, Gonabad, Iran Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran Department of Medical Laboratory Sciences, Faculty of Paramedical Sciences, Gonabad University of Medical Sciences, Gonabad, Iran Department of Medical Laboratory Sciences, Faculty of Paramedical Sciences, Gonabad University of Medical Sciences, Gonabad, Iran Background: TGF-β has long been considered as the main inducer of Tregs in tumor microenvironment and is the reason for the aberrant number of Tregs in tumor-bearing individuals. Recently, it has been suggested that the enzyme arginase I is able to mediate the induction of Tregs in a TGF-β-independent fashion. The recombinant WW2/WW3 domains from smad ubiquitination regulatory factor 2 molecule was demonstrated to increase TGF-β signaling while reducing arginase I gene expression. In this study, we aimed to examine the effects of this recombinant protein on CD4+CD25+/CD4+ proportion in the spleen of 4T1 mammary carcinoma-bearing BALB/c mice. Methods: Flow cytometry was used to evaluate CD4+CD25+ spleen cell populations of the tumor-bearing mice that received WW2/WW3 protein treatment and those of the control group. Results: The results indicated a significant rise in CD4+CD25+/CD4+ ratio, along with an average increase in tumor mass of the subjects that underwent protein treatment. Conclusion: It can be inferred that the heightened CD4+CD25+/CD4+ proportion in the spleen of protein-treated tumor-bearing mice can be the result of the increased TGF-β signaling despite the reduced arginase I expression.http://ibj.pasteur.ac.ir/article-1-3041-en.htmlarginasetransforming growth factor-ꞵww domains |
| spellingShingle | Hani Mosayebzadeh Roshan Seyed-Hosein Abtahi-Eivary Hassan Shojaee-Mend Alireza Mohammadzadeh Zahra Bahari Sani The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c Mice Iranian Biomedical Journal arginase transforming growth factor-ꞵ ww domains |
| title | The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c Mice |
| title_full | The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c Mice |
| title_fullStr | The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c Mice |
| title_full_unstemmed | The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c Mice |
| title_short | The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c Mice |
| title_sort | effects of ww2 ww3 domains of smurf2 molecule on cd4 cd25 cd4 proportion in spleen of 4t1 tumor bearing balb c mice |
| topic | arginase transforming growth factor-ꞵ ww domains |
| url | http://ibj.pasteur.ac.ir/article-1-3041-en.html |
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