Application of the Se NPs-Chitosan molecular complex for the correction of selenium deficiency in rats model
Selenium is an integral component of vital biologically active compounds of the human body. Currently, the population of many countries is characterized by selenium deficiency. In this regard, many preparations of inorganic and organic forms of selenium have been developed. Nevertheless, it is evid...
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| Format: | Article |
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HACCP Consulting
2023-06-01
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| Series: | Potravinarstvo |
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| Online Access: | https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1871 |
| _version_ | 1797810176055574528 |
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| author | Marina Verevkina Vadim Goncharov Evgeny Nesmeyanov Olga Kamalova Igor Baklanov Alexander Pokhilko Anzhela Nagapetova Petr Miroshnichenko |
| author_facet | Marina Verevkina Vadim Goncharov Evgeny Nesmeyanov Olga Kamalova Igor Baklanov Alexander Pokhilko Anzhela Nagapetova Petr Miroshnichenko |
| author_sort | Marina Verevkina |
| collection | DOAJ |
| description |
Selenium is an integral component of vital biologically active compounds of the human body. Currently, the population of many countries is characterized by selenium deficiency. In this regard, many preparations of inorganic and organic forms of selenium have been developed. Nevertheless, it is evident that the most effective solution to the problem is to enrich the diet with bioavailable forms of selenium. Thus, this work aimed to synthesize and study the antioxidant and immunomodulatory effects of the molecular complex of selenium nanoparticles (Se NPs) and chitosan in laboratory rats with induced hyposelenosis. During the experiment with animals, we found that as a result of 70-day consumption of food with a low selenium content, rats develop an alimentary selenium deficiency state, as evidenced by a significant decrease in the content of this trace element in control group rats to 48.2 ±6.71 µg/kg versus 149.3 ±21.63 µg/kg in intact animals. Course, administration of the molecular complex Se NPs- Chitosan to rats of the experimental group, contributed to the replenishment of selenium deficiency: its concentration in the blood of animals was 96.6 ±3.57 µg/kg. Thus, in animals of the control group, there was a decrease in the total number of lymphocytes by 2.7 times, T-lymphocytes – by 1.8 times, and B-lymphocytes – by 2.3 times compared with similar data in intact animals. In the context of hyposelenosis, it is worth mentioning that there was a slight increase in the content of T-helper cells and cytotoxic T-lymphocytes. The synthesized Se NPs – Chitosan complex administration during hyposelenosis demonstrated a notable immunomodulatory effect by restoring the body's immune response indicators. Thus, the total number of lymphocytes increased by 3 times, T-lymphocytes – by 1.9 times, and B-lymphocytes – by 2 times. The number of T-helper cells and cytotoxic T-lymphocytes increased by 1.9 times compared to the group of intact animals and 1.6 times compared to selenium-deficient rats. Thus, the course introduction of the molecular complex Se NPs – Chitosan against the background of selenium deficiency was accompanied by inhibition of free radical oxidation processes, activation of the antioxidant system and restoration of the immune status of the organism of laboratory animals.
