Canine Somatic Mutations from Whole-Exome Sequencing of B-Cell Lymphomas in Six Canine Breeds—A Preliminary Study
Canine lymphoma (CL) is one of the most common malignant tumors in dogs. The cause of CL remains unclear. Genetic mutations that have been suggested as possible causes of CL are not fully understood. Whole-exome sequencing (WES) is a time- and cost-effective method for detecting genetic variants tar...
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MDPI AG
2023-09-01
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| author | Sungryong Kim Namphil Kim Hyo-Min Kang Hye-Jin Jang Amos Chungwon Lee Ki-Jeong Na |
| author_facet | Sungryong Kim Namphil Kim Hyo-Min Kang Hye-Jin Jang Amos Chungwon Lee Ki-Jeong Na |
| author_sort | Sungryong Kim |
| collection | DOAJ |
| description | Canine lymphoma (CL) is one of the most common malignant tumors in dogs. The cause of CL remains unclear. Genetic mutations that have been suggested as possible causes of CL are not fully understood. Whole-exome sequencing (WES) is a time- and cost-effective method for detecting genetic variants targeting only the protein-coding regions (exons) that are part of the entire genome region. A total of eight patients with B-cell lymphomas were recruited, and WES analysis was performed on whole blood and lymph node aspirate samples from each patient. A total of 17 somatic variants (<i>GOLIM4</i>, <i>ITM2B</i>, <i>STN1</i>, <i>UNC79</i>, <i>PLEKHG4</i>, <i>BRF1</i>, <i>ENSCAFG00845007156</i>, <i>SEMA6B</i>, <i>DSC1</i>, <i>TNFAIP1</i>, <i>MYLK3</i>, <i>WAPL</i>, <i>ADORA2B</i>, <i>LOXHD1</i>, <i>GP6</i>, <i>AZIN1</i>, and <i>NCSTN</i>) with moderate to high impact were identified by WES analysis. Through a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of 17 genes with somatic mutations, a total of 16 pathways were identified. Overall, the somatic mutations identified in this study suggest novel candidate mutations for CL, and further studies are needed to confirm the role of these mutations. |
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| last_indexed | 2024-03-10T23:07:15Z |
| publishDate | 2023-09-01 |
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| series | Animals |
| spelling | doaj.art-3dbc922dd40c44f78d074688c0727e8f2023-11-19T09:14:25ZengMDPI AGAnimals2076-26152023-09-011318284610.3390/ani13182846Canine Somatic Mutations from Whole-Exome Sequencing of B-Cell Lymphomas in Six Canine Breeds—A Preliminary StudySungryong Kim0Namphil Kim1Hyo-Min Kang2Hye-Jin Jang3Amos Chungwon Lee4Ki-Jeong Na5Laboratory of Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaBiophotonics and Nano Engineering Laboratory, Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Biomedical Laboratory Science, Daegu Health College, Daegu 41453, Republic of KoreaMeteor Biotech, Co., Ltd., Seoul 08826, Republic of KoreaLaboratory of Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaCanine lymphoma (CL) is one of the most common malignant tumors in dogs. The cause of CL remains unclear. Genetic mutations that have been suggested as possible causes of CL are not fully understood. Whole-exome sequencing (WES) is a time- and cost-effective method for detecting genetic variants targeting only the protein-coding regions (exons) that are part of the entire genome region. A total of eight patients with B-cell lymphomas were recruited, and WES analysis was performed on whole blood and lymph node aspirate samples from each patient. A total of 17 somatic variants (<i>GOLIM4</i>, <i>ITM2B</i>, <i>STN1</i>, <i>UNC79</i>, <i>PLEKHG4</i>, <i>BRF1</i>, <i>ENSCAFG00845007156</i>, <i>SEMA6B</i>, <i>DSC1</i>, <i>TNFAIP1</i>, <i>MYLK3</i>, <i>WAPL</i>, <i>ADORA2B</i>, <i>LOXHD1</i>, <i>GP6</i>, <i>AZIN1</i>, and <i>NCSTN</i>) with moderate to high impact were identified by WES analysis. Through a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of 17 genes with somatic mutations, a total of 16 pathways were identified. Overall, the somatic mutations identified in this study suggest novel candidate mutations for CL, and further studies are needed to confirm the role of these mutations.https://www.mdpi.com/2076-2615/13/18/2846canine lymphomawhole-exome sequencingB-cellPARR |
| spellingShingle | Sungryong Kim Namphil Kim Hyo-Min Kang Hye-Jin Jang Amos Chungwon Lee Ki-Jeong Na Canine Somatic Mutations from Whole-Exome Sequencing of B-Cell Lymphomas in Six Canine Breeds—A Preliminary Study Animals canine lymphoma whole-exome sequencing B-cell PARR |
| title | Canine Somatic Mutations from Whole-Exome Sequencing of B-Cell Lymphomas in Six Canine Breeds—A Preliminary Study |
| title_full | Canine Somatic Mutations from Whole-Exome Sequencing of B-Cell Lymphomas in Six Canine Breeds—A Preliminary Study |
| title_fullStr | Canine Somatic Mutations from Whole-Exome Sequencing of B-Cell Lymphomas in Six Canine Breeds—A Preliminary Study |
| title_full_unstemmed | Canine Somatic Mutations from Whole-Exome Sequencing of B-Cell Lymphomas in Six Canine Breeds—A Preliminary Study |
| title_short | Canine Somatic Mutations from Whole-Exome Sequencing of B-Cell Lymphomas in Six Canine Breeds—A Preliminary Study |
| title_sort | canine somatic mutations from whole exome sequencing of b cell lymphomas in six canine breeds a preliminary study |
| topic | canine lymphoma whole-exome sequencing B-cell PARR |
| url | https://www.mdpi.com/2076-2615/13/18/2846 |
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