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| first_indexed | 2024-03-13T07:04:15Z |
| format | Article |
| id | doaj.art-3dbab90c493b4b8bb1c7f9767104beb0 |
| institution | Directory Open Access Journal |
| issn | 1337-0960 |
| language | English |
| last_indexed | 2024-03-13T07:04:15Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Potravinarstvo |
| spelling | doaj.art-3dbab90c493b4b8bb1c7f9767104beb02023-06-06T13:09:43ZengHACCP ConsultingPotravinarstvo1337-09602023-06-011710.5219/1871Application of the Se NPs-Chitosan molecular complex for the correction of selenium deficiency in rats modelMarina Verevkina0Vadim Goncharov1Evgeny Nesmeyanov2Olga Kamalova3Igor Baklanov4Alexander Pokhilko5Anzhela Nagapetova6Petr Miroshnichenko7Stavropol State Agrarian University, Zootechnicheskiy lane, 12, 355035, Stavropol, Russia, Tel.: +7-989-98-99-48Don State Technical University, 41/1, Kulakova avenue, 355035, Stavropol, Russia, Tel.: +79187592273, Don State Technical University, 1, Gagarin Square, 344003, Rostov-on-Don, Russia, Tel.: +79034068839Rostov State Medical University, 29, Nakhichevan lane, 344022, Rostov-on-Don, Russia, Tel.: +79185061337North Caucasus Federal University, Pushkina Street, 1, 355009, Stavropol, Russia, Tel.: +7-962-002-76-13Armavir State Pedagogical University, Roza Luxembourg street, 159, 352901, Armavir, Krasnodar region, Russia, Tel.: +7-963-383-53-78Armavir State Pedagogical University, Roza Luxembourg street, 159, 352901, Armavir, Krasnodar region, Russia, Tel.: +7-989-973-75-78Kuban State Agrarian University, Kalinina street, 13, 350044, Krasnodar, Russia, Tel.: +79184470155 Selenium is an integral component of vital biologically active compounds of the human body. Currently, the population of many countries is characterized by selenium deficiency. In this regard, many preparations of inorganic and organic forms of selenium have been developed. Nevertheless, it is evident that the most effective solution to the problem is to enrich the diet with bioavailable forms of selenium. Thus, this work aimed to synthesize and study the antioxidant and immunomodulatory effects of the molecular complex of selenium nanoparticles (Se NPs) and chitosan in laboratory rats with induced hyposelenosis. During the experiment with animals, we found that as a result of 70-day consumption of food with a low selenium content, rats develop an alimentary selenium deficiency state, as evidenced by a significant decrease in the content of this trace element in control group rats to 48.2 ±6.71 µg/kg versus 149.3 ±21.63 µg/kg in intact animals. Course, administration of the molecular complex Se NPs- Chitosan to rats of the experimental group, contributed to the replenishment of selenium deficiency: its concentration in the blood of animals was 96.6 ±3.57 µg/kg. Thus, in animals of the control group, there was a decrease in the total number of lymphocytes by 2.7 times, T-lymphocytes – by 1.8 times, and B-lymphocytes – by 2.3 times compared with similar data in intact animals. In the context of hyposelenosis, it is worth mentioning that there was a slight increase in the content of T-helper cells and cytotoxic T-lymphocytes. The synthesized Se NPs – Chitosan complex administration during hyposelenosis demonstrated a notable immunomodulatory effect by restoring the body's immune response indicators. Thus, the total number of lymphocytes increased by 3 times, T-lymphocytes – by 1.9 times, and B-lymphocytes – by 2 times. The number of T-helper cells and cytotoxic T-lymphocytes increased by 1.9 times compared to the group of intact animals and 1.6 times compared to selenium-deficient rats. Thus, the course introduction of the molecular complex Se NPs – Chitosan against the background of selenium deficiency was accompanied by inhibition of free radical oxidation processes, activation of the antioxidant system and restoration of the immune status of the organism of laboratory animals. https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1871seleniumpolysacharidesselenium deficiencyimmunity |
| spellingShingle | Marina Verevkina Vadim Goncharov Evgeny Nesmeyanov Olga Kamalova Igor Baklanov Alexander Pokhilko Anzhela Nagapetova Petr Miroshnichenko Application of the Se NPs-Chitosan molecular complex for the correction of selenium deficiency in rats model Potravinarstvo selenium polysacharides selenium deficiency immunity |
| title | Application of the Se NPs-Chitosan molecular complex for the correction of selenium deficiency in rats model |
| title_full | Application of the Se NPs-Chitosan molecular complex for the correction of selenium deficiency in rats model |
| title_fullStr | Application of the Se NPs-Chitosan molecular complex for the correction of selenium deficiency in rats model |
| title_full_unstemmed | Application of the Se NPs-Chitosan molecular complex for the correction of selenium deficiency in rats model |
| title_short | Application of the Se NPs-Chitosan molecular complex for the correction of selenium deficiency in rats model |
| title_sort | application of the se nps chitosan molecular complex for the correction of selenium deficiency in rats model |
| topic | selenium polysacharides selenium deficiency immunity |
| url | https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1871 |
